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Biomedical subjects

R Freund

Publications and source records attributed to R Freund.

71 records · Page 4Linked to original sources
PubMed1981

[Importance of the preparation for the double contrast examination of the colon (author's transl)].

In a consecutive series of 299 routine double-contrast studies of the colon, X-ray films were examined prospectively for remaining stool in the colon. In 47%, the radiological diagnosis was restricted by stool. There was no statistic significant difference in patient's sex and age, and also none in the abnormal radiological findings compared to the group with good cleaning. The results elucidate important steps in preparing the patient for a clean colon: (1) the necessity of the radiologist being active in the preparation. (2) Written instructions and explanations must be given to every patient. (3) Regular motivation of the physicians and nursing teams is essential. Only a clean colon ensures early detection of colorectal polyps and efficient prevention of colorectal cancer.

Colon↗
PubMed1980

Four heat shock proteins of Drosophila melanogaster coded within a 12-kilobase region in chromosome subdivision 67B.

Unique coding sequences for four heat shock proteins of Drosophila melanogaster, hsp 28, hsp 26, hsp 23, and hsp 22, are clustered in a 12-kilobase interval at chromosome subdivision 67B. The four genes are not transcribed in the same direction and each gives rise to a separate messenger RNA, with no indication of intervening sequences. Including the present results, the genes for all seven major heat shock proteins of D. melanogaster are now cloned are found to exhibit a variety of patterns of organization at the five loci they occupy.

Animals↗
PubMed1980

[A new class of highly active progestagens: 17 alpha-CH2X-substituted gona-4,9(10)-dienes. 58. Steroids].

The synthesis of new gona-4,9(10)-dienes with a 17 alpha-CH2X-substituent (X = CN, N3, Cl or Br) is described. The progestagenic activity of these substances was studied in the McPhail assay using immature rabbits. The compound XVI (X = CN, STS 557) is the most potent one, showing an activity about ten times higher than D-norgestrel. Differences in the activity caused by different routes of application are discussed.

Animals↗
PubMed1979

Studies of cloned sequences from four Drosophila heat shock loci.

DNA cloned from the D. melanogaster (Oregon R) heat shock loci at 63BC and 95D codes for the 83,000 and the 68,000 dalton heat shock proteins, respectively. Both coding sequences occur once per haploid genome. Sequences complementary to messenger RNA for the 70,000 dalton heat shock protein are represented five times, twice at 87A and three times at 87 C. The copies at 87A differ characteristically from those at 87C in an interval of a few hundred bp near the 5' end of the messenger sequence, and the corresponding two classes of hsp 70 messenger RNA are found on polysomes after heat shock. Within this differential region, there is about 15% divergence between messenger sequences cloned from the two loci, while in the rest of the messenger region examined the homology is much closer although still imperfect. Unexpectedly, considerable homology is found between the sequence for the 68,000 dalton heat shock protein at 95D and the sequences for the 70,000 dalton protein at 87A and 87C, and between these sequences and a site in 87D. Messenger RNA molecules of 2.4, 2.55 and 3.05 kb code for the 68,000, 70,000 and 83,000 dalton heat shock proteins and hybridize to apparently uninterrupted DNA sequences of 2.1, 2.25 and 2.6 kb, respectively.

Animals↗
PubMed1978

Sequence organization and transcription at two heat shock loci in Drosophila.

The heat shock loci of Drosophila melanogaster chromosome subdivisions 87A and 87C have been studied by using cloned DNA. Both sites contain a number of copies of a 2,4-kilobase (kb) region homologous to mRNA for the 70,000-dalton heat shock protein. In situ hybridization to chromosomal RNA shows that transcripts of this sequence accumulate at both sites after temperature elevation. At 87C there is a 1.5-kb repeated sequence homologous to another heat shock RNA. One cloned segment includes two to three tandem copies of this sequence located 0.8 kb from the beginning of a 2.4-kb message region. RNA complementary to the 1.5-kb repeat acccumulates at 87C after temperature elevation, but does not code for any known heat shock protein. In the sibling species D. simulans, there are sequences located and transcribed at 87A and 87C that are homologous to the melanogaster 2.4-kb message sequence. The 1.5-kb repeat, however, is absent from 87C in simulans and no heat shock RNA homologous to it can be detected.

Animals↗