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Biomedical subjects

R G Hess

Publications and source records attributed to R G Hess.

54 records · Page 3Linked to original sources
PubMed1986

[Infectious diarrheal diseases in swine: morphologic and microbiologic findings].

Infectious diarrhea in piglets can be caused by TGE, EVD and rota virus. The present report shows another viral agent (para-rota virus) to be present. In addition the report describes light and electronmicroscopic findings of the enteric mucosa caused by the agents mentioned. The degree and distribution of villous atrophy, one of the main histological findings, of infections with TGE, EVD, rota and para-rota virus is compared. A correlation of the results obtained with fluorescent antibody staining and ultrastructural diagnosis of agents was done. An application of the latter seems advisable in case of negative FA staining results.

Animals↗
PubMed1985

Percutaneous transluminal coronary angioplasty after intracoronary streptokinase in evolving acute myocardial infarction.

To achieve optimal myocardial revascularization and prevent rethrombosis of the infarct-related coronary artery, percutaneous transluminal coronary angioplasty (PTCA) was attempted in 18 patients with evolving acute myocardial infarction (9 anterior and 9 inferior) after administration of intracoronary streptokinase. PTCA was attempted 338 +/- 151 minutes after the onset of symptoms. After thrombolytic therapy, 11 patients had a severe residual stenosis and 7 a persistent total occlusion of the infarct-related coronary artery. PTCA was successful in 13 of 18 patients: in 9 of 11 with coronary stenoses and in 4 of 7 with total coronary occlusions. PTCA reduced the severity of the coronary lesion from 91 +/- 2% to 27 +/- 7% (p less than 0.001), and the transstenotic pressure gradient from 38 +/- 5 to 6 +/- 2 mm Hg (p less than 0.01). One patient in cardiogenic shock died during urgent coronary surgery after unsuccessful PTCA. After PTCA, all patients received heparin and antiplatelet agents. One patient had reinfarction with reocclusion of the infarct-related artery 5 days after PTCA. The other 12 patients had an uneventful hospital course, and cardiac catheterization before hospital discharge (8 to 17 days) revealed reocclusion of the infarct-related coronary artery in 3 and persistent patency in 9. Persistent patency of the infarct-related artery was associated with preservation of left ventricular end-diastolic volume (initial 86 +/- 6 ml/m2, follow-up 91 +/- 6 ml/m2), and improvement in left ventricular ejection fraction in some patients.

Adult↗
PubMed1984

Rotavirus-specific antibodies in fetal bovine serum and commercial preparations of serum albumin.

Rotavirus-specific antibodies were detected in fetal bovine serum, bovine serum albumin, and human serum albumin by radioimmunoprecipitation with the NCDV strain of bovine rotavirus as the detecting antigen. Fetal bovine sera neutralized bovine rotavirus in a plaque reduction neutralization test to titers of 1:20 or greater. Immunoglobulins purified from fetal bovine serum by protein A-agarose affinity chromatography precipitated rotavirus antigens but did not neutralize bovine rotavirus. Rotavirus antibodies in fetal bovine serum and in purified serum albumin preparations may interfere with diagnostic assays for the detection of rotavirus antigens or antibodies.

Animals↗
PubMed1983

Local immunity in rotaviral infections.

After a short introduction into the mechanisms of nonspecific defence in the intestine, a brief review on specific, local intestinal immunity is given. Results of experiments on local and humoral antibody production carried out in five caesarian-derived, colostrum-deprived calves are presented. In each of the calves, two jejunal fistulas were prepared, and samples from fistulas and faeces were taken for antigen and antibody determinations at intervals for up to 120 days post infection. Antibodies in the jejunum appeared between 3 and 12 days and in faeces 4 to 18 days post infection. Antibodies could be detected in the faeces for 25 to 58 days. Reinfection of these calves after 50 to 70 days p. inf. usually resulted in a secondary response; however, excretion of the virus was not observed after reinfection.

Animals↗
PubMed1981

Distribution of antibodies to rotavirus in serum and lacteal secretions of naturally infected swine and their suckling pigs.

Rotavirus antibodies were demonstrated in lacteal secretions and sera of 20 parturient sows and in sera of their newborn by an enzyme-linked immunosorbent assay blocking technique, using bovine rotavirus cell culture antigen and monospecific antibody to bovine rotavirus. Antibodies to rotavirus occur in the 3 immunoglobulin (Ig) classes IgM, IgA, and IgG in lacteal secretions. High and long-persisting antibody activity was mainly associated with the IgA class. The IgM and IgG decreased to undetectable concentrations in most sows during the 14-day investigation period. Serum antibodies of newborn pigs nursing their dams also decreased rapidly during this time. The heterologous enzyme-linked immunosorbent assay blocking technique was a reliable and rapid procedure for the demonstration of rotavirus antibodies.

Animals↗
PubMed1981

Routine isolation and cultivation of bovine rotaviruses in cell culture.

Using the bovine embryonic kidney cell line, Aubek, bovine rotaviruses were routinely isolated from fecal samples of calves with diarrhea. Of 125 fecal samples positive for rotavirus by immune electron microscopy and the enzyme-linked immunosorbent assay, 61 isolates were recovered and cultivated continuously.

Animals↗
PubMed1976

In vitro differentiation and pH sensitivity of field and cell culture-attentuated strains of transmissible gastroenteritis virus.

Characteristics of four transmissible gastroenteritis (TGE) virus field strains (Miller, Purdue, Bl, and V203) and four cell culture-attenuated strains (Purdue, SH, CKp, and Bl) were studied to find methods of differentiation between the two groups of viruses. TGE field virus strains did not replicate as well as attenuated strains at 37 C and could not be passaged serially for more than four to six passages at 33 C. There were clear differences in plaque size when the strains were compared. Field strains had average plaque sizes ranging from 3.59 to 3.15 mm, whereas attenuated strains induced plaques that were larger than 4.2 mm. Variations were observed in stability of strains at pH 3.0. Field strains and cell culture-attenuated strains CKp-270 and SH-114 were reduced in titer by about 1 log10. A reduction of about 3 log10, however, was obtained with cell culture strains B1-300 and Purdue-113.

Cell Line↗
PubMedDate not supplied

Measuring nursing governance.

BACKGROUND: Despite outcomes studies measuring such variables as job satisfaction, autonomy, professionalism, turnover, leadership styles, and more recently, cost-effectiveness, no consistent relationships have been established between shared governance models and outcomes. OBJECTIVES: To define and develop an instrument to measure the governance of hospital-based nurses. METHODS: The 88-item Index of Professional Nursing Governance (IPNG) was developed to measure professional nursing governance of hospital-based nurses. Psychometric properties were tested with 1,162 registered nurses from 10 hospitals. RESULTS: Content validity after item generation was .95, using Popham's average congruency procedure. Six factors explained 42% of the variance with subscale intercorrelations between .43 and .67. All subscales had a high degree of internal consistency (alphas .87 to .91); test-retest reliability was .77. Construct validity testing showed that scores between shared governance and traditionally governed hospitals were significantly different. A correlation was found between scores on the IPNG and the Hague and Aiken Index of Centralization. CONCLUSIONS: The results of this study support the validity of the 88-item IPNG as a reliable instrument for measuring the distribution of professional nursing governance of hospitals.

Adult↗
PubMedDate not supplied

Shared governance: innovation or imitation?

Is nursing shared governance an innovation or merely an imitation of other organizational governances? A simple determination is confused by the euphemisms applied to similar governance forms and the simultaneous occurrence of related concepts like participation and decentralization. In the absence of a common model, anecdotal literature suggests that the implementation of shared governance in specific hospitals contributes to further innovation in governance.

Decision Making, Organizational↗