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Biomedical subjects

R E Kirsch

Publications and source records attributed to R E Kirsch.

156 records · Page 9Linked to original sources
PubMedDate not supplied

Radioimmunoassay of human ligandin.

A sensitive and specific radioimmunoassay for human ligandin has been developed and used to study ligandin release into the serum in acute and chronic hepatitis. Serum ligandin concentrations were elevated in 67 of 68 cases of acute viral hepatitis. Ligandin levels frequently returned to normal within the first 2 weeks of illness. The rapid disappearance of ligandin preceded the return to normal of serum SGOT. In chronic active hepatitis, serum ligandin levels correlated significantly (p less than 0.01) with histologic severity of disease. This correlation was not seen with SGOT. Serum ligandin may be useful in monitoring progress and the need for therapy in chronic active hepatitis.

Aspartate Aminotransferases↗
PubMedDate not supplied

Glutathione S-transferase in human hepatocellular carcinoma.

Qualitative and quantitative changes in glutathione S-transferase (GSH-T) were studied in human hepatocellular carcinoma. GSH-T specific activity (mumoles per min per mg protein) was variably reduced in hepatocellular carcinoma. Similar changes were seen in "cationic" GSH-T (ligandin) concentration determined by radioimmunoassay. Immunohistochemical studies with antihuman liver ligandin suggest that positive staining was more frequently found in well-differentiated tumors. The relative activities of "cationic," "neutral," and "anionic" transferases (pI greater than 7.5) activity ranged from virtually absent to near normal values. "Neutral" (pI 6 to 6.5) and "anionic" (pI less than 5.4) species were present more often in tumors than in normal liver. In two cases, normal liver tissue and tumor were obtained from the same patient. In one, only quantitative differences were present, while in the other "cationic" and "neutral" GSH-Ts were present in the normal liver tissue while both "cationic" and "anionic" species were found in the tumor. Our studies indicate that qualitative as well as quantitative changes of GSH-T occur in human hepatocellular carcinoma.

Biopsy↗
PubMedDate not supplied

Further studies on the ridge at the internal edge of the cataract incision.

A pathologic and experimental study of the etiology and morphology of the ridge, visible gonioscopically in the region of the internal lips of the cataract incision during and after the cataract operation, is described. The ridge observed clinically cannot be reproduced in human eye-banks eyes but can be reproduced in vivo in the eye of the owl monkey. Histopathology and ultramicroscopic studies of these experimental eyes, together with evidence derived from histopathologic slides of postmortem human eyes, suggest that the ridge is probably the result of immediate edematous swelling of the deeper layers of the corneal stroma after the incision transects corneal endothelium and the Descemet membrane. While internal wound lip malapposition may be a contributing factor to formation of the ridge, it is probably not an essential component. The ridge is not caused by the incision-closing sutures for it is already present prior to suturing of the wound. The role in ridge formation of mechanical stress alterations produced immediately by the incision is considered but is not known.

Animals↗
PubMedDate not supplied

Fibrinogen synthesis in the isolated perfused rat liver: stimulation by a humoral factor associated with trauma.

Fibrinogen synthesis was measured by the [14C]carbonate method in isolated rat livers perfused with blood from normal rats and rats injected with turpentine 1, 5, and 16 hr before bleeding. Five- and 16-hr blood increased synthesis 2-fold, compared with normal and 1-hr blood. Turpentine injection caused a 10-fold rise in fibrinogen fragment D immunoreactivity (measured by serum radioimmunoassay) 5 and 16 hr after injection, compared with normal rats, and rats bled 1 hr after turpentine injection. Isolation of fibrinogen-related antigen by immunoaffinity chromatography, followed by polyacrylamide gel electrophoresis, revealed substantial increases in fibrinogen-related protein in 5- and 16-hr blood. The largest relative increase was in protein with electrophoretic mobility similar to that of fragment D1. These studies demonstrate the presence of a transferable blood factor which, produced in response to injury, stimulates fibrinogen synthesis by the liver. They lend support to the possibility that this factor may be fragment D1 or one or more other derivatives of the D domain of the fibrinogen molecule.

Albumins↗