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Biomedical subjects

R Gaetje

Publications and source records attributed to R Gaetje.

25 records · Page 2Linked to original sources
PubMed1994

IGF-I and EGF influence on steroid secretion and morphology of human granulosa cells of IVF-cycles and natural cycles in vitro.

The effect of IGF-I, EGF, PDGF and FGF on human granulosa cells of IVF-cycles, and the effect of IGF-I on granulosa cells of natural cycles (day 7 to 13 of cycle) were evaluated in vitro with and without hCG stimulation. At concentrations of 1 ng and 5 ng per ml culture FGF and PDGF did not alter progesterone and estradiol secretion or the morphology of preovulatory granulosa cells. At 1 ng, 2 ng, and 3 ng per ml medium EGF significantly enhanced basal progesterone and estradiol secretion and significantly decreased hCG stimulated estradiol production of preovulatory granulosa cells. While IGF-I treatment with and without hCG stimulation did not alter steroid secretion of preovulatory granulosa cells, the progesterone secretion of granulosa cells of natural cycles was increased by combined treatment with 10 I.U. hCG + 25 ng IGF-I per ml culture medium. Increased steroid secretion was related to reduced cell spreading. Our results provide evidence for the facultative role of IGF-I and EGF as an autocrine/paracrine modulator of ovarian function. IGF-I may play a role in regulation of ovulation induction and luteinisation as IGF-I and hCG act synergistically in increasing progesterone secretion of granulosa cells of natural cycles in vitro and are known to stimulate each other's receptor expression.

Cell Division↗
PubMed1993

Accuracy of bimanual palpation versus vaginosonography in determination of the measurements of pelvic tumors.

In a prospective study the size of 157 pelvic tumors in 101 patients was measured preoperatively by bimanual palpation and vaginosonography, and the results were compared with measurements made at operation. In 72 uterine and 85 ovarian lesions the Spearman correlation coefficients (rs) between the three maximum diameters at right angles to each other measured at operation and by bimanual palpation ranged from 0.48 to 0.78. The correlation coefficients (rs) between measurements at operation and preoperative vaginosonography ranged from 0.91 to 0.97. The size of small lesions was overestimated by bimanual palpation; 44% of tumors smaller than 5 cm in diameter were only detected by vaginosonography.

Diagnosis, Differential↗
PubMed1993

Myometrial biopsy in the diagnosis of adenomyosis uteri.

OBJECTIVE: Automatic cutting needle sampling was used for diagnosis of adenomyosis uteri. The purpose of this study was the establishment of myometrial biopsy as a clinically applicable method. STUDY DESIGN: Sensitivity and specificity of myometrial biopsy in diagnosis of uterine adenomyosis was estimated by examining 680 biopsy specimens from 68 surgically removed uteri. In 34 patients with clinical symptoms of adenomyosis uteri 70 myometrial biopsies were performed during laparoscopy, and vaginal ultrasonographically guided myometrial biopsy was performed in six patients (14 specimens) without complications. Prevention of myometrial bleeding was achieved by prophylactic injection of ornipressin into the biopsy cannula. RESULTS: The sensitivity of a single myometrial sample for diagnosing adenomyosis uteri ranged from 8% to 18.7%; specificity was 100%. CONCLUSION: Our results may encourage further investigation on the clinical use of myometrial biopsy.

Biopsy, Needle↗
PubMed1993

A rabbit model for the evaluation of minimal access treatment of ectopic pregnancy in humans, using intrachorionic injection and local hyperthermia.

Rabbits at mid pregnancy were used as experimental models for investigating the feasibility of intrachorionic injection therapy in human ectopic pregnancy. Also, exposure of rabbit pregnancy to hyperthermia of 45 degrees C was studied. The experiments were followed by serial serum progesterone levels and by light as well as electron microscopy. Intrachorionic injection of different drugs led to demise in 92% of gestations. Application of hyperthermia to the pregnant rabbit uterus led in 94% to demise of gestations. Impairment of the reproductive functions after exposure did not occur. Injection was more effective up to day 15 of pregnancy, while hyperthermia had better results in older gestations. Morphological reactions to injection of different drugs and to hyperthermia were similar. Irreversible damage to the uterine wall was not observed. Intrachorionic injection of drugs may be a suitable treatment of human ectopic pregnancy. Application of hyperthermia may be a promising future therapy.

Abortifacient Agents↗
PubMedDate not supplied

Ovarian cancer antigen CA 125 enhances the invasiveness of the endometriotic cell line EEC 145.

OBJECTIVE: Ovarian cancer antigen CA 125 is used widely for diagnostic and therapeutic evaluation of ovarian cancer. Although the initial description of CA 125 was in 1981, its function is still unknown. In collagen gel system the invasiveness of the endometriotic cell line EEC 145 is enhanced by a proteinaceous factor in peritoneal fluid mimicking molecular features of CA 125. Therefore we hypothesize that this factor is CA 125. METHODS: The influence of heat-treated peritoneal fluid on the invasiveness of the endometriotic cell line EEC 145 and several human carcinoma cell lines (EJ28, RT112, AN3 CA, RL95-2, HEC-1-A, HeLa, MCF7, T-47D, and SK-OV-3) was investigated in a collagen gel invasion assay by addition of (1); 10% heat-treated peritoneal fluid, (2); peritoneal fluid after immunoprecipitation with antibodies directed to CA 125, and (3); purified CA 125. RESULTS: The invasion index of the endometriotic cell line EEC 145 significantly increased from 4.78 +/- 0.98 to 6.57 +/- 1.58 (P = .0001) by addition of 10% heat-treated peritoneal fluid to the culture medium. The invasion-promoting effect of peritoneal fluid was abolished by reduction of the CA 125 concentration through immunoprecipitation and was mimicked in a dose-dependent manner by addition of CA 125 to the culture medium. The invasiveness of the investigated human carcinoma cell lines was not affected by heat-treated peritoneal fluid of CA 125. CONCLUSION: The results are consistent with the hypothesis that CA 125 can influence invasiveness in a benign endometriotic cell line.

Ascitic Fluid↗
PubMedDate not supplied

Tracing cellular and molecular mechanisms involved in endometriosis.

The aetiology and pathogenesis of endometriosis, defined as the presence of endometrium-like tissue outside the uterine cavity, is largely unknown. In this paper we present and discuss possibilities to study the putative pathogenic properties of endometriotic cells in vitro. The current focus of our investigations is on the invasive phenotype of the disease, assuming that this might contribute to the pathogenesis of endometriosis. So far, we have shown that: (i) cytokeratin-positive and E-cadherin-negative endometriotic cells have an invasive phenotype in a collagen invasion assay in vitro similar to metastatic carcinoma cells; (ii) the invasiveness of endometriotic but not of eutopic endometrial cells can be stimulated by a heat-stable protein present in peritoneal fluid; and (iii) the endometriotic cell line EEC145T, which we established, may be a useful tool for the identification of gene products which are, positively or negatively, invasion-related. Finally, our studies suggest that the invasive phenotype in endometriosis shares aspects with tumour metastasis, but might also have unique mechanisms.

Ascitic Fluid↗