Biomedical subjects

R Hay

Publications and source records attributed to R Hay.

34 records · Page 2Linked to original sources

PubMed1986

Cross-linking analysis of yeast mitochondrial outer membrane.

By enrichment of contact sites between the two mitochondrial boundary membranes it has been shown that this fraction contained a high activity of glutathione transferase and hexokinase which was bound to the outer membrane pore protein (Ohlendieck, K. et al. (1986) Biochim. Biophys. Acta 860, 672-689). Therefore, an interaction between the three proteins in the contact sites has been suggested. Cross-linking experiments with isolated outer membrane of yeast mitochondria show that glutathione transferase and the pore protein are already associated in the free outer membrane. Porin appeared to adopt four different oligomeric complexes in the membrane, including interactions with a 14 kDa polypeptide, which has glutathione transferase activity. The latter polypeptide could be phosphorylated by intrinsic or extrinsic protein kinases, while the porin itself was not phosphorylated. Yeast hexokinase, when bound to the outer membrane, was able to cross-link to the pore protein.

Cell Membrane↗

PubMed1986

Neurofibromatosis.

Since first so named in 1882, von Recklinghausen's disease has been reported to have a wide variety of associated defects, including the more common intracranial and extracranial neuromas and the less common intracerebral tumors and large artery ectasias, aneurysms, and spontaneous fistulas, in addition to a variety of small vessel tumors and arteriovenous fistulas. A case is reported with the association of a vertebral artery spontaneous fistula along with spontaneous spinal fluid rhinorrhea and a Chiari type I malformation. Review of the literature reveals no report of either of the latter two associated with von Recklinghausen's disease.

Arteriovenous Fistula↗

PubMed1986

Alterations in molecular structure of renal mitochondria associated with cyclosporine A treatment.

We examined whether ultrastructural changes in renal mitochondria associated with Cyclosporine A treatment might reflect underlying alterations in mitochondrial molecular structure. A nonrejected renal transplant removed from a patient treated with Cyclosporine A showed a decreased level of beta subunit antigen of mitochondrial F1-ATPase compared to controls. This decrease was more marked in the medulla than in the cortex (56% vs. 70% of pooled controls). Spontaneously hypertensive rats treated with a high dose of Cyclosporine A showed a similar decrease of beta-subunit antigen in the renal medulla and decreased levels of two subunit antigens of cytochrome c oxidase, but had increased medullary levels of the alpha subunit antigen of the F1-ATPase. Such changes were not detected in a normotensive strain of rats. Our data indicate that Cyclosporine A administration is associated with structural alterations in renal mitochondria at the molecular level, predominantly in the medulla, and that additional renal damage due to ischemia or hypertension may predispose to this cyclosporine effect.

Adenosine Triphosphatases↗

PubMed1986