Specific effects of blood plasma from beef cows fed pine needles during late pregnancy on increasing tone of caruncular arteries in vitro.
Consumption of Ponderosa pine needles by cows during late pregnancy results in the premature delivery of viable calves. Previous observations made at necropsy suggested that vascular insufficiency in the caruncular bed was associated with this induced parturition. The present studies used the perfused caruncular arterial bed of an isolated bovine placentome to determine whether blood plasma collected from cows fed pine needles contained a vasoactive factor(s). Changes in caruncular arterial tone (i.e., long-term changes in vessel diameter) and phasic contractility (i.e., transient reductions in vessel diameter) were evaluated. Tone was quantified as the pressure exerted against a constant intraluminal flow and as the force of contraction during K(+)-stimulated depolarization of membranes. Phenylephrine, an alpha 1-adrenergic agonist, was used to stimulate a phasic contractile response. Jugular blood was collected from beef cows fed pine needles (n = 4) or a control diet (n = 4), during the last 3 d of gestation, and plasma was used for two studies. In Study 1, placentomes were perfused with decreasing dilutions (1:150 to 1:40) of plasma pooled over the last 3 d of pregnancy from cows fed pine needle or control diets. Caruncular arterial perfusion pressures and responses to K+ progressively increased (P less than .05) with decreasing dilutions of plasma from cows fed pine needles, and responses to phenylephrine remained constant. Caruncular arterial perfusion pressures and responses to K+ and phenylephrine remained constant during perfusion of plasma from cows fed the control diet. In Study 2, the changes in vasoactivity of plasma (1:60 dilution) from cows fed pine needles was evaluated during the last 3 d of pregnancy.(ABSTRACT TRUNCATED AT 250 WORDS)