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Biomedical subjects

R Fontanges

Publications and source records attributed to R Fontanges.

At least 19 recordsLinked to original sources
PubMed1979

[Importance of respiratory route in experimental salmonellosis in Balb/c mice].

Infection of Mice with S. typhimurium, was conducted by respiratory, oral and peritoneal routes. The results obtained showed that infection occurred more quickly by i. p. than by other routes. However i. n. inoculation is the more efficient concerning Bacteria concentration per Mousse to obtain the DL 100. A study of Salmonella growth in vivo was conducted in lungs, spleen, liver and blood. The results are discussed in relation to the calf respiratory Salmonellosis actually observed.

Animals↗
PubMed1979

[Enzymatic activity of peritoneal macrophages of Balb C mice after enteral or parenteral stimulation with bacterial extracts].

A study of peritoneal macrophage activation has been conducted by simultaneous investigation in eleven enzymes in Balb/c Mice stimulated with bacterial extracts, by oral and parenteral route. The results showed important changes in Macrophage number and activity. On the other hand this activation depended upon the route of administration, antigen nature, and time interval between stimulation and peritoneal harvesting.

Animals↗
PubMed1979

Role of interferon in mice in protection against influenza A virus by bacterial ribosomes together with membranal glycoproteins of Klebsiella pneumoniae as adjuvant.

Nonspecific protection against infectious aerosols of influenza A virus was obtained in Swiss mice after vaccination by aerosols of bacterial ribosomes together with membranal glycoproteins extracted from Klebsiella pneumoniae as the adjuvant. It was shown that repeated stimulant aerosols were necessary to obtain this protection. Routine estimation of serum interferon levels after administration of the association of ribosomes plus membranal glycoproteins to the animals by aerosol or intravenous route showed that there was no correlation between protection and the presence of serum interferon. It was shown that the serum interferon-inducer activity was due to ribosomes. No induction of serum interferon was obtained with membranal glycoproteins used separately. Local liberation of interferon in the mucous membrane of the upper respiratory tract was not investigated.

Adjuvants, Immunologic↗
PubMed1979

[Effect of anti-inflammatory agent administration on Balb/c mouse peritoneal macrophage activity].

A study of the mouse Balb/c peritoneal macrophages has been made after oral antiinflammatory drugs administration on animals which previously had an inflammation with carrageenan. The results show an enhancement of the number of the macrophages, of their enzymatic activities and of their proteins' content. These three parameters fall down more or less rapidly according to the considered antiinflammatory drug.

Acid Phosphatase↗
PubMed1979

Multiplication of normal and virus-infected diploid cells cultivated on microcarriers.

In prelminary studies, growth of diploid cells (fetal calf buccal cells and chick embryo fibroblasts on dextran microcarriers was studied and compared to growth in monolayer. Growth coefficients obtained were always greater (by 2 fold) when microcarriers were used. Moreover, this technique had the advantage of reducing the number of manipulations and hence the risk of contamination. In two series of experiments, buccal epithelial cells were infected with vescicular stomatitis virus. Good virus multiplication was obtained.

Animals↗
PubMed1979

Endocrine interaction of the thymus with the hypohysis, adrenals and testes: effects of two thymic extracts.

The concentration of ACTH, corticosterone, LH and testosterone in the plasma of rats have been measured in normal, thymectomized and sham-operated rats. A group of rats, thymectomized at birth, received two i.p. injections weekly of two thymic extracts, each prepared in a separate way. Neonatal thymectomy was followed by the appearance of a transitory wasting disease during which a hormonal unbalance was manifest. Plasma corticosterone and ACTH levels increased at first at 30 days after thymectomy and then decreased at 60 days. The levels of the different hormones returned to normal by 90 days. The injections of thymic extracts rectified all these perturbations. The plasma levels of testosterone and corticosterone of hypophysectomized rats were compared to those of hypophysectomized and thymectomized animals. Results suggested that the thymus has a direct action of the adrenal cortex and in indirect action on the testes. The indirect effect is probably mediated through the hypophysis.

Adrenal Glands↗
PubMed1979

[Studies in the BALB/c mouse of the efficiency of spraying as compared to other inoculation ways for immunostimulant (author's transl)].

The of efficiency of spraying as compared to intranasal and digestive ways was tested on Balb/c mouse by using on the one hand an immunostimulant product and on the other various types of test : Myxovirus influenzae by aerosol, Streptococcus pyogenes intranasally, Klebsiella pneumoniae intraperitoneally. Results showed that in the case of influenza virus the immunostimulant inhaled as liquid aerosol or powder aerosol, or deposited on the nasal mucosa was very active. When the test germ was Streptococcus and the contamination intra-nasal, only the homologous way was efficient. When the test germ was Klebsiella pneumonia all inoculation forms except powder aerosol were effective. The comparative study of aerosols granulometry helps grasping this phenomenon.

Aerosols↗
PubMed1979

["In vitro" interactions between influenza virus and mouse lung alveolar macrophages (author's transl)].

Interactions between influenza virus A/PR/8/34 (H0N1) and Balb/c mouse lung alveolar macrophages have been studied in vitro. One day after initiation of alveolar macrophage culture in 35 mm Falcon dishes, the virus suspension was allowed to adsorb to the cells for 1 h. Detachment of cells from the plastic substrate, morphological changes in adherent cells and decreased phagocytosis of heat-killed Candida albicans occured slowly as compared to control cultures. These facts appeared to be directly correlated to the concentration of viruses in the inoculum. Data yielded by virus titrations, electron microscopy and immunofluorescence suggest that mouse lung alveolar macrophages are able to take up a large amount of viral particles and inhibit their replication, allowing only an abortive viral cycle.

Animals↗
PubMed1979

[Morphometric study of the bronchopulmonary lymphoid system in the normal mouse and the mouse infected with influenza virus].

Morphometric study of mouse bronchus-associated lymphoid tissue (BALT) using an image analyser and histological sections at close and regular intervals was carried out in lungs of control and influenza infected mice two to three months old. The mean area of BALT did not reach 0,1 p. 100 of the whole mean area of examined lung sections in control mice; this percentage was significantly greater one month after primary exposure to influenza virus.

Animals↗
PubMed1978

[Antitetanus vaccination: preliminary results of a study in Africa].

Two commercialized anatoxins, titrating 30 Lf per dose and adsorbed on Ca phosphate and Al hydroxide respectively, were studied in comparison with an experimental anatoxin titrating 25 Lf per dose and adsorbed on Al phosphate, and with a placebo. 595 schoolchildren from Cameroun were randomly assigned to four groups and inoculated twice, with a year's interval between the two inoculations. Serological checks were carried out by double-blind trials using the passive hemagglutination (HA) method on days 7, 90, 365 and 395. HA titers rose substantially after three months and then fell during the 12th month. The HA method does not give a clear indication of the amount of protection obtained after only one inoculation, but this amount would seem to be small according to the results of neutralization tests carried out on samples. It is only after the second injection that protection is provided for most subjects. Results vary depending on the anatoxin and are not linked to the--benign--clinical reactions observed. This study confirms that if anatoxins with high antigenicity are to be used, two injections are necessary and sufficient to guarantee good immunity protection.

Adolescent↗