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R Haque

Publications and source records attributed to R Haque.

78 records · Page 5Linked to original sources
PubMed1967

Purification and properties of staphylococcal delta-hemolysin. I. Production of delta-hemolysin.

Concentrated preparations of staphylococcal delta-hemolysin were obtained by growing selected hemolytic colonies from the 146P strain of Staphylococcus aureus on dialysis membranes laid over Brain Liver Heart agar plates at 37 C for 20 hr under 10% CO(2) and harvesting the growth from five such membranes in 1.0 ml of deionized distilled water. Incubation in a humid environment facilitated this harvesting procedure. Incubation longer than 40 hr or incubation under CO(2) higher than 10 to 20% gave lower yields of delta-lysin. Addition of a sugar, fermented by the organisms, resulted in lower yields of delta-hemolysin. Agar, although separated from the growing cells by the dialysis membrane, did potentiate delta-hemolysin production. Addition of 0.1% agar to the inoculum further enhanced this potentiation. delta-Hemolysin produced in broth or semisolid cultures was excessively diluted with the media. Dialysis membranes prevented this dilution and thus yielded concentrated preparations of delta-hemolysin.

Agar↗
PubMedDate not supplied

Percutaneous balloon pericardiotomy: non-surgical treatment for patients with cardiac tamponade.

We present two patients with recurrent large pericardial effusions who were treated with balloon pericardiotomy. This is a novel treatment in which a non-surgical pericardial window is created via the percutaneous subxiphoid route using a balloon dilating catheter. Our two patients initially had standard pericardiocentesis performed. However, large pericardial effusions soon returned in both patients so further and more definitive treatment was required. Following balloon pericardiotomy, neither patient developed a significant pericardial effusion after months of follow up. Given our experience with percutaneous balloon pericardiotomy, as well as the experience of others, we believe that this approach will become the preferred treatment for large pericardial effusions or tamponade, especially in patients with cancer.

Aged↗