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Biomedical subjects

R Hartmann

Publications and source records attributed to R Hartmann.

165 records · Page 10Linked to original sources

[Morphologic studies of spermatozoa in disorders of motility].

On the basis of microscopical examination and further differentiation by special stain techniques, a new classification of defects of midpieces and tails of spermatozoa will be presented: First degree disorders i.e. abnormalities of the staining behaviour and minor structural defects, second degree disorders revealing breakes and coiling of spermatozoa tail, and finally severe third degree disorders only exhibiting a rudimentary anlage of the terminal segment of spermatozoa. The staining abnormalities of the first degree disorders are characterized by nonreactivity to the Polychrome stain of the Papanicolaou technique as well as to scarlet red stain of the Shorr technique. Although the majority of these spermatozoa do not exhibit morphological aberrations, they have to be regarded completely immotile. Considering this classification, we have observed a highly significant correlation between morphological findings of midpieces and tails and the total motility as well as the progressive motility of spermatozoa.

Autoimmune Diseases↗

Human sperm motility--enhancement and inhibition measured by laser Doppler spectroscopy.

Laser Doppler spectroscopy is an excellent instrument to measure motility parameters of human spermatozoa. Experiments have been performed by the aid of a now commercially available instrumental setup called LAZYMOT to study enhancement and inhibition as well as influences of different physical treatments on the motility of human spermatozoa. The motility enhancing influence of Kallikrein (up to 40% with 4 KU/ml) and prostaglandin E2 could be proven, thereby verifying some sort of mechanism of action within the Kallikrein-Kinin system. Furthermore tested substances in this context were bradykinin (no effect), caffeine (enhancement), salicylic acid (slight decrease), and other prostaglandins (no effect). The influence of washing, centrifugation, resuspension liquid, and temperature has been examined. It turned out that the lowest motility degradation is to be achieved by washing twice at a pH value of 7.4 at forces of 200 g for 10 min each time. The optimal temperature during this process and the measurements thereafter was found to be 36 degrees C. Unsaturated fatty acids like arachidonic acid have been found to be very strong motility inhibiting substances, which can totally block any movement of spermatozoa.

Bucladesine↗