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Biomedical subjects

R Henn

Publications and source records attributed to R Henn.

17 recordsLinked to original sources
PubMed1992

Some anthropological aspects of the prehistoric Tyrolean ice man.

The corpse of a Late Neolithic individual found in a glacier in Oetztal is unusual because of the intact nature of all body parts that resulted from the characteristics of its mummification process and its protected geographical position with regard to glacier flow. Anthropological data indicate that the man was 25 to 40 years old, was between 156 and 160 centimeters in stature, had a cranial capacity of between 1500 and 1560 cubic centimeters, and likely died of exhaustion.

Animals↗
PubMed1992

Recombinant human C5a induces transcription but not translation of interleukin-1 beta mRNA in human monocytes.

The effect of recombinant human C5a (rhC5a) on the synthesis of interleukin-1 beta (IL 1 beta) was investigated in highly purified human monocytes, isolated by leukapheresis and counter-current elutriation. RhC5a induced IL 1 beta mRNA synthesis in a dose- and time-dependent manner; maximal induction was achieved at 3 h with rhC5a concentrations of 200 to 500 ng/ml. The IL 1 beta mRNA induction with rhC5a was about 75% of the response observed using lipopolysaccharide (LPS) as the stimulus (300 ng/ml). On the other hand, rhC5a in optimal concentrations only weakly stimulated IL 1 beta protein synthesis, as measured by a two-site-directed enzyme-linked immunoassay. When LPS- or C5a-stimulated RNA was used to program IL 1 beta synthesis in an in vitro translation system from rabbit reticulocytes, no difference in translational efficiency was observed. Our results indicate that, in human monocytes, two signals for IL 1 beta gene expression are necessary, one signal for transcriptional activation and a second signal to induce translation of the mRNA.

Complement C5a↗
PubMed1992

[Contact neck gunshot wound without weapon--suicide or homicide?].

To support police in reenacting criminal acts is one of the most important functions of forensic investigation. This case report deals with the discovery of the corpse of a 42-year old man at the bank of the river Inn. First of all the man was supposed to be drowned, but the autopsy showed a gunshot wound of the neck and a shot-through of the brain-stem. Intensive search for the weapon by the police and by divers was unsuccessful and therefore suspicion of homicide arose. The reconstruction of the act at the site of discovery of the corpse led to the discovery of the weapon and the case became clear to be a suicide.

Adult↗
PubMed1992

[Epidemiology of fatalities in Alpine skiing--(1987-1990)].

At the Institute of Forensic Medicine, University Innsbruck, 78 cases of fatal accidents and of sudden death were examined which occurred during alpine skiing between 1987 und 1990. An increase of fatal accidents during skiing was observed in the past few years. This fact is related to the rising utilization of summer skiing areas. In about three quarters of the cases death as consequence of an accident was observed. One quarter died a sudden death. In this group myocardial infarction is dominant, which is an indication of an enhanced load of the cardiovascular system during skiing in higher altitudes. In the group with fatal accidents cranio-cerebral traumas including medulla injuries are dominant.

Accidents↗
PubMed1992

[Forensic medicine aspects of death caused by hypothermia in high altitude].

Macro- and micromorphological findings in cases of death from hypothermia are very questionable and unreliable diagnostical signs. We investigated 42 cases of death from hypothermia that occurred in mountain-ranges between 1974 and 1990. All cases showed erosions of the gastric mucosa ("Wischnewsky-spots"), in one case only we found bleedings in the ileopsoas muscles. In most cases hypothermia was based on natural disease or intoxication. Nine alcoholized persons showed a mean alcohol concentration in the blood of 1.07% and in accordance to a relatively long agonal period the alcohol concentration in urine was considerably higher.

Accidents↗
PubMed1992

[Discovery of human bones at the Prägratkees (Tyrol) 1953 and 1990].

At the Prägratkees (Granatspitz, Tyrol) human bones, pieces of equipment and garment were found at the same site in 1953 and 1990. According to the forensic investigations, the bones were attached to a 37 year-old mountain climber, who has been missed since 1936. Because of the fact that between both discoveries an interval of 37 years elapsed, it is doubtful whether the bones are from one person. The forensic conclusion, that the bones are attached to one person could be supported by the glaciological situation of this glacier.

Adult↗
PubMed1992

Recombinant human C5a induces transcription but not translation of interleukin 1 beta mRNA in human monocytes.

The effect of recombinant human C5a (rhC5a) on the synthesis of interleukin 1 beta (IL-1 beta) was investigated in human monocytes, isolated by leukapheresis and countercurrent elutriation. rhC5a induced IL-1 beta mRNA synthesis in a dose- and time-dependent manner. Maximal induction was achieved at 3 h with rhC5a concentrations of 200 to 500 ng/ml. The maximal rhC5a-stimulated mRNA induction was about 75% of that observed using LPS as the stimulus (300 ng/ml). The inducing activity of rhC5a could be neutralized by preincubation with a polyclonal anti-C5a antibody. On the other hand rhC5a in optimal concentrations only weakly stimulated IL-1 beta protein synthesis, as measured by a two-site directed enzyme-linked immunoassay. When compared on Northern blots, a slightly reduced mobility of C5a-stimulated IL-1 beta mRNA was observed relative to LPS-stimulated RNA. To exclude the possibility that structural defects in the IL-1 beta mRNA are responsible for the weak translational efficiency after C5a stimulation a primer extension experiment was performed. No difference in the length of the extended fragment was detected between LPS and C5a-stimulated RNA, suggesting that the 5'-regions of the RNAs are identical. When LPS- or C5a-stimulated RNA was used to program IL-1 beta synthesis in an in vitro translation system from rabbit reticulocytes, no difference in translational efficiency was observed. Our results indicate that in human monocytes two signals for IL-1 beta gene expression are necessary, a signal for transcriptional activation and another signal to induce translation of the mRNA.

Blotting, Northern↗
PubMed1991

[Typical skiing injuries].

In a retrospective study of alpine skiing injuries which were seen at the Trauma Center of the University of Innsbruck in the years 1960-1991 a large change in "typical skiing injuries" was found. In the group of severe injuries, a large shift from the lower to the upper extremity (50.37%) has taken place. 3.93% of all patients were categorized as polytrauma patients in PTS groups II, IV, and 1.19% of all skiers died immediately after the injury. While 191 fractures and 118 capsular ligament injuries of the upper extremity were seen, 135 fractures and 229 severe capsular ligament lesions (mainly knee) of the lower extremity had to be treated.

Adolescent↗
PubMed1991

Postmortem neopterin concentrations: comparison of diagnoses with and without cellular immunological background.

Increased neopterin levels in urine and serum of living humans indicate an activation of the cellular immune system. We investigated 119 urine and 48 serum samples from 129 corpses taken at necropsy; 29 cases with a background of cellular immune activation were compared to 100 corpses with no such indication. Our investigations show the feasibility of postmortem neopterin measurements. However, different kinetics of serum and urine concentrations after death were observed. In addition, the data show that urine and serum neopterin concentrations were significantly higher when cellular immunological abnormalities were present when compared to the control group and to living healthy controls. The findings suggest, that increased postmortem urine neopterin concentrations in necropsy indicate pathological processes linked with cellular immune activation.

Adolescent↗
PubMed1991

Unusual finding in a water-logged corpse--hyperchylomicronemia or pulmonary fat embolism?

During excavation work at the bank of the River Inn the corpse of a 32-year-old alcoholic male was recovered. Head injuries suggested a crime of violence. Postmortem examination demonstrated conspicuous milky turbidity of the blood, which was found by laboratory testing to be due to hyperchylomicronemia. The findings are interpreted and their relevance to the determination of postmortal head injuries is discussed. The inadequacy of double-edged knife specimens and frozen-section biopsies for the estimation of pulmonary fat embolism as a vital reaction is considered.

Adult↗
PubMed1991

Postmortem evaluation of serum and urine neopterin concentrations.

Cellular immune response is accompanied by the release of neopterin. Increased neopterin levels in urine and serum are observed in patients during viral infections, autoimmune diseases, and allograft rejections and certain malignant diseases. We investigated postmortem neopterin concentrations in urine and serum samples taken from 32 corpses 3 to 69 h (mean 19.3 h) after death. Urine neopterin concentrations in corpses are similar to those of healthy live controls and are independent of the time after death. In contrast, serum neopterin concentrations are frequently greatly increased in corpses, and the levels are higher in sera collected more than 10 h after death in comparison with samples obtained earlier. Neopterin measurement in urine and serum samples of corpses is feasible. It appears likely that urine neopterin concentrations could aid the diagnosis of inflammatory diseases in corpses.

Adult↗
PubMed1991

Fatal accidents on glaciers: forensic, criminological, and glaciological conclusions.

The rare event of a corpse immersed in glacier ice becoming exposed on a glacier surface is closely connected with the glaciological conditions at the scene and the site of the accident. Provided that the time since death is known, certain questions relative to the circumstances of a mountain accident can only be answered by considering glaciological aspects. How the scene of an accident can be reconstructed by inference from the site of discovery is discussed by means of three exemplary cases that occurred on Tyrolean glaciers (Austria) during the past 40 years: (1) Two corpses were discovered close above the equilibrium line in the accumulation area after 25 years. The two victims had fallen down a rock face after the breaking off of a cornice and had come to rest in the uppermost part of the accumulation area. (2) A victim was discovered in the lower ablation area 8 years after falling down a crevasse in the middle part of the ablation area. (3) A female alpinist was discovered at the very end of the glacier after 29 years; it was concluded that the accident must have happened in the accumulation area.

Abdominal Injuries↗
PubMed1989

An assay for the detection of interleukin-1 synthesis inhibitors: effects of antirheumatic drugs.

The monokines interleukin-1 beta and alpha (IL-1) play a central role in the connective tissue destruction of many chronic inflammatory diseases. A high capacity screening assay for the detection of inhibitors of IL-1 biosynthesis has been established. Normal human monocytes were obtained by leukapheresis and elutriation. IL-1 beta and alpha biosynthesis was stimulated with LPS, and cell-associated and secreted IL-1 beta and IL-1 alpha were measured by specific immunoassays (ELISA). The mean total IL-1 beta (cell-associated and secreted) production in 18 different donors was 11 ng/10(6) cells (range 1.2-28.8). Secreted IL-1 beta represented 31 to 86% of the total IL-1 beta. More IL-1 alpha than IL-1 beta was produced but, unlike IL-1 beta, IL-1 alpha was poorly secreted. The steroids prednisolone and dexamethasone, gold (sodium aurothiomalate) and chloroquine were potent inhibitors of the IL-1 production. Mean IC50 values of 180 nM (range 2.5 nM-1 microns), 10 microM (range 6-20 microM) and of 75 microM were found for prednisolone, gold and chloroquine, respectively. Above 5 microM, the non-steroidal anti-inflammatory compounds indomethacin and BW755C increased IL-1 beta biosynthesis. Nordihydroguaiaretic acid inhibited the level of the secreted form of IL-1 beta, but tended to increase the cell-associated level. D-Penicillamine (up to 6 mM), cyclosporin A (up to 1 microM) and methotrexate (up to 12 microM) inhibited neither cell-associated nor secreted IL-1 beta levels. This high capacity assay, which is insensitive to classical NSAIDs, may serve in the detection and characterization of new classes of anti-inflammatory compounds.

Anti-Inflammatory Agents↗
PubMedDate not supplied

[Diagnostic criteria in classical infantile subacute necrotizing encephalomyelopathy (Leigh's disease)].

Subacute necrotizing encephalomyelopathy (SNE; Leigh's disease), though a defined entity in neuropathological and morphological terms, is characterized by high clinical heterogenity. SNE of infancy can be defined and diagnosed on the basis of clinical symptoms more readily than juvenile and adult forms. Four patients with SNE displayed combinations of recurrent vomiting, difficulty in swallowing, failure to thrive, impairment of ocular innervation, muscle tone regulation and central regulation of respiration. These symptoms, particularly in combination, point to a disorder of the brainstem and basal ganglia. In addition, all four patients were suffering from lactic acidosis. Other possible indicators, but not in all 4 patients, were abnormal CT brain scans, impaired nerve conduction velocity, elevated CSF protein levels and enlarged mitochondria in muscle cells. Abnormal brainstem auditory evoked potentials have proved to be one of the best criteria for early diagnosis of brainstem lesions. Enzyme assays of pyruvate degradation in cultured skin fibroblasts revealed diminished activity of the pyruvate dehydrogenase complex in one patient (52 pmol/mg protein x min; median range 313, 82-917, n: 58). This paper summarizes the findings and proposes primary and secondary criteria of assistance in establishing an initial clinical diagnosis of infantile SNE. As no common pathogenetic mechanisms have yet been recognized and no uniform diagnostic markers are yet available, the diagnosis still requires confirmation by histological examination of brain and brainstem, as was the case with all four patients presented.

Brain↗