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Biomedical subjects

R Haeckel

Publications and source records attributed to R Haeckel.

At least 109 records · Page 6Linked to original sources
PubMed1976

Persistence of high intestinal lactase activity (lactose tolerance) in Afghanistan.

Two hundred and seventy apparently healthy adult subjects from Afghanistan, mainly from the central and eastern parts of the country, were subjected to a lactose tolerance test. The change of blood glucose from the fasting concentration at 20 min after the administration of lactose showed a bimodal distribution. Forty-seven subjects had a rise of blood glucose concentration of more than 1.1 mmol/l and were classified as persistence of high intestinal lactase activity (PHILA), a term which lays emphasis on the fact that high lactase activity in the adult is an unusual state whose prevalence in some populations requires explanation. In the Afghan sample there were no significant differences of the frequency of PHILA in different ethnic groups.

Adult↗
PubMed1976

The use of aldehyde dehydrogenase to determine H2O2 -producing reactions. I. The determination of the uric acid concentration.

The sequence of reactions catalyzed by uricase, catalase and aldehyde dehydrogenase for determination of the uric acid concentration was evaluated for human serum and urine samples. More than 60 substances were tested for possible interferences. Alcohol dehydrogenase, formaldehyde and homogentistic acid can disturb the proposed assay, but at concentrations which are not usually encountered in human serum or urine. In the presence of protein at least 99% of the uric acid was recovered.

Alcohol Oxidoreductases↗
PubMed1976

The reliability of a mechanized procedure (Perkin-Elmer C4) for the Enzymatic determination of uric acid according to Kageyama.

The enzymatic determination of the uric acie concentration in urine and serum according to Kageyama ((1971), Clin. Chim. Acta 31, 421--426), which excludes the deproteinization of samples, was adapted to the C4 automatic analyzer (Perkin-Elmer). The reliability of this procedure and its correlation with an UV-method were investigated. The interaction from-sample-to-sample was considerable, but this could be reduced by the addition of Brij-35. The recovery of uric acid added to protein-containing samples (about 96%) was better than with the UV-method. Novaminsulfone was the only substance tested which interfered significantly.

Autoanalysis↗
PubMed1976

Evaluation of the Abbott Bichromatic Analyzer 100 (A proposal for an evaluation scheme).

This report summarizes a one year evaluation of Abbott's ABA 100, with respect to mechanical parts (syringe plates, precision and linearity of photometry, band width of several filters, multicuvet precision, temperature control) and the reliability of several methods (endpoint procedures: determination of the glucose concentration with hexokinase- and the glucose dehydrogenase method, and of the protein concentration; enzyme activities: alanine and aspartate aminotransferase, creatine kinase, alkaline phosphatase). The critical batch size was estimated as an indicator of economy (about 40 samples per day for the glucose concentration). Various aspects of the instrument are discussed with respect to its use in clinical chemistry.

Autoanalysis↗
PubMed1976

A new enzymatic determination of cholesterol. The use of aldehyde dehydrogenase to measure H2O2 producing reactions, II.

Cholesterol oxidase, coupled with the catalase and aldehyde dehydrogenase, is proposed for the determination of cholesterol. The main advantages of this procedure over comparable methods employing cholesterol oxidase are its short reaction time and the use of NADP which permits the direct calculation of the cholesterol concentration from the absorbance value.

Aldehyde Oxidoreductases↗
PubMed1976

Inhibition of gluconeogenesis by sodium nitroprusside in the perfused guinea pig liver.

Sodium nitroprusside was found to be a very strong inhibitor of the glucose formation from lactate in the perfused guinea pig liver. Hepatic cell respiration and oxidative phosphorylation appeared to be simultaneously suppressed by this compound. The pattern of some hepatic metabolite concentrations was similarly affected by nitroprusside as described for biguanides and pyridinium chloride derivatives. Cyanide had to be added to the perfusion medium in a tenfold higher concentration to achieve a comparable inhibition of gluconeogenesis from lactate.

Animals↗