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Biomedical subjects

R F Cross

Publications and source records attributed to R F Cross.

47 records · Page 3Linked to original sources

Autolytic changes in the digestive system of germfree, escherichia coli monocontaminated, and conventional baby pigs.

Tissues from three groups of pigs (germfree, Escherichia coli monocontaminated, and healthy conventional pigs) were collected at intervals between 24 minutes and 7 hours 12 minutes after death. Histological differences between the three groups were present in the alimentary mucosa and were most striking in the ileum and colon.Autolytic change was detected only in the digestive system and the sequence of autolytic events was similar in all groups. The time of onset and rate of progress differed markedly between groups and between segments of the alimentary canal. In the conventional group, autolysis started quickly and progressed rapidly. In the germfree group it was later in onset and slower in progress. The E. coli infected group was intermediate. The initial visible change occurred in the small intestine. The morphological differences and autolytic changes were described.

Animals↗

Studies of escherichia coli in gnotobiotic pigs. VI. Effects of feeding bacteria-free filtrates of broth cultures.

Nine gnotobiotic pigs derived from one gilt were fed bacteria-free filtrates prepared from: 1) cultures of an enteropathogenic strain of Escherichia coli 09:K.:NM (Strain 340), 2) cultures of a nonenteropathogenic strain of E. coli 08.K..H16 (Strain CDC-1466-56), and 3) uninoculated culture medium. Diarrhea was observed initially two to four hours after feeding the filtrate prepared from the enteropathogenic E. coli. The duration of diarrhea was five to ten hours. No diarrhea was observed after feeding filtrate prepared from uninoculated medium or cultures of nonenteropathogenic E. coli. The pH values of the feces increased with the onset of diarrhea and decreased to normal after diarrhea stopped. No histopathological lesions were found.

Animals↗

A buffy coat stimulating effect in serum from cattle with persistent lymphocytosis.

A stimulating effect on bovine buffy coat cells was demonstrated in blood samples from cattle with persistent lymphocytosis. The stimulation was a function of the serum and not the cells used. It was not altered by filtration or inactivation of the serum. When buffy coat cells were grown in homologous serum, the resulting growth was moderate to abundant when the donor animal had persistent lymphocytosis and was usually poor when the donor animal had a consistently normal hemogram.

Animals↗