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Biomedical subjects

R G Graw

Publications and source records attributed to R G Graw.

At least 73 records · Page 4Linked to original sources
PubMedDate not supplied

Occurrence of granulocyte cytotoxins and agglutinins.

Granulocyte cytotoxic activity in sera from over 257 patients was shown to be distinct from HL-A lymphocytoxic activity. Granulocyte cytotoxins occur in approximately 25 per cent of sera from patients having leukemia, 45 per cent with aplastic anemia, 22 per cent with kidney disease on hemodialysis, and 19 per cent of pregnant women. By testing sera on the same panel of cells, the granulocyte cytotoxic activity was shown not to be associated with granulocyte agglutination activity or lymphocytotoxic acitivty. It is likely that granulocyte cytotoxins and granulocyte agglutinins will be useful in transfusion and bone marrow transplantation as a separate tool from the more widely used lymphocyte cytotoxicity reaction.

Agglutination Tests↗
PubMedDate not supplied

Consequences of prior alloimmunization during granulocyte transfusion.

The transfusion of leukocyte-containing blood products can lead to the production of antibodies to antigens on the surface of leukocytes. Such antibodies can be detected by a variety of techniques including assays for lymphocytotoxicity, granulocytotoxicity, and leukoagglutination. In order to evaluate the effect of preformed anti-leukocyte antibodies during granulocyte transfusion therapy, recipient beagles were sensitized to donor foxhound antigens. After being made granulocytopenic with cyclophosphamide, these animals were transfused with a set dose of granulocytes collected by continuous flow centrifugation. When compared to the results of similar transfusions to nonsensitized recipients, granulocyte transfusions to animals with preformed anti-leukocyte antibodies resulted in lower one-hour posttransfusion leukocyte increments (p less than .04) and in less migration of neutrophils through a skin abrasion into a chamber containing a strong chemoattractant, autologous serum (p less than .0001). Also, profound thrombocytopenia was found in sensitized animals, but not in nonsensitized recipients, one hour after the granulocyte transfusion.

Animals↗
PubMedDate not supplied

The relationship between donor-recipient lymphocytotoxicity and the transfusion response using HLA-matched platelet concentrates.

Matching donor-recipient pairs for HLA antigens provides a logical starting point for selecting platelet donors for thrombocytopenic recipients who have demonstrated refractoriness to pooled random donor platelet transfusions. However, not all recipients achieve a compatible transfusion response with platelets selected by HLA type, indicating that additional selection methods are required. Because of studies indicating that a positive lymphocyte cross-match assay between recipient serum (antibody) and donor lymphocytes predict acute renal allograft rejection, it was hoped that this assay might be useful in predicting the platelet transfusion response for alloimmunized patients. The data herein indicate that the results of this assay in no way correlate with the platelet transfusion response. The lymphocyte cross-match would seem to be helpful in the selection of donors where known HLA antigen mismatches occur between donor and recipient. However, even in this situation, no correlation was observed between the transfusion response and donor-recipient lymphocytotoxicity reaction. Clearly additional assays are needed for the selection of compatible donors from those matched by HLA antigens.

Blood Donors↗