Search PubMed⌕ Search

Biomedical subjects

R E Davis

Publications and source records attributed to R E Davis.

At least 289 records · Page 16Linked to original sources

An automated method for the microbiological assay of serum pyridoxal.

A fully automated method for the measurement of serum pyridoxal has been developed. Acid phosphatase was used for dephosphorylation and precipitation of the serum proteins was not required. A chloramphenicol-resistant strain of L. casei was used as the test organism and this removed the need for sterilization. The method gives highly reproducible results, and is suitable for population and institutional studies.

Acid Phosphatase↗

An automated microbiological method for the measurement of vitamin B12.

An automated method for the microbiological assay of vitamin B(12) is described. A chloramphenicol-resistant strain of Lactobacillus leichmannii is used as the test organism and this eliminates the need for sterilization or aseptic addition. Precipitation of the serum protein is avoided by dilution of the serum with a glutamic/malic acid solution before heating the preparation to free the vitamin B(12). Tests can be set up at a rate of 80 an hour and after incubation they can be read at 160 an hour. By interfacing a programmed electronic calculator directly with the Mecolab M which is used for the assays, results are printed directly in ng/l.

Autoanalysis↗