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Biomedical subjects

R F Ritchie

Publications and source records attributed to R F Ritchie.

At least 55 records · Page 3Linked to original sources
PubMed1977

Improved electroimmunoassay of factor VIII-related antigen.

Factor VIII-related antigen migrates poorly into gel during electrophoresis in agarose, probably in major part because of the relatively high sulfate content of the commonly used agars. A recently available low-sulfate residue agar, substituted for standard agar preparations in electroimmunoassay, allows Factor VIII antigen to travel more rapidly, producing "rockets" that are well defined and easily measured, making the assay easier and more reliable.

Antigens↗
PubMed1976

Immunofixation. III. Application to the study of monoclonal proteins.

We have shown how immunofixation can be successfully used to study proteins exhibiting electrophoretic polymorphism. An analogous situation is seen in the disorders of the immune system that result in the production of variable quantitites of homogeneous immunoglobulins. Immunoelectrophroesis has been heavily used--sometimes unsuccessfully because of poor resolution--to study these materials. The appearance of a monoclonal immunoglobulin band is identical in both immunofixation and standard gel electrophoresis, a feature that allows unambiguous identification of a protein as to its heavy-chain class and light-chain type. Because antigen and antibody are placed in contact almost immediately after electrophoresis, interfering diffusion does not occur and even extremely small bands can be demonstrated and characterized.

Antibodies, Anti-Idiotypic↗
PubMed1976

Immunofixation. I. General principles and application to agarose gel electrophoresis.

Immunofixation offers the worker an economical means of physically locating a protein in an electrophoretic strip and is ideally suited to forensic medicine, genetic studies, or research. The method is as simple and economical as the commonly used one- or two-dimensional immunoelectrophoresis, yet yields considerably more information.

Blood Protein Electrophoresis↗