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Biomedical subjects

R Hampl

Publications and source records attributed to R Hampl.

At least 91 records · Page 5Linked to original sources
PubMed1991

The determination of 11 beta-hydroxyandrostenedione in human follicular fluid and plasma.

The development of a chromatographic/immunoassay method is presented for the measurement of 11 beta-hydroxyandrostenedione (11 beta-OH-A4) in ovarian follicular fluid (FFL) and plasma from women undergoing embryo transfer for in vitro fertilization. This method incorporates high-performance liquid chromatography (HPLC) and permits the simultaneous measurement of other steroids from a single sample in order to assess the intraovarian environment. Authenticity of 11 beta-OH-A4 in follicular fluid was confirmed using selected ion monitoring (SIM) gas chromatography/mass spectrometry (GC/MS). Our results demonstrate a mean concentration of 18.6 nmol/l in follicular fluid compared with 3.2 nmol/l in plasma. The origin of 11 beta-OH-A4 in follicular fluid requires further investigation but these findings supports the hypothesis of ovarian 11 beta-hydroxylase activity on C19 steroids.

Adrenal Glands↗
PubMed1991

Recidivous offence in sadistic homosexual pedophile with karyotype 48, XXXY after testicular pulpectomy. A case report.

The case of recidivous sexual offender with genetically caused mental retardation and primary hypogonadism (Klinefelter's syndrome with karyotype 48, XXXY) is described. He was examined after sadistic abuse of a boy aged 13 that he had committed 19 years after performed testicular pulpectomy. Plasmatic level of testosterone was found 4x higher than mean level in men after orchidectomy. Histological examination of residual scrotal tissues proved that the source of androgens were hyperplastic nodules of extratesticular Leydig cells.

Biopsy↗
PubMed1991

Observations on the biological activity of epitestosterone.

Epitestosterone, a 17 alpha-epimer of testosterone is a normal constituent of body fluids in many species including man. It has long been believed that it is devoid of any biological significance. However, it is now demonstrated that in in vivo experiments on castrated male mice it counteracts the action of testosterone on androgen-dependent organs. In vitro experiments show that on the overall antiandrogenicity of epitestosterone participate true antiandrogenic action due to the binding to androgen receptors, strong 5 alpha-reductase inhibiting activity as well as a weak antigonadotropic activity. Epitestosterone is devoid of any embryotoxicity as checked by chick embryo-toxicity screening test.

5-alpha Reductase Inhibitors↗
PubMed1991

Influence of testosterone isobutyrate on serum lipoproteins during replacement therapy of hypogonadal men.

Replacement therapy of hypogonadal men with testosterone isobutyrate, 100 mg by the i.m. route every two weeks, does not lead to a permanent significant change of the lipoprotein spectrum and to an increased risk of stereogenesis. The expected changes in the liver lipase activity and in the lipoprotein spectrum under the influence of the administered androgens are obviously suppressed by the antagonistic action of estrogens formed by conversion from androgens.

Adult↗
PubMed1990

Daily profiles of salivary cortisol in hydrocortisone treated children with congenital adrenal hyperplasia.

Daily profiles of salivary cortisol were determined in 14 cortisol-treated children with congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency, and in 5 healthy sibs. The results showed considerable individual variation irrespective of the dose of hormone, reflecting the different rates of cortisol metabolism and transport. Maximum salivary cortisol levels were reached 1-2 h following oral administration of hydrocortisone. The determination of salivary cortisol may be useful for optimal dosage timing, i.e. to imitate the daily rhythm of normal cortisol secretion.

17-alpha-Hydroxyprogesterone↗
PubMed1990

11 beta-hydroxyandrostenedione in human amniotic fluid.

11 beta-Hydroxyandrostene-3,17-dione (11 beta-hydroxyandrostenedione), a potential marker for prenatal diagnosis of CAH, has been for the first time determined in amniotic fluid obtained from 68 women who later delivered children without endocrine disorders. Its concentration averaged 0.96 +/- 0.31 (S.D.) (range 0.48-1.85) nmol/l and did not depend on the fetal sex. There was mild, insignificant increase of these values along with duration of pregnancy.

Adrenal Hyperplasia, Congenital↗
PubMed1990

Hormonal status and sexual behaviour of 16 men after surgical castration.

Sixteen castrated sexual offenders were investigated blood plasma levels of testosterone [T], dihydrotestosterone [DHT], luteinizing hormone [LH] and follicle-stimulating hormone [FSH]. Levels of T and DHT were significantly decreased in comparison with normal values. Levels of both gonadotropic hormones were significantly elevated. There was a great reduction in the occurrence of orgasm and ejaculation in castrated men. Only six of these sixteen men were able to reach orgasm. Only two of these men stated that they had a small amount of ejaculate by orgasm. Phalloplethysmographic [PPG] examination were provided with electrocapacitance PPG equipment. In only two men investigated was there no specific erotic reactions to visual stimuli. PPG responses in 8 men were small, in 4 average and in 2 only were these reactions high. No changes in sexual orientation were found after castration. In most men after surgical castration sexual arousal is preserved. Low, with low frequency of orgasm and very low sexual interest.

Adult↗
PubMed1990

[Diagnosis of the heterozygote carrier state in 21-hydroxylase deficiency using steroids].

In an attempt to elaborate a method for screening heterozygous carriers of inborn adrenal hyperplasia caused by insufficiency of steroid 21-hydroxylase, the authors examined in 24 obligatory heterozygotes and in seven controls the concentrations of four steroids. Cortisol (F), 11 beta-hydroxyandrostendione (11-OH), 17 alpha-hydroxyprogesterone (17-OH) and androstendione (A) were estimated by the RIA method using non-commercial antisera. In the examined subjects an abbreviated ACTH test was made and the mentioned parameters were assessed at times 0, 30 and 60 minutes. The results (nmol/l) were expressed by the relationship R = F.11-OH/17-OH.A for all investigated time intervals. The coefficient R60 or difference of coefficients delta R = R60-R0 differed significantly and overlapped to a minimum extent in the group of heterozygotes and controls. The authors assume that the suggested method for the detection of carriership of 21-hydroxylase insufficiency could be used in genetic counselling.

17-alpha-Hydroxyprogesterone↗
PubMed1990

[Use of steroid indicators in monitoring the treatment of congenital adrenal hyperplasia].

The authors divided forty children and adolescents with inborn adrenal hyperplasia caused by a block of 21-hydroxylase of steroids (CAH) according to clinical criteria into adequately and inadequately treated. Substitution treatment with hydrocortisone or hydrocortisone combined with fludrocortisone was administered three times a day in individual doses. In order to find an adequate indicator of the adequacy of therapy, the authors assessed in addition to total urinary 17-oxosteroids also 17 alpha-hydroxyprogesterone, androstenedione and 11 beta-hydroxyandrostenedione in serum. The values of urinary 17-oxosteroids did not correspond to the clinical condition of the children; in younger children three was a high percentage of falsely positive and in older children of falsely negative results. As to serum steroids, the most suitable indicator for monitoring was 17-hydroxyprogesterone. The authors consider its levels above 30 nmol/l as a basis for consideration of raising therapeutic doses, while levels below 2.2 nmol/l signalize possible overdosage of the drug. Concurrently elevated levels of 17-hydroxyprogesterone and androstenedione are found in particularly inadequately treated children. 11 beta-hydroxyandrostenedione is not a useful indicator for monitoring of CAH treatment. In any case it is necessary, when controlling CAH therapy, to select an individual procedure and to evaluate results in the context with the patient's clinical condition.

17-Ketosteroids↗
PubMed1989

[Does epitestosterone function as an endogenous antiandrogen?].

The action of the endogenous steroid epitestosterone administered to castrated male mice substituted with testosterone propionate is manifested by reduced weight increments and a reduced relative weight of their seminal vesicles and kidneys. Epitestosterone in vitro displaces androgens from their bond with receptors in cytosol from rat prostates and markedly inhibits the testosterone transformation to the more effective androgen dihydrotestosterone. Epitestosterone can be thus defined as a true endogenous antiandrogen; to its action at the receptor level a potent inhibitory effect on 5 alpha-reductase must be added.

Androgen Antagonists↗
PubMed1989

Epitestosterone--an endogenous antiandrogen?

Epitestosterone (17 alpha-hydroxy-4-androsten-3-one), a normal constituent of human plasma and urine, prevents the testosterone induced changes in body weight and in organ weights of seminal vesicles and kidney of castrated male mice. It competes with methyltrienolone in the binding to rat prostate cytosol (Ki = 29.8 nmol.l-1. It inhibits also the activity of 5 alpha-reductase from rat prostate pellet (Ki = 1.2 mumols.l-1). Epitestosterone can be considered as a weak antiandrogen in the term of displacement of androgen from receptor binding and as an efficient inhibitor of 5 alpha-reductase.

Animals↗
PubMed1989

[Comparison of the excretion of 3 androgens registered in Czechoslovakia].

Hypogonadal men of different aetiology were administered testesterone undecanoate (Andriol, Organon) and methyltestosterone (Agovirin coated tablets, Spofa) for three months alternatively. By means of specific radioimmunoassays excretion of testosterone and methyltestosterone respectively in the urine was observed in 20 and 5 patients respectively. The course of excretion of both the preparations is possible to illustrate by decreasing curves with apparent half-lives 1.74 and 3.90 day respectively. Analogous observation of testosterone excretion after three-month administration of testosterone isobutyrate (Agovirin depot) to five female-to-male transsexual patients was observed. Excretion course shows the maximum on 27th day after the treatment was discontinued and it is not possible to illustrate it by a monotonous curve.

Adolescent↗
PubMed1989

Estrogen receptors in male gynecomastia.

Nineteen tissue specimens obtained at surgery of male mammary gland due to persisting gynecomastia were assessed for the presence of estrogen receptors. In 13 cases the receptors were detected with the use of charcoal-dextran technique and 3H-estradiol as a ligand, the detection limit of such method being 0.2 fmol/mg of total protein. The average number of estrogen binding sites (mean +/- S. D.) was 2.49 +/- 2.47 fmol/mg total protein with the apparent association constant 2.8 X 10(9) 1 mol-1. The specificity of ligand binding to the receptor was confirmed by gradient centrifugation.

Adolescent↗
PubMed1988

Hormonal changes in tamoxifen treated men with idiopathic oligozoospermia.

Three months of tamoxifen treatment of 43 men with idiopathic oligozoospermia, out of which 20 completed the study, resulted in a significant enhancement of sperm motility, but the improvement of sperm parameters was in no relation to the FSH response to short time tamoxifen treatment. There was a significant increase of testosterone, estradiol, LH, FSH, SHBG, 17 alpha-hydroxy-progesterone and also of 11 beta-hydroxyandrostenedione, an androgen of exclusively adrenal origin, during the treatment and (with the exception of the latter), on the first week after discontinuation of the therapy. Significantly elevated testosterone and SHBG concentrations were retained still 9 weeks after finishing of the therapy. The results confirm that tamoxifen treatment provides conditions more favourable for conception and demonstrate that also adrenal steroidogenesis is positively influenced by this antiestrogen.

17-alpha-Hydroxyprogesterone↗
PubMed1988

Steroid and steroid regulating hormones in human male castrates.

Testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone and its sulphate, 11 beta-hydroxyandrostenedione, 17 alpha-hydroxyprogesterone, cortisol, LH, FSH and sex hormone binding globulin (SHBG) were determined in 16 otherwise healthy male castrates, orchidectomized for the forensic reasons. Their hormone levels were compared with those, obtained in normal males of the same age category. There were found in the castrates decreased levels of testosterone, dihydrotestosterone, dehydroepiandrosterone sulphate and androstenedione, unaltered concentrations of cortisol and dehydroepiandrosterone, whereas the levels of 11 beta-hydroxyandrostenedione, 17 alpha-hydroxyprogesterone, both gonadotrophins and SHBG were increased. The results point to the importance of the selective increase of the incretory activity of the adrenal cortex in situations, when the gonadal function is impaired or missing.

Adrenal Cortex↗