Search PubMed⌕ Search

Biomedical subjects

R E Thompson

Publications and source records attributed to R E Thompson.

At least 73 records · Page 4Linked to original sources
PubMed1985

Adenosine deaminase binding protein, a new diagnostic marker for kidney disease.

This enzyme immunoassay detects adenosine deaminase binding protein (ABP), a glycoprotein that is shed from the brush border of the proximal tubule in kidney damage. Two monoclonal antibodies, URO-4 and URO-4a, each react with different epitopes on ABP and are used as the "sandwich" pair of antibodies. A linear standard curve can be generated by using partly purified ABP isolated from the urine of patients with kidney disease. Release of ABP into the urine appears to reflect the severity of the insult to the nephron. Therefore, measurement of ABP in urine may help distinguish between tubular disease and glomerular disease and indicate renal allograft rejection in renal-transplant patients.

Antibodies, Monoclonal↗
PubMed1985

Competitive idiotype--anti-idiotype immunoassay for adenosine deaminase binding protein in urine.

This competitive immunoassay, based on inhibition by antigen of the idiotype-anti-idiotype interaction, detects adenosine deaminase binding protein (ABP), and involves use of monoclonal anti-idiotype antibodies prepared to a monoclonal antibody specific for ABP. The conditions for this new type of competitive immunoassay are investigated. This competitive immunoassay is as sensitive and reproducible as an earlier described "sandwich"-type immunoassay for ABP (Clin Chem 31: 679-683, 1985). Evaluation of urine samples from normal subjects and from patients showed increased concentrations of ABP in patients with renal disease.

Antibodies, Anti-Idiotypic↗
PubMed1983

Characterization studies of glucose dehydrogenase.

Porcine liver beta-D-glucose dehydrogenase has been isolated using Triton X-114 to release it from the endoplasmic reticulum. The purified enzyme contains a limited amount (1.7%) of lipid material, including cholesterol, fatty acids, mono and diglycerides, phosphatidylcholine, phosphatidylethanolamine, and cholesterol esters. This enzyme is a tetrameric protein containing an extensive number of hydrophobic residues. This form of glucose dehydrogenase is capable of turning over both beta-D-glucose and alpha-D-glucose-6-phosphate in vivo as indicated from a steady state kinetic analysis at 37 degrees C.

Amino Acids↗
PubMed1983

Primary substrate specificity determinants for the H4-specific protease-activated protein phosphotransferase.

The specificity of the histone-H4-specific, protease-activated protein kinase (H4-PK) was examined using two series of synthetic peptides corresponding to the phosphorylation sites in histone H4 and pyruvate kinase. Optimum kinetic constants for phosphorylation were observed using the peptide Val-Lys-Arg-Ile-Ser-Gly-Leu. Peptides in which the Lys was replaced by Arg or the Lys-Arg sequence was transposed were phosphorylated with less favorable kinetics. Peptides with either basic residue deleted did not serve as substrates. Only the H4 peptide, containing an Arg-Arg sequence, was phosphorylated by the cyclic-AMP-dependent protein kinase (CA-PK). Distinct specificity determinants for H4-PK and CA-PK were also observed using the pyruvate kinase peptide (Leu-Arg-Arg-Ala-Ser-Leu-Gly). Collectively the data indicated that the primary substrate specificity determinants for H4-PK are Lys-Arg-Xaa-Ser whereas the CA-PK selectively phosphorylates the sequence Arg-Arg-Xaa-Ser.

Amino Acid Sequence↗
PubMed1983

Guanidinium- and temperature-induced conformational changes in glucose dehydrogenase.

The conformational changes in glucose dehydrogenase are studied as a function of temperature and guanidinium chloride (GdmCl) concentration. The data were analyzed assuming a two-conformer model which gave similar results using either circular dichroism or enzyme activity. The free energy of denaturation was 0.94 kcal/mol from specific activity and 1.64 kcal/mol from circular dichroism measurements. The mid-point of the denaturation curve was 0.65 or 0.63 M GdmCl, as determined by specific activity or circular dichroism, respectively. The transition temperature, 6.4 degrees C, is close to that of a microsomal membrane phase change, a result that is consistent with the fact that glucose dehydrogenase contains lipid materials when isolated with a non-ionic detergent such as Triton X-114. As the temperature increased, the amount of beta-pleated sheet increased, and the alpha-helical content decreased, suggested that glucose dehydrogenase contains a stable core of beta-pleated sheet.

Animals↗
PubMed1983

The pendulum swings.

Explore the source record for details and available documents.

Economic Competition↗
PubMed1983

Characterization of lecithin:cholesterol acyltransferase from human plasma: II. Physical properties of the enzyme.

The physical properties of purified human plasma lecithin:cholesterol acyltransferase (LCAT) were investigated by techniques including analytical ultracentrifugation, ultraviolet spectroscopy, electrofocusing, and circular dichroism. The partial specific volume of LCAT was determined by sedimentation equilibrium ultracentrifugation experiments in H2O and D2O solutions (0.702 ml/g). The Mr was 67,000 by sodium dodecyl sulfate (SDS)-gel electrophoresis and 60,000 by sedimentation equilibrium ultracentrifugation. The discrepancy between the two sets of data presumably arose from the glycoprotein nature of the enzyme. Studies of the ultraviolet spectrum indicated that LCAT contained 6.5% (w/w) tyrosine which corresponds to approximately 18 tyrosine residues/mol of LCAT (polypeptide Mr 45,000). Spectrophotometric titration of the ionizable phenolic side chains indicated that nearly all the tyrosine residues were buried at neutral pH while they became gradually exposed at higher pH. The apparent pK of this transition was about 12.0 contrasted with 9.8, the apparent pK of ionization of the free tyrosyl groups.

Centrifugation, Density Gradient↗
PubMed1983

Close-up picture of TEFRA basics.

The concept of "business as usual" for health care institutions is no longer an acceptable modus operandi. The Tax Equity and Fiscal Responsibility Act of 1982 has forever changed the relationship between medical staff members, governing boards, and administrative staff and made mutual cooperation more important than ever.

Medical Staff, Hospital↗
PubMed1982

Nutritional amblyopia associated with jejunoileal bypass surgery.

After jejunoileal bypass surgery for morbid obesity, metabolic complications including a nutritional amblyopia developed in a patient. Reanastomosis of the bypass and treatment with multivitamins resulted in a nearly complete return of vision. Any patient undergoing rapid weight loss after gastrointestinal surgery for morbid obesity should be followed up for possible decrease in vision. If nutritional amblyopia develops, immediate reversal of the previous surgery is indicated.

Adult↗