Search PubMed⌕ Search

Biomedical subjects

R Fries

Publications and source records attributed to R Fries.

At least 181 records · Page 10Linked to original sources
PubMed1988

Chromosomal localization of the major histocompatibility complex of the horse (ELA) by in situ hybridization.

The first gene assignment to a horse chromosome is reported for equine leucocyte antigen (ELA), the major histocompatibility complex of the horse. A cloned DNA sequence derived from a class I gene of the porcine major histocompatibility complex was used as a probe for an in situ hybridization experiment. We present the regional localization of ELA, using this sequence, to equine chromosome 20q14-q22.

Animals↗
PubMed1988

The loci for parathyroid hormone and beta-globin are closely linked and map to chromosome 15 in cattle.

We report linkage of the loci for beta-globin (HBB) and parathyroid hormone (PTH) in cattle and the assignment of both loci to the bovine chromosome region 15q13-q23. Linkage was analyzed in a family of paternal half-sibs by the use of restriction fragment length polymorphisms detected with bovine probes derived from the HBB and PTH genes. The HBB polymorphism was detected by digestion with restriction endonuclease HindIII and the PTH polymorphism with MspI. The maximum lod score for linkage of PTH with HBB was zeta = 4.52 at theta = 0, suggesting very close linkage of the two loci. The finding of the PTH/HBB linkage is corroborated by the physical assignment of both loci to the region 15q13-q23 by in situ hybridization with bovine genomic probes derived from PTH and HBB, respectively. Since HBB and PTH are syntenic in man and mouse, these results in cattle represent another example of conservation of synteny in the evolution of mammalian chromosomes.

Alleles↗
PubMed1988

Porcine malignant hyperthermia carrier detection and chromosomal assignment using a linked probe.

In pigs, the gene for glucosephosphate isomerase (GPI) is linked to the halothane (HAL) gene which is responsible for malignant hyperthermia (MH). A single copy DNA probe, designated GPI8R, has been isolated from a pig genomic library using a porcine GPI cDNA probe. This probe detects, as was the case for the cDNA probe, a five allele polymorphism in SacI and PvuII digested pig DNA. Family studies show that this polymorphism is linked to the HAL locus and hence can be used in carrier detection. In situ hybridization with GPI8R assigned the GPI locus to bands p12-q22 of chromosome 6. We conclude that the HAL linkage group resides on chromosome 6.

Animals↗
PubMed1987

Qualitative radiographic diagnosis of osteo-arthritis of the knee joint in the C57BL mouse.

Experiments were performed to assess the diagnostic value of radiography in osteo-arthritis (OA) of the knee joint in C57Bl mice. Comparative histological and radiographic examination of knee joints from 96 animals showed that if the classical diagnostic criteria (narrowing of the joint space, sclerosis of the subchondral bone, deformation of the joint epiphyses) are applied, OA is not readily detectable by radiography. If these criteria are extended to include radiographic changes in the menisci (enlargement, deformation, increased and/or heterogeneous density), the frequency of detection is much improved. However, except in the severest forms of OA, radiography does not afford a satisfactory means of distinguishing between various degrees of severity. The qualitatively evaluated data nevertheless do suggest that quantitative radiographic diagnosis of OA of the murine knee joint may be useful, both for improving the detection rate and for discriminating various degrees of OA.

Animals↗
PubMed1987

[Process control in large-scale technical meat production--poultry].

1. The technical process of poultry meat production influences the quantitative and qualitative bacteriological status of the food broiler-meat in a characteristic and especially explicable manner. 2. Successful provisions to improve the bacteriological status of deep-frozen broiler-meat have to be enforced in the earlier stages of poultry meat production. 3. Controlling the bacteriological status of a product by recourse to the gaining technology is a bearing concept as for self-control-aspects as for official supervision. 4. Nevertheless, to guarantee the comparison of data, a reproducible and as simple as possible bacteriological technique is necessary, which can be commonly accepted and which covers a broad spectrum of bacteria.

Animals↗
PubMed1986

Mapping genes in domesticated animals.

Gene maps are constructed by the synthesis of data obtained by different methods which include family analyses, somatic cell hybridization, direct mapping of DNA segments by Southern blot analysis, and in situ hybridization to fixed metaphase chromosomes. Gene mapping has already contributed significantly to a better understanding of the mammalian genome, in particular the human genome, but the gene maps of economically important domestic species are not well-characterized. The application of somatic cell genetics and recombinant DNA methodologies now allows rapid progress to be made in the construction of detailed gene maps for domestic animals. Such gene maps will serve as tools for selection in applied animal breeding and for the analysis of polygenic traits.

Animals↗