Effects of releasing sterile male Japanese beetles on the fertility of ova of an isolated population in eastern Tennessee.
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Biomedical subjects
Publications and source records attributed to R F Harris.
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The adenosine triphosphate content of Selenastrum capricornutum was maintained within 1.4 to 3.4 mug of adenosine triphosphate/mg (dry weight) of living biomass at different growth stages in media of different phosphorus concentrations.
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Maintenance of substrate water potential in petri dishes is achieved by using vapor-pressure controlling, solute-amended agar gel discs attached to the inside of the top halves of the dishes.
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The plate-dilution frequency technique described facilitates simultaneous enumeration of a wide range of physiologically different microorganisms in complex systems with a precision comparable to dilution tube (most probable number) methods. Replicate microsamples are inoculated from each member of a dilution series onto areas delineated on plates of prepoured solid media; the plates are incubated, and the occurrence of growth or specific biochemical transformation is recorded for each inoculated area. Microbial enumeration is accomplished by reference to appropriate tables. Details of the experimental procedures are described, and tables are presented from which microbial numbers with 95% confidence limits can be obtained and compared for significant difference directly for 10-fold and 4-fold dilution series. Results of experiments in which microbial populations were estimated simultaneously by the plate-dilution frequency and conventional plate count methods are compared. The potential of the technique for broad-spectrum microbial assay is also discussed.
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