The heat stability of synthetic and natural pyridoxal.
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Biomedical subjects
Publications and source records attributed to R E Davis.
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A fully automated method for the measurement of serum pyridoxal has been developed. Acid phosphatase was used for dephosphorylation and precipitation of the serum proteins was not required. A chloramphenicol-resistant strain of L. casei was used as the test organism and this removed the need for sterilization. The method gives highly reproducible results, and is suitable for population and institutional studies.
An automated method for the microbiological assay of vitamin B(12) is described. A chloramphenicol-resistant strain of Lactobacillus leichmannii is used as the test organism and this eliminates the need for sterilization or aseptic addition. Precipitation of the serum protein is avoided by dilution of the serum with a glutamic/malic acid solution before heating the preparation to free the vitamin B(12). Tests can be set up at a rate of 80 an hour and after incubation they can be read at 160 an hour. By interfacing a programmed electronic calculator directly with the Mecolab M which is used for the assays, results are printed directly in ng/l.