Professor Hermann Lehmann: a personal tribute.
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Biomedical subjects
Publications and source records attributed to R G Huntsman.
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A crude extract of outdated, washed, frozen and thawed, platelet concentrates was prepared and optimally diluted for use in the A.P.T.T. test as a source of platelet phospholipid (P.F. 3). The extract contained, in addition to P.F. 3, active heparin neutralizing activity (P.F. 4), which was able to neutralize plasma heparin up to a concentration of approximately 0.8 units/ml plasma. Although these observations are of a preliminary nature, it is felt that the extract can be used to follow the "intrinsic" coagulability of plasma samples from patients receiving therapeutic heparin. Utilizing the A.P.T.T. test system, the determination of coagulability and the neturalization of heparin result from the use of this extract in a single test.
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Statistical and serological evidence from a large kindred and two unrelated adults indicates that Targett (Tar) is an antigen in the Rh blood group system and that its presence is assocciated with a weak expression of the Rh antigen D. In the numerical notation the Tar antigen is designated Rh40.
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In two patients with severe painful sickle cell crisis and a deteriorating clinical course, recovery coincided with large-scale exchange transfusions (6 liters). The one patient, with sickle cell beta thalassemia, achieved a 90% exchange, calculated on the sickle cell hemoglobin present before and after transfusion. The other patient, with sickle cell hemoglobin Hb A, S, and (Hb) C disease, also achieved an 80% exchange calculated on the level of HbA.S. AND C in samples before and after transfusion.
The characterisation of haemoglobin G Norfolk (alpha2 85 Asp leads to Asn 2) is described. This variant has been identified in the heterozygous state in three apparently unrelated English families of which two are resident in England and the other in Canada.
Eight patients with beta thalassaemia major suffering from leg ulcers, were treated over an 8-week period with 3 g ascorbic acid daily in a controlled double-blind crossover study. The ulcers of all the patients showed a high rate of either complete or partial healing.
161 inhabitants of the Seychelle Islands have been examined for blood group, serum protein, and red cell enzyme polymorphisms. The gene frequency data obtained from this survey supports the anthropological view that the present-day Creole-speaking inhabitants of the Seychelle Islands result from a admixture of African and European stock.
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Haemoglobin A(2) in 11 normal and 42 B thalassaemia minor subjects was estimated by simple visual assessment of the electrophoretogram. The results obtained give rise to doubts as to the reliability of this technique when used to estimate haemoglobin A(2) under normal laboratory conditions.
An automated alkali denaturation technique which measures fetal haemoglobin is described. This method offers greater speed and a lower standard deviation than comparable manual methods.
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