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R H Glew

Publications and source records attributed to R H Glew.

At least 181 records · Page 10Linked to original sources
PubMed1981

Isolation, characterization and localization of a 45 000 molecular weight, soluble glycoprotein from the lung in pulmonary alveolar proteinosis.

A carbohydrate-rich, water-soluble glycoprotein has been isolated in pure form from delipidated lung lavage fluid from a patient with pulmonary alveolar proteinosis, in a three-step procedure involving ion-exchange and gel filtration chromatography. The molecular weight of the glycoprotein was determined to be 45 900 by sedimentation equilibrium analysis in the analytical ultracentrifuge and 45 000 by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, indicating a single polypeptide chain. Nearly half of the mass of the glycoprotein is comprised of carbohydrate that is contributed by 24 residues sialic acid, 23 residues N-acetylglucosamine, 6 residues N-acetylgalactosamine, 19 residues galactose, 4 residues mannose, 1 residue fucose and 1 residue glucose per mol. Unlike a number of collagen-related glycoproteins that have been isolated by others from insoluble lung contents in pulmonary proteinosis, the water-soluble glycoprotein described in the present report does not contain hydroxyproline or hydroxylysine and contains less than 10% of its amino acid residues as glycine. Using rabbit antibodies directed against our purest preparation of material and an immunoperoxidase staining procedure, the 45 000 molecular weight glycoprotein was localized to the thin film of fluid lining the surfaces of alveoli in normal human lungs.

Amino Acids↗
PubMed1981

Isolation and characterization of rat alpha-1-antitrypsin.

From 330 ml of rat serum, 222 mg of alpha-1-antitrypsin have been isolated and purified with an overall yield of approximately 20%. The preparation was homogeneous by the criteria of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, sedimentation equilibrium centrifugation, and immunoelectrophoresis. Rat alpha-1-antitrypsin exhibited Mr = 47,000 +/- 1,500 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 45,000 +/- 1,000 by equilibrium ultracentrifugation; the sedimentation coefficient (s20,w) was 3.29. Rat alpha-1-antitrypsin exhibited a trypsin-combining ratio (moles of trypsin inhibited/mol of alpha-1-antitrypsin) of 0.88. Rat alpha-1-antitrypsin showed significant differences in amino acid composition when compared to human alpha-1-antitrypsin, particularly in lysine, glutamic acid, arginine, methionine, and tyrosine content. Rat alpha-1-antitrypsin contains 14.3 residues/mol of N-acetylglucosamine, 5.0 residues/mol of mannose, 4.2 residues/mol of galactose, and 5.8 residues/mol of sialic acid. Monospecific antibody produced in a rabbit against our purest preparation of rat alpha-1-antitrypsin does not cross-react immunologically against human, calf, fetal calf, mouse, or chicken sera. The availability of a pure preparation of rat alpha-1-antitrypsin as well as the specific alpha-1-antitrypsin antibody will facilitate studies on the biosynthesis and secretion of this important protease inhibitor in an appropriate animal model.

Amino Acids↗
PubMed1981

CSF lysosomal hydrolase activity as an aid in the diagnosis of bacterial meningitis.

The activity of the lysosomal enzymes acid phosphatase, beta-glucuronidase, alpha-mannosidase and hexosaminidase were determined in CSF obtained from patients with proven bacterial meningitis and from patients with various other diagnoses. The mean value for CSF beta-glucuronidase from bacterial meningitis was elevated 73-fold when compared to the aggregate mean of all control groups. Acid phosphatase and alpha-mannosidase means were 26-fold and 33-fold elevated respectively while hexosaminidase was threefold elevated. Measurement of CSF acid phosphatase and beta-glucuronidase should prove a rapid useful test in establishing the diagnosis of bacterial meningitis. Chromatography of CSF samples on DEAE Sephadex allowed the resolution of hexosaminidase and beta-glucuronidase into individual isozymes. The ratio of hexosaminidase A to hexosaminidase B was generally higher in CSF from patients with bacterial meningitis but was very variable. The isozyme distribution for beta-glucuronidase was identical to that found in serum and no differences in pattern were found between patients and control subjects.

Acid Phosphatase↗
PubMed1981

An improved fluorometric leukocyte beta-glucosidase assay for Gaucher's disease.

Three fluorometric leukocyte beta -glucosidase assays were compared for their ability to diagnose Gaucher's disease and identify carriers of the disorder: the acid beta-glucosidase assay of Beutler and Kuhl [2], a pH 5.5-sodium taurocholate-dependent assay and a new procedure which employs conduritol B epoxide, an active-site specific inhibitor of glucocerebrosidase. All three assays unambiguously identified patients with Gaucher's disease. With regard to identifying carriers the bile salt dependent assay of Peters et al. and the conduritol B epoxide-dependent procedure gave the greatest discrimination between the mean beta-glucosidase values for the control and heterozygote samples when evaluated using Student's t test. The most reliable assay for the identification of the carrier state was the conduritol B epoxide-dependent procedure which can be expected to provide the fewest false negative results when classifying heterozygotes (5%). However, the fact that none of these methods will completely separate control and heterozygote samples indicates that their use in screening programs will result in a significant number of incorrect assignments.

Adolescent↗
PubMed1981

Non-A, non-B hepatitis after experimental transmission of malaria by inoculation of blood.

In a 1969 prisoner volunteer study of malaria transmission by blood inoculation, six persons were subinoculated sequentially, and acute hepatitis occurred in the last four (sequential study). Subsequently, another 15 volunteers receiving malaria-rich blood from 14 different donors were followed prospectively (prospective study), and hepatitis developed in six. Incubation periods were shorter but serum transaminase levels were higher for the cases of hepatitis occurring in the sequential study than in the prospective study. Although the illnesses were clinically mild, elevations in transaminase levels persisted for more than six months in five and fluctuating transaminase activities were observed in nine of the 10 affected persons. In addition, an 11th prisoner developed sporadic hepatitis. Neither known human hepatitis viruses nor malaria could be implicated in these cases, which were classified as non-A, non-B (NANB) hepatitis. The data suggested that the viremia of short-incubation NANB hepatitis may begin within the first week after inoculation, confirmed that NANB hepatitis may be transmitted either percutaneously or nonpercutaneously, and provided further evidence that there is more than one NANB agent.

Adult↗
PubMed1981

Evaluation of a new latex agglutination inhibition card test for determining serum gentamicin levels.

A new latex agglutination inhibition card test was compared with a microbiological method and radioimmunoassay for determining serum gentamicin levels. When 60 sera, each containing a known concentration of gentamicin, were analyzed by the microbiological method and by radioimmunoassay, both methods were found to yield accurate and precise results. Analysis of the same 60 sera with the latex agglutination inhibition card test revealed significant variability over a wide range of serum gentamicin concentrations.

Bacillus↗
PubMed1981

Gaucher's disease. I. Modern enzymatic and anatomic methods of diagnosis.

The physician who diagnoses Gaucher's disease should take advantage of the noninvasive method that analyzes WBCs for residual beta-glucocerebrosidase and beta-glucosidase activities. When this method is carried out in conjunction with the measurement of serum acid phosphatase levels, a bone marrow examination may be unnecessary. With this method, we studied an adult who had mild splenomegaly and abdominal pain. When bone marrow was finally obtained subsequent to diagnosis by the enzymatic analysis, the deposits that are specifically formed in Gaucher's disease were easily demonstrated by electron microscopy. We believe that these methods are more specific for the diagnosis of Gaucher's disease than is the light microscopic finding of bone marrow cells that have abundant and striated cytoplasm.

Acid Phosphatase↗
PubMed1980

A revised fluorometric assay for Gaucher's disease using conduritol-beta-epoxide with liver as the source of Beta-glucosidase.

To date, enzymatic diagnosis of Gaucher's disease via a fluorometric assay procedure which utilizes 4-methylumbelliferyl-beta-D-glucopyranoside as a substrate has not been possible when liver serves as the source of enzyme since currently employed fluorometric procedures cannot adequately differentiate between a broad-specificity beta-glucosidase and lysosomal glucocerebrosidase activities in crude extracts of liver. Incorporation of conduritol-beta-epoxide into the incubation medium for the fluorometric assay allows one to selectively measure the glucocerebrosidase activity present in a given liver extract. In five cases of Gaucher's disease this revised fluorometric procedure proved as effective as the assy procedure which utilizes authentic, radiolabeled glucocerebroside as the substrate in demonstrating a deficiency of glucocerebrosidase activity in liver.

Clinical Enzyme Tests↗
PubMed1980

Tuboovarian abscess following laparoscopic sterilization with silicone rubber bands.

Laparoscopic tubal sterilization utilizing Silastic rings generally has been free of infection except when technical complications occur during the procedure. The development of a unilateral tuboovarian abscess in 2 women who had undergone uncomplicated laparoscopic tubal interruption 1 month earlier is reported. In both patients, the pathogens cultured from abscess and/or blood were common members of vaginal-cervical flora, suggesting the infection occurred after the procedure via the ascending route.

Abscess↗