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Biomedical subjects

R Gossrau

Publications and source records attributed to R Gossrau.

At least 163 records · Page 9Linked to original sources
PubMed1976

[Azoindoxyl methods for the histochemical investigation of hydrolases. I. Lactase (lactase-beta-glucosidase complex) (author's transl)].

An azoindozyl method for the histochemical demonstration of lactase (lactase-beta-glucosidase complex) is described. The incubation medium consists of 5 mg 5-Br-4-Cl-3-indolyl-beta-D-fucoside (dissolved in 0.5 ml N,N-dimethylformamide) and 0.02 ml hexazotized prosaniline in 10 ml 0.1 M citric acid phosphate buffer, pH 6-6.5. By means of this method lactase can be exactly localized in the brush border of the enterozytes in the jejunum of suckling rats. Compared to the corresponding indigogenic method the azoindoxyl reaction proceeds faster and the reaction product is often precipitated more precisely.

Animals↗
PubMed1976

Localization of glycoidases with naphthyl substrates.

Unsubstituted naphthyl substrates were found to be superior to substituted naphthyl, indolyl and hydroxyquinoline substrates for the histochemical demonstration of alpha-mannosidase, alpha-galactosidase, hetero-beta-glycosidase, glucoamylase and sucraseisomaltase, equivalent for beta-N-acetylglucosaminidase and lactase-beta-glucosidase, and inferior for beta-glucuronidase and acid beta-galatosidase. Aldehyde fixation is necessary for the localization of lysosomal glycosidases with naphthyl substrates. 1-naphthyl substrates are suitable for the detection of acid glycosidases in lysosomes and hetero-beta-glysocidase in the cytoplasm of animal cells, and 2-naphthyl substrates can be employed for the demonstration of microvillous glycosidases and for the evaluation of the total activity of soluble glycosidases with semipermeable membranes. When naphthyl substrates are used coupling should be carried out simultaneously and hexazotized pararosaniline is the coupling reagent of choice.

Animals↗
PubMed1976

[Histochemistry of amino acid naphthylamidases].

For the correct intracellular localization of amino acid naphthyl-amidases freeze-dried cryostat sections or aldehyde fixed tissue blocks followed by washing in buffer or sugar solution are suitable. Among various amino acid 2-naphthylamides and diazonium salts L-Leucin-4-methoxynaphthylamide and Fast Blue B "pure" (Serva) respectively can be recommended for the histochemical demonstration of these enzymes. In situ the intestinal and renal brush border represent the only cellular structure which actually contain naphthylamidases. By means of alpha,-L-glutamic acid 2-naphthylamide as substrate the glomerula of the kidney also react indicating that further binding sites must exist for amino acid naphthylamidases.

Aminopeptidases↗