Biochemical analysis of the pinocytotic process. 3. Subcellular distribution and metabolic effects of ( 3 H)Triton WR-1339.
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Animals↗
Biomedical subjects
R Henning
Publications and source records attributed to R Henning.
Biochemical analysis of the pinocytotic process. I. Isolation and chemical composition of the lysosomal and the plasma membrane of the rat liver cell.
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Animals↗
Biochemical analsis of the inocytotic process. II. Comparison of some enzymes of the lysosomal nd the plasma membrane of the rat liver cel.
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Acid Phosphatase↗
[Intravenous glucose and tolbutamide tolerance test. Methodical and comparative studies].
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Blood Glucose↗
[Intravenous galactose tolerance. Enzymatic substrate determination in capillary blood].
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Adult↗
The relationship between phospholipid biosynthesis, spingosine degradation and serine metabolism.
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Amino Alcohols↗
[Relation of sphingosine degradation and serine metabolism to the biosynthesis of phospholipids].
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Amino Alcohols↗
Studies on the metabolism of (1-3H)erythro-DL-sphingosine (1-3H)4t-sphingenine) during the myelination period of the rat.
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Amino Alcohols↗
Tightly associated lipids may anchor SV40 large T antigen in plasma membrane.
Simian virus 40 (SV40) large T antigen, a multifunctional protein necessary for lytic growth and cell transformation, is located mainly in the nucleus and in small amounts on the cell surface (surface T). Surface T may have a passive role in SV40 tumour rejection by cytotoxic T cells as a component of SV40-TSTA (tumour-specific transplantation antigen). The unusual induction of this immune response by immunizing mice with soluble T antigen led us to investigate the in vitro binding of T antigen to the surface of living cells in more detail. Our results show that native surface T and a minor subset of large T antigen having a high cell surface binding affinity in vitro, behave like integral membrane proteins. Several viral proteins including SV40 T antigen and cellular proteins seem to be linked to fatty acids (acylation). To analyse whether this mechanism is involved in the stable attachment of in vitro-bound T antigen to the plasma membrane of living target cells, we determined the degree of labelling of this molecule by using target cells prelabelled with 3H-fatty acid. Here we report that T antigen extracted from unlabelled SV40-transformed cells (SV80) becomes 3H-labelled after in vitro binding to the cell surface of 3H-palmitate-prelabelled HeLa cells. These results suggest that T antigen attached externally to living cells, may be anchored by tightly linked lipids.
Antigens, Viral, Tumor↗