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Biomedical subjects

R E Gordon

Publications and source records attributed to R E Gordon.

At least 163 records · Page 9Linked to original sources
PubMed1978

Some bits and pieces of the genus Nocardia: N. carnea, N. vaccinii, N. transvalensis, N. orientalis and N. aerocolonigenes.

Since 1951 when our taxonomic study of the genus Nocardia began, only eight strains of N. carnea (Rossi-Doria) Castellani & Chalmers, four strains of N. vaccinii Demaree & Smith, five of N. transvalensis Pijper & Pullinger, 21 or N. orientalis (Pittenger & Brigham) Pridham and 14 of N. aerocolonigenes (Shinobu & Kawato) Pridham have been found. These five groups of strains are described and are compared with strains of nine accepted species of Nocardia. With the possible exception of N. orientalis, more strains of these five taxa must be assembled and examined to establish the reliability of our descriptions and identifications.

Culture Media↗
PubMed1977

Electron microscope demonstration of tubulin in cilia and basal bodies of rat tracheal epithelium by the use of an antitubulin antibody.

It has been previously demonstrated that both cytoplasmic microtubules and the microtubules of cilia, flagella, and sperm tail contain tubulin. Although the morphology of cytoplasmic microtubules and that of axonemes differs in cells from which they have been isolated, the tubulin of the two structures shares physical and chemical properties. In some mammalian tissues, such as tracheal epithelium, cilia and basal bodies are difficult to isolate and characterize. The use of an enzyme-labeled immunoglobulin probe would facilitate identification and in situ localization of such proteins. Tubulin prepared from porcine brain by ion-exchange chromatography and from rat brain by the method of cyclic polymerization and depolymerization with subsequent disk gel electrophoresis with SDS were injected intravenously into rabbits. The animals were intermittently bled and the antisera extracted. The specificity of the antisera was proved by indirect immunofluorescence staining of the mitotic spindle, specific blocking of spindle staining by purified tubulin and not by other proteins, staining of 3T3 cytoplasmic microtubules, single line on immunoelectrophoresis, failure of control antisera to show any of these, and precipitation of antibody with all tubulin preparations and not with actin. We have shown by electron microscopy of ciliated cells of the tracheal epithelium stained with antitubulin by the indirect enzyme-labeled antibody method that the basal bodies, outer doublets, and central pair of the cilia contain tubulin. This indicates that tubulin in microtubules of cilia and basal bodies of rat tracheal epithelium is antigenically similar to tubulin extracted from cytoplasmic neurotubules of brains from the same species and from a different mammalian species. No other axonemal structures stained with the antitubulin. Three different preparations of tubulin from pigs and rats were used to immunize rabbits. All elicited similar antisera which gave identical staining patterns. The specificity of the staining was demonstrated by the absence of staining with immune serum absorbed with purified tubulin, the absence of staining with preimmune serum, and the absence of staining if any of the reagents were omitted during the staining reaction.

Animals↗
PubMed1977

Cytokinetics of rat tracheal epithelium stimulated by mechanical trauma.

Mild abrasion of rat tracheal epithelium results in irreversible damage to the superficial cells and stimulates the viable basal cells to participate in a nearly synchronous wave of DNA synthesis and mitosis. For the growth population as a whole, DNA synthesis started at 14 hr after injury and persisted for 16 hr. The duration of S in individual cells was determined autoradiographically by identifying the time at which a second pulse of DNA precursor (14C-TdR) was no longer incorporated by cells labelled with 3H-TdR at the onset of S. S was found to be 8-9 hr long. It was also determined that cells entering S at later times synthesized DNA for the same 8-9 hr period. TG2 was calculated to be 2 1/2-3 1/2 hr by subtraction of TS and 1/2TM from the period from onset of DNA synthesis to metaphase. By making a second denuding lesion adjacent to the first injury, the cells were stimulated through at least another period of S. At the peak of the second wave of DNA synthesis (50 hr after injury) 14C-TdR was present in the same cells which had incorporated 3H-TdR administered at the mid-point of the preceeding synthetic phase. The 28-hr interval between these two peaks of synthesis is the measure of cell cycle duration for these regenerating tracheal epithelial cells.

Animals↗
PubMed1977

Thrombotic thrombocytopenic purpura: report of case.

TTP is an acute, usually fatal disease of unknown etiology. Diagnostic confirmation of the disease requires histologic demonstration of the deposition of hyaline thrombi within the lumens of arterioles and capillaries in the absence of vasculitis. The clinical, laboratory, and histological manifestations of TTP were reviewed and a case was reported in which a gingival biopsy specimen was used to confirm the diagnosis. Emphasis is placed on the rapidity and ease of gingival biopsy and the possibility of earlier institution of therapy.

Adult↗
PubMed1976

Regeneration of rat tracheal epithelium after mechanical injury. II. Restoration of surface integrity during the early hours after injury.

Mechanical injury to ciliated and goblet cells of the rat tracheal epithelium results in sloughing of these cells. The basal cells of the pseudostratified epithelium remain. These cells, studied by scanning electron microscopy, transmission electron microscopy, and en face light microscopy after silver staining, are rounded and separated from one another for the first 2 hours after injury. They become flat and are apposed to each other by 4 hours after injury, but inter-cellular junctions are not uniformly present until 6 hours after injury. By that time, early evidence of stratification of cells is observed, and lanthanum hydroxide, as a colloidal tracer placed in the tracheal lumen, is excluded from the spaces between cells. The changes are indicative of rapid restoration of barrier function after mild injury to the respiratory tract lining as a first step in the reparatve process.

Animals↗
PubMed1974

The Baker Act can work.

Explore the source record for details and available documents.

Community Mental Health Services↗
PubMed1973

Polysaccharides of the genus Bacillus cross-reactive with the capsular polysaccharides of Diplococcus pneumoniae type 3, Haemophilus influenzae type b, and Neisseria meningitidis group A.

We studied 174 strains of the genus Bacillus for cross-reacting antigens to the capsular polysaccharides of groups A and C meningococcus, types I and III pneumococcus, and Haemophilus influenzae type b. Cross-reactions were detected by immunodiffusion in agarose gel by using type-specific antisera and confirmed by absorption and inhibition experiments. Of 20 Bacillus pumilis strains, six had an antigen cross-reacting with group A meningococcal polysaccharide. Other cross-reactions included one strain of B. pumilis with H. influenzae type b, one of B. cereus var. mycoides with pneumococcus type III, and one of B. alvei with both type b and SIII polysaccharides. These cross-reacting antigens are polysaccharides of vegetative cells and may be extracellular in location. Because these bacilli have antigens cross-reacting with the virulence factors of pyogenic bacteria, they may, as normal flora, be an antigenic stimulus for "natural" serum anti-capsular antibodies to the type b Haemophilus and group A meningococcus polysaccharides.

Antigens, Bacterial↗