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Biomedical subjects

R Gross

Publications and source records attributed to R Gross.

At least 469 records · Page 26Linked to original sources
PubMed1975

[Chemotherapy of cancer: the present status (author's transl)].

Especially in the treatment of solid tumors, chemotherapy with single agents has so far not been very successful. The principles and results of polychemotherapy are therefore discussed. The experimental and clinical data available suggest that there is no correlation between the number of substances combined and the therapeutic effects. The results of "blind" polychemotherapy are compared with the author's and coworkers own experimental and clinical results with partial synchronization of the tumor cells. If possible the tumor-cell kinetics are determined by a double-labeling method devised by Hilscher and Maurer. In both the experimental field (literature and our group's own examinations) and in clinical experience (still limited), partial synchronization has proved clearly superior to "blind" polychemotherapy.

Animals↗
PubMed1975

[Nitroblue-tetrazolium test in isolated human monocytes].

Depending upon the NBT charge 50 to 80% of isolated non stimulated human monocytes reduce NBT ro morphologically demonstrable Formazan. Similar to neutrophils two different patterns of reaction are exhibited: discrete load with finely distributed Formazan granula surrounding the nucleus; massive reaction with large distributed Formazan granula surrounding the nucleus; massive reaction with large Formazan deposits throughout the cytoplasma. In a low percentage morphologically desintegrated, massive loaden cells appear. After an additional incubation in medium without NBT the number of these necrotic cells increases. The phagocytosis of IgG-coated red cells is impaired after incubation of the monocytes in NBT. In the presence of an alkylating agent not only the NBT-reaction is inhibited; the number of necrotic cells is also diminished. Apparently the Formazan is cytotoxic. The significance of these results in particular in cases of enhanced NBT reduction is discussed. Compared with the findings of other authors concerning the neutrophils the monocytes possess a markedly stronger NBT reducing activity. Considering the lower bactericidal capacity of the monocytes NBT reduction does not in every case parallel bactericidal activity.

Antineoplastic Agents↗
PubMed1975

Reutilization of DNA catabolites in granulocytopoiesis.

Different DNA labelling procedures for the proliferating granulocyte precursor compartments of rat bone marrow were evaluated by studying the appearance of labelled granulocytes in the blood stream by means of autoradiography. 3-H-thymidine was administered by single injection and continuous infusion. 125-I-Iododeoxyuridine, a DNA precursor showing a neglegible extent of reutilization, was studied in comparison. Labelling patterns of blood neurtrophils were identical after single injection and continuous infusion of 3-H-thymidine, while a different pattern was observed after use of a single injection of 125-I-Iododeoxyuridine. The results provide evidence that reutilization of label is an important event to be considered when kinetics of granulocytopoiesis are studied after application of 3-H-thymidine and indicate that reutilization occurs at the level of thymidinemonophosphate in this cell system.

Animals↗