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Biomedical subjects

R E Crandall

Publications and source records attributed to R E Crandall.

7 recordsLinked to original sources
PubMed1991

Fascicular topography of the median nerve: a computer based study to identify branching patterns.

This study was undertaken to definitively describe the internal anatomy of the median nerve. Thirty median nerves were dissected in the distal forearm and hand. Sixteen nerves were sectioned every 250 to 1000 microns along their length. Computer programs were developed in our laboratory to digitize and store the data. Analysis of our data has shown the following consistent findings: (1) the number of fascicles remains relatively constant over the centimeter lengths of the nerve; (2) over 75% of the sections contain zero to one fascicular branching; (3) fascicular groups destined for the same endpoint remain localized within the nerve for long distances; and (4) calculation of Wilks' lambda demonstrates a high degree of group fascicular regionality.

Humans↗
PubMed1988

Role of radionuclide imaging in the evaluation of wrist pain.

The cause of hand and wrist pain can be difficult to determine, especially when standard radiographs are normal or show only nonspecific changes. This study reports the effectiveness of radionuclide imaging in the evaluation of patients with hand and wrist pain of uncertain cause. Eighty-eight patients with hand and wrist pain and initially normal standard radiographs were evaluated prospectively by additional radiographic methods including the following: routine tomography, wrist arthrography, computerized tomography, or magnetic resonance imaging. Each patient also had bone scintigraphy. The diagnosis established by clinical assessment and by other imaging methods was then compared with the scintigraphic findings. The presence or absence of focal scintigraphic abnormalities correlated with the presence or absence of focal pathology definable by the conventional methods in 88% of patients. As expected, scintigraphy was chiefly of value in defining the locus of an injury or other process in the wrist, rather than the nature of an abnormality. The scintigrams were abnormal in 95% of cases involving complete intrinsic ligament ruptures and fractures and were normal in 96% of patients with no definable injury. Scintigraphic findings correlated poorly with partial intrinsic ligament injuries and in cases of synovitis. Radionuclide imaging is a sensitive means of detecting focal lesions in patients with hand and wrist pain of unknown cause.

Adult↗
PubMed1988

Multiple nerve dysfunction after carpal tunnel release.

A case is reported of the delayed onset of multiple nerve dysfunction after simple carpal tunnel release. The patient received a local anesthetic with the use of an arm tourniquet. The multiple dysfunctions included radial sensory and motor dysfunction at 5 days, anterior interosseous nerve syndrome at 33 days, and ulna intrinsic motor loss between 33 and 77 days after operation. There was no recovery in the radial or anterior interosseous nerves after 5 months of conservative management. At operation the radial nerve was compressed by the intermuscular septum, and the anterior interosseous nerve was compressed by the superficialis arcade. Decompression was followed by complete recovery.

Adult↗
PubMed1984

GELYSIS: Pascal-implemented analysis of one-dimensional electrophoresis gels.

GELYSIS (GEL analYSIS) is a Pascal implementation of an algorithm that takes data on the distances migrated by DNA fragments in a one-dimension electrophoretic gel and calculates the sizes of the fragments based on the known sizes of marker fragments. A key step in the overall algorithm is a cubic-spline best-fit of marker calibration data. An option of the program is the generation of graphic representations of the gel and of the calibration spline that are convenient for primary data storage or for publication. Provision is made for specifying different band intensities in the graphic output of the gel.

Base Sequence↗
PubMed1981

The effects of radioiodination and fluorescent labelling on albumin.

The preparation and characterization of fluorescamine -, fluorescein isothiocyanate (FITC) -, and radioiodine-labelled bovine serum albumin is critically evaluated. Electrophoretic mobility and ion-exchange chromatography, together with measures of degree of conjugation and sulfhydryl content, are used to assess the changes due to conjugation. Fluorescamine labelling results in drastic changes in chromatographic behavior and electrophoretic mobility. FITC labelling also results in significant changes in chromatographic and electrophoretic properties. Radioiodination leads to minor changes in chromatographic properties and oxydation of sulfhydryl groups, with little or no change in electrophoretic properties. All three labels have some degree of lability and show increased levels of free label with time, even after extensive initial purification. It is concluded that the two fluorescent labels and possibly the radioiodine labelling method used here are unsuitable for certain studies of BSA, such as its adsorption at solid-liquid interfaces.

Chemical Phenomena↗
PubMed1980

An analysis of the heterogeneity of albumin.

A simple and rapid procedure for characterization of serum albumin samples and for the isolation of fractions from fresh serum or commercial sources has been developed, based on DEAE Sepharose CL-6B ion exchange chromatography. Characterization of the fractions included sulfhydryl analysis, gel electrophoresis, and immunoelectrophoresis. Both analytical and preparative procedures were used. It has been shown that the relative content of the fractions varies from individual to individual. It has also been shown that certain fractions found in commercial preparations are artifacts which should be removed before performing serious studies with serum albumin.

Animals↗