Learning, learning, forever learning.
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Adaptation, Psychological↗
Biomedical subjects
R Haugen
Publications and source records attributed to R Haugen.
The effect of feeding and fasting on the activity of acyl-CoA: cholesterol acyltransferase in rat small intestine.
The purpose of the present study was to test if the microsomal acyl-CoA:cholesterol acyltransferase (ACAT) in rat small intestine is regulated under physiological conditions. Two previously described in vitro assays were used, both based on the esterification of endogenous cholesterol with exogenous acyl-CoA, performed or generated during the incubation. The important and consistent finding with rats on normal diet was an increase in ACAT activity with fasting and a decrease with feeding. Independent of the assay used, the ratio between ACAT activity in night-fasted and night-fed animals was about 2 (P less than 0.005). When the fasting period was extended to 36 h a corresponding difference was found whether the ACAT assay was based on preformed [1-14C]oleoyl- or [1-14C]palmitoyl-CoA as acyl-donor (P less than 0.05). The microsomal content of unesterified cholesterol was higher in fasted than fed animals, suggesting that availability of this substrate might be a factor in the regulation of rat intestinal ACAT activity.
Acyl Coenzyme A↗
Coenzyme-A-dependent esterification of cholesterol in rat intestinal mucosa.
Rat intestinal mucosa contains an enzyme which catalyses the esterification of cholesterol. The enzyme is CoA-dependent and probably an acyl-CoA: cholesterol acyltransferase (ACAT) (E.C.2.3.1.26). Maximal activity in vitro is obtained when long chain acylcarnitines, CoA and carnitine palmityltransferase are used as an acyl-CoA generating system. The enzyme is localized in the microsomal fraction, has a pH optimum between 6.4-7.2, and oleate is the preferred acyl group. The activity of the enzyme is highest in the proximal jejunum, and has a capacity that can account for all cholesteryl esters found in intestinal rat lymph.
Acetyl Coenzyme A↗
Water and electrolyte balance following gastric resection.
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Acid-Base Equilibrium↗