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R Facius

Publications and source records attributed to R Facius.

At least 19 recordsLinked to original sources
PubMed2003

Critical issues in connection with human missions to Mars: protection of and from the Martian environment.

Human missions to Mars are planned to happen within this century. Activities associated therewith will interact with the environment of Mars in two reciprocal ways: (i) the mission needs to be protected from the natural environmental elements that can be harmful to human health, the equipment or to their operations; (ii) the specific natural environment of Mars should be protected so that it retains its value for scientific and other purposes. The following environmental elements need to be considered in order to protect humans and the equipment on the planetary surface: (i) cosmic ionizing radiation, (ii) solar particle events; (iii) solar ultraviolet radiation; (iv) reduced gravity; (v) thin atmosphere; (vi) extremes in temperatures and their fluctuations; and (vii) surface dust. In order to protect the planetary environment, the requirements for planetary protection as adopted by COSPAR for lander missions need to be revised in view of human presence on the planet. Landers carrying equipment for exobiological investigations require special consideration to reduce contamination by terrestrial microorganisms and organic matter to the greatest feasible extent. Records of human activities on the planet's surface should be maintained in sufficient detail that future scientific experimenters can determine whether environmental modifications have resulted from explorations.

Aerospace Medicine↗
PubMed2002

Investigation of radiation doses in open space using TLD detectors.

The low energy component of the cosmic radiation field is strongly modified by the shielding of the spacecraft and it is time and location dependent. Thermoluminescent lithium fluoride detectors have been applied to determine the radiation doses inside the ESA-Facility BIOPAN. The BIOPAN facility was mounted outside and launched on a Foton spacecraft and opened to space to allow exposure of several experiments to open space. Standard TLD-600. TLD-700 chips, two layers MTS-Ns sintered pellets with different effective thickness of the sensitive layer and MTS-N of different thickness have been exposed with different shielding thicknesses in front of them. The measured TL signal in the 0.1 mm thick detector just shielded by an aluminised Kapton foil of 25 microm thickness in front yielded a dose of 29.8 Gy (calibrated with 137Cs gamma rays) for an exposure time of 12.7 days: after 2.5 g.cm(-2) shielding the doses dropped to 3 mGy. The monitoring of radiation doses and its depth dose distribution outside the spacecraft are of great interest for radiation protection of astronauts working in open space. The knowledge of depth-dose distribution is a prerequisite to determine the organ doses an astronaut will receive during an extravehicular activity (EVA). The BIOPAN experiments are to be continued in the future.

Aerospace Medicine↗
PubMed2001

Exposure of arctic field scientists to ultraviolet radiation evaluated using personal dosimeters.

During July 2000 we used an electronic personal dosimeter (X-2000) and a biological dosimeter (Deutsches Zentrum für Luft- und Raumfahrt: Biofilm) to characterize the UV radiation exposure of arctic field scientists involved in biological and geological fieldwork. These personnel were working at the Haughton impact structure on Devon Island (75 degrees N) in the Canadian High Arctic under a 24 h photoperiod. During a typical day of field activities under a clear sky, the total daily erythemally weighted exposure, as measured by electronic dosimetry, was up to 5.8 standard erythemal dose (SED). Overcast skies (typically 7-8 okta of stratus) reduced exposures by a mean of 54%. We estimate that during a month of field activity in July a typical field scientist at this latitude could potentially receive approximately 80 SED to the face. Because of body movements the upper body was exposed to a UV regimen that often changed on second-to-second time-scales as assessed by electronic dosimetry. Over a typical 10 min period on vehicle traverse, we found that erythemal exposure could vary to up to 87% of the mean exposure. Time-integrated exposures showed that the type of outdoor field activities in the treeless expanse of the polar desert had little effect on the exposure received. Although absolute exposure changed in accordance with the time of day, the exposure ratio (dose received over horizontal dose) did not vary much over the day. Under clear skies the mean exposure ratio was 0.35 +/- 0.12 for individual activities at different times of the day assessed using electronic dosimetry. Biological dosimetry showed that the occupation was important in determining daily exposures. In our study, scientists in the field received an approximately two-fold higher dose than individuals, such as medics and computer scientists, who spent the majority of their time in tents.

Adult↗
PubMed2000

Systematic study of parameters influencing the action of Rose Bengal with visible light on bacterial cells: comparison between the biological effect and singlet-oxygen production.

As part of a project to study different methods for the disinfection of effluent water, the inactivation of different microorganisms (Escherichia coli, Deinococcus radiodurans and spores of Bacillus subtilis) using a combination of a photosensitizer (Rose Bengal) with simulated sunlight and oxygen was determined under various environmental conditions (temperature, pH index). In parallel, the singlet-oxygen (1O2) production was also measured under the same conditions. Whereas the vegetative cells could be inactivated much more efficiently at increased temperature and altered index of pH, the production of 1O2 remained essentially the same under these alterations. Additionally, the relations among the sensitivities of different cell types to be killed by our photodynamic treatments (PDT) were opposite to those found after exposure to ionizing radiation. The results of photodynamic experiments do not reflect the cells' capacity to repair DNA strand breaks. Spores of B. subtilis, as a nonvegetative system, could not be inactivated by illuminations up to 100 J cm-2. Together, these findings indicate that DNA is not the primary target, the inactivation of which leads to the killing of our test organisms. Instead, the cellular envelope appears to be the component being assaulted by our PDT.

Bacteria↗
PubMed1999

Manned missions to Mars and chromosome damage.

PURPOSE: To estimate the frequencies of dicentric chromosomes in peripheral lymphocytes of astronauts on missions to Mars. MATERIALS AND METHODS: Calculations of expected frequencies of dicentrics are based on dose estimations and lymphocyte biology. RESULTS: Frequencies of dicentrics are predicted to be 10-40 times higher than the background level. CONCLUSIONS: Stressing the importance of careful mission planning and optimized shielding of transfer vehicle to Mars.

Astronauts↗
PubMed1997

Chromosomal aberrations in blood lymphocytes of astronauts after long-term space flights.

PURPOSE: To analyse lymphocyte chromosomes from astronauts of MIR and EUROMIR missions. MATERIALS AND METHODS: Peripheral lymphocytes from seven astronauts before and after space flights were cultured and analysed for structural chromosomal aberrations. RESULTS: Chromosome-type but not chromatid-type aberrations were significantly elevated after space flights when compared to pre-flight values. In one astronaut two rogue cells were found. CONCLUSIONS: Overall the frequencies of aberrations were found to be correlated with estimated absorbed cosmic radiation doses.

Astronauts↗
PubMed1996

Biological dosimetry of solar radiation for different simulated ozone column thicknesses.

During the Spacelab mission D-2, in the experiment RD-UVRAD, precalibrated biofilms consisting of dry monolayers of immobilised spores of Bacillus subtilis (strain Marburg) were exposed, for defined intervals, to extraterrestrial solar radiation filtered through an optical filtering system, to simulate different ozone column thicknesses. After the mission, the biofilms were processed and optical densities indicative of any biological activity were determined for each exposure condition by image analysis. For the different simulated ozone column thicknesses, biologically effective irradiances were experimentally determined from the biofilm data and compared with calculated data using a radiative transfer model and the known biofilm action spectrum. The data show a strong increase in biologically effective solar UV irradiance with decreasing (simulated) ozone concentrations. The full spectrum of extraterrestrial solar radiation leads to an increment of the biologically effective irradiance by nearly three orders of magnitude compared with the solar spectrum at the surface of the Earth for average total ozone columns.

Bacillus subtilis↗
PubMed1995

Results of space experiments.

Life science research in space was started in Europe with the first Biostack experiment flown onboard Apollo 16 in 1972. Biostack was designed to investigate the biological effects of single heavy ions of cosmic radiation. Among several undertakings towards this goal, the Biostack achieved the highest precision in the determination of the spatial correlation of the observed biological response of single test organisms to the passage of single heavy ions, which is the mandatory requirement. It also provided information on the influence of additional spaceflight factors, such as microgravity, on radiation effects and measurements of the spectrum of charge and energy of the cosmic radiation. The experiment was performed as an international cooperation effort. This report gives a summary of the biological data accumulated in this and the follow-on experiments of the Biostack program.

Animals↗
PubMed1994

Particle trajectories in seeds of Lactuca sativa and chromosome aberrations after exposure to cosmic heavy ions on Cosmos Biosatellites 8 and 9.

The potentially specific importance of the heavy ions of the galactic cosmic radiation for radiation protection in manned spaceflight continues to stimulate in situ, i.e., spaceflight experiments to investigate their radiobiological properties. Chromosome aberrations as an expression of a direct assault on the genome are of particular interest in view of cancerogenesis being the primary radiation risk for man in space. In such investigations the establishment of the geometrical correlation between heavy ions' trajectories and the location of radiation sensitive biological substructures is an essential task. The overall qualitative and quantitative precision achieved for the identification of particle trajectories in the order of approximately 10 micrometers as well as the contributing sources of uncertainties are discussed. We describe how this was achieved for seeds of Lactuca sativa as biological test organisms, whose location and orientation had to be derived from contact photographies displaying their outlines and those of the holder plates only. The incidence of chromosome aberrations in cells exposed during the COSMOS 1887 (Biosatellite 8) and the COSMOS 2044 (Biosatellite 9) mission was determined for seeds hit by cosmic heavy ions. In those seeds the incidence of both single and multiple chromosome aberrations was enhanced. The results of the Biosatellite 9 experiment, however, are confounded by spaceflight effects unrelated to the passage of heavy ions.

Chromosome Aberrations↗
PubMed1994

Inactivation of individual Bacillus subtilis spores in dependence on their distance to single cosmic heavy ions.

For radiobiological experiments in space, designed to investigate biological effects of the heavy ions of the cosmic radiation field, a mandatory requirement is the possibility to spatially correlate the observed biological response of individual test organisms to the passage of single heavy ions. Among several undertakings towards this goal, the BIOSTACK experiments in the Apollo missions achieved the highest precision and therefore the most detailed information on this question. Spores of Bacillus subtilis as a highly radiation resistant and microscopically small test organism yielded these quantitative results. This paper will focus on experimental and procedural details, which must be included for an interpretation and a discussion of these findings in comparison to control experiments with accelerated heavy ions.

Bacillus subtilis↗
PubMed1994

Inactivation of individual Bacillus subtilis spores in dependence on their distance to single accelerated heavy ions.

In order to understand radiation mechanisms of heavy ions in detail, it is necessary to study effects of single ions on individual biological test objects. Spores of Bacillus subtilis have been used as a suitable small biological test system to measure the inactivation in dependence on the radial distance to the tracks of charged particles. Accelerator experiments have been performed using a modified Biostack technique--biological objects sandwiched between nuclear track detectors. Results of these experiments using ions differing in their energy and atomic number will be discussed under following aspects: (i) methodological differences between the experiments and their possible influences on the results, (ii) common features which are independent on the particle type and energy, (iii) theoretical expectations and problems to find solid theoretical concepts which explain the results.

Argon↗
PubMed1990

Dosimetry results of COSMOS 1887.

The objective of the experiment was to measure the radiation environment inside and outside of the biosatetlite COSMOS 1887. For this purpose, detector packages were built up consisting of plastic detectors and nuclear emulsions having different linear energy transfer (LET) thresholds in particle registration, and thermoluminescence dosimeters (TLD). Particle fluence rates, LET-spectra and absorbed dose are presented. Absorbed dose is measured as a function of shielding depth. The data are compared with those of other missions.

Animals↗
PubMed1990

Reliability of trajectory identification for cosmic heavy ions and cytogenetic effects of their passage through plant seeds.

The potentially specific importance of the study of heavy ions from galactic cosmic rays for the understanding of radiation protection in manned spaceflight continues to stimulate spaceflight experiments in order to investigate the radiobiological properties of these ions. Chromosome aberrations as an expression of a direct assault on the genome are of particular interest in view of carcinogenesis as the primary radiation risk for man in space. An essential technical ingredient of such spaceflight experiments is the visual nuclear track detector which permits identification of those biological test organisms which have been affected by cosmic heavy ions. We describe such a technique and report on an analysis of the qualitative and quantitative reliability of this identification of particle trajectories in layers of biological test organisms. The incidence of chromosome aberrations in cells of lettuce seeds, Lactuca sativa, exposed during the Kosmos 1887 mission, was determined for seeds hit by cosmic heavy ions. In those seeds the incidence of both single and multiple chromosome aberrations was enhanced.

Chromosome Aberrations↗
PubMed1989

Cell inactivation, repair and mutation induction in bacteria after heavy ion exposure: results from experiments at accelerators and in space.

To understand the mechanisms of accelerated heavy ions on biological matter, the responses of spores of B. subtilis to this structured high LET radiation was investigated applying two different approaches. 1) By the use of the Biostack concept, the inactivation probability as a function of radial distance to single particles' trajectory (i.e. impact parameter) was determined in space experiments as well as at accelerators using low fluences of heavy ions. It was found that spores can survive even a central hit and that the effective range of inactivation extends far beyond impact parameters where inactivation by delta-ray dose would be effective. Concerning the space experiment, the inactivation cross section exceeds those from comparable accelerator experiments by roughly a factor of 20. 2) From fluence effect curves, cross sections for inactivation and mutation induction, and the efficiency of repair processes were determined. They are influenced by the ions characteristics in a complex manner. According to dependence on LET, at least 3 LET ranges can be differentiated: A low LET range (app. < 200 keV/micrometers), where cross sections for inactivation and mutation induction follow a common curve for different ions and where repair processes are effective; an intermediate LET range of the so-called saturation cross section with negligible mutagenic and repair efficiency; and a high LET range (>1000 keV/micrometers) where the biological endpoints are majorly dependent on atomic mass and energy of the ion under consideration.

Bacillus subtilis↗
PubMed1989

Influence of cosmic radiation and/or microgravity on development of Carausius morosus.

Eggs of Carausius morosus were exposed to spaceflight conditions in two spaceflight missions, the German 7 day Spacelab Mission D1 and the Soviet 12.56 day Biosatellite Mission "COSMOS 1887". During spaceflight the eggs continued their development. Eggs of five different ages representing different sensitivity to radiation and different capacity to regeneration were used to investigate the influence of cosmic radiation and/or microgravity on insect development. Using the Biostack concept--eggs in monolayers sandwiched between nuclear track detectors--and the 1 g reference centrifuge of BIORACK in D1 we were able to separate effects of heavy ions of the cosmic radiation from microgravity effects and also from combined effects of these two factors in space. After retrieval, hatching rates, embryonic and larval growth kinetics and anomaly frequencies were determined. Microgravity leads to a reduced hatching rate of eggs exposed in the early stages of development. Hatching was normal in eggs which were exposed on the 1 g reference centrifuge. Hits by heavy ions caused body anomalies. The combined action of heavy ions and microgravity resulted in an unexpectedly high frequency of anomalies. These results obtained from the Spacelab Mission D1, were confirmed in an experiment onboard of COSMOS 1887. In addition to the previous analysis, embryonic development before hatching was followed which showed no major difference between flight and the ground control specimens. Since a reconfirmation of reduced hatching rates was observed in COSMOS 1887, too, the above results suggest some microgravity induced functional impairment of the hatching activity, rather than blockage in embryonic development.

Abnormalities, Radiation-Induced↗
PubMed1988

Radiation problems in manned spaceflight with a view towards the Space Station.

With the advent of a permanently manned Space Station, the longstanding problems of radiation protection in manned spaceflight have acquired an immediacy. This paper endeavors to emphasize the gaps of our knowledge which must be closed for effective radiation protection. The information that is required includes the accurate determination of the exposure inside the space station to the various components of the ionizing radiation, the evaluation of the biological importance of the different radiation qualities and the depth-dose distribution of the less penetrating component. There is also the possibility of an interaction with weightlessness. It is necessary to establish adequate radiation protection standards and a system of dosimetric surveillance. There is a need for studies of methods on the possibilities of hardening selective shielding of the space station. Spaceflight experiments, which might contribute to the solution of some of these problems are discussed.

Atlantic Ocean↗
PubMed1986

Mutation induction in spores of Bacillus subtilis by accelerated very heavy ions.

Mutation induction (resistance to sodium azide) in spores of Bacillus subtilis was investigated after irradiation with heavy ions from Neon to Uranium with specific particle energies between 0.17 and 18.6 MeV/u. A strong dependence of the mutation induction cross section on particle charge and energy was observed. From the results it was concluded that mutation induction in bacterial spores by very heavy ions is mainly caused by secondary electrons.

Bacillus subtilis↗