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Biomedical subjects

R E Spier

Publications and source records attributed to R E Spier.

90 records · Page 5Linked to original sources
PubMed1980

Variation in the susceptibility of BHK populations and cloned cell lines to three strains of foot-and-mouth disease virus.

BHK monolayer and suspension cell populations maintained in different laboratories were found to vary in their susceptibility to infection with three strains of foot-and-mouth disease virus (FMDV). The susceptibility of parent cell populations was compared with that of individual clones of cells derived from them. The populations tested in this way consisted of a number of cell types, each expressing a different capacity to produce FMDV. The relative numbers of susceptible and insusceptible cells in each population appeared to determine the overall susceptibility of that population. The ability of the FMDV strain Asia 1 Iran 1/73 to multiply was an indicator of the general FMDV susceptibility of a BHK cell population.

Animals↗
PubMed1980

Foot-and-mouth disease virus production in glass sphere based monolayer systems of 0.1 to 100 L medium volume.

The cell and virus productivity of a 3 mm glass sphere based unit process monolayer system was determined at scales of 0.1, 1, 10 and 100 litres medium capacity. Enhanced yields were obtained up to the 10L size when compared with control Roux bottle cultures. Modifications of the operating conditions of the 100L system should bring this into line with the smaller-scale units.

Animals↗
PubMed1979

The use of frozen BHK21 C13 cells to control the biological parameters for cell and foot-and-mouth disease virus growth.

In order to control the four primary variables (cells, serum, medium and physical conditions) in a cell or virus growth system it is important to have a reliable and constant positive control with which to compare any variable of the system components. The use of aliquots of a frozen cell population (stored at -136 degrees C) for this purpose is the subject of this paper. Using such cells, tests have been established for the control of sera, media, cell susceptibility and the quality of serum treated DEAE Sephadex A50 beads.

Animals↗
PubMed1976

The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of glass spheres.

In view of the advantages which are associated with the use of the BHK monolayer cell for the production of foot-and-mouth disease (FMD) virus, a unit system using glass spheres was developed to grow BHK monolayer cells and to test the susceptibility of such cells to FMD virus. The yield of cells and their susceptibility compares favorably with GREEN BHK monolayer cells which have been grown in Roux bottles.

Animals↗
PubMed1976

The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of DEAE sephadex A50 beads.

Methods are described which make possible the production of foot-and-mouth disease (FMD) virus from BHK 21 C13 monolayer cells which have been grown on the surface of serum coated DEAE Sephadex A50 beads. The yield of cells and their susceptibility to infection by FMD virus are equivalent to conventional Roux monolayer systems. The potential for the commercial application of the DEAE Sephadex A50 system is discussed in relation to other unit process monolayer systems and in particular to the system in which cells are cultured in a deep bed of small glass spheres.

Animals↗
PubMed1976

Studies on the susceptibility to foot--and--mouth disease virus of BHK cell cultures derived from various sources.

Cultures of BHK monolayer and suspension cells obtained from a number of laboratories and a group of cloned sub-lines derived from suspension cells were examined for their susceptibility to three FMD virus strains. It was found that the various cultures were sensitive to the test virus strains to differing degrees. It was shown possible to obtain a clone with high susceptibility to Asia 1 Iran 1/73 virus from a parent culture which had a low susceptibility to that virus. The implication of these findings is discussed.

Aphthovirus↗
PubMed1976

Reproducibility of yields of foot--and--mouth disease virus from BHK monolayer and suspension cells.

The production of FMD virus from BHK 21 C13 monolayer and suspension cells was examined under standardized conditions and in different systems. The yields of virus from suspension cells did not significantly exceed the yield from monolayer cells, whereas the monolayer cell was capable of producing virus from some strains of FMD virus which would not grow in suspension cells. When different production systems were examined, the scale of operation did not significantly influence the yield of virus from suspension cells, while the plastic spiral film propagator produced significantly less virus than the other monolayer systems examined. The use of unit monolayer systems on the large scale is discussed.

Aphthovirus↗