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Biomedical subjects

R E Cooley

Publications and source records attributed to R E Cooley.

6 recordsLinked to original sources

Expertise in expert systems: knowledge acquisition for biological expert systems.

In this paper it is argued that an expert system requires more than factual knowledge before it can display expertise in a given domain. The additional knowledge consists of the heuristics or 'rules of thumb' used by an expert to manipulate and interpret the factual knowledge. The knowledge acquisition phase of an expert system project involves determining the factual knowledge (which may be obtained from published sources) and the heuristics used by an expert to manipulate that knowledge--these heuristics can only be obtained from an expert. In reviewing existing biological expert systems it is apparent that many contain only the factual knowledge relating to the domain, and lack the heuristics that enable such systems to show expertise. This paper reviews a number of knowledge acquisition techniques which could be used for acquiring heuristic knowledge and discusses when their use is appropriate. The knowledge acquisition techniques discussed are those suitable for the development of small-scale expert systems as these are most likely to be of interest to biologists. The techniques include the use of questionnaires, interview techniques and protocol analysis; particular emphasis is placed on a modification to the 'twenty questions' interview technique which was developed specifically to elicit taxonomic knowledge relating to water mite identification.

Biology↗

Measurements of multiply scattered light for on-line monitoring of changes in size distribution of cell debris suspension.

Dual wavelength frequency-domain measurements of photon migration (FDPM) are conducted on filtrate samples obtained from an industrial centrifugation process designed to separate Escherichia coli cell debris from the inclusion bodies. FDPM measurements consist of detecting phase delay of intensity-modulated light at 670 and 820 (or 830) nm. Optical properties of isotropic scattering and absorption are obtained from the regression of phase delay data to the optical diffusion equation. We show that the corresponding intensity-based measurements alone cannot provide accurate and independent estimates for these optical properties. However, FDPM-derived scattering coefficients of filtrate solutions (primarily consisting of 0.1-0.2 micrometer E. coli cell debris) are sensitive to approximately 1 vol % of added inclusion bodies (of 1-2 micrometer size). The technique, theory, and future adaptation of FDPM as an on-line monitor to detect the loss of inclusion bodies in centrifugation following homogenization are presented and contrasted to conventional, intensity-based measurements.

Biotechnology↗