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Biomedical subjects

R E Benesch

Publications and source records attributed to R E Benesch.

At least 55 records · Page 3Linked to original sources
PubMed1975

Interaction of hemoglobin with three ligans: organic phosphates and the Bohr effect.

The assumption that the Bohy coefficient (less than log p 50)/(less than pH) is equal to the haldane coefficient (less than h minus plus) of hemoglobin is shown to be incorrect in the presence of allosteric effectors such as 2,3-diphosphoglycerate. The theoretical relation between the two coefficients in the presence of 2.3-diphosphoglycerate as derived. Experimental data on the variation of both coefficients with diphosphoglycerate concentration are presented and shown to be in agreement with prediction.

Diphosphoglyceric Acids↗
PubMed1974

Chemical modifications that inhibit gelation of sickle hemoglobin.

Substitution of the N-terminal amino groups with pyridoxal compounds inhibits gelation and increases the solubility of deoxy sickle hemoglobin (Hb S). Pyridoxylation of the alpha chains has considerably more effect than that of the beta chains. The increase in minimum gelling concentration of Hb S that results from modification of the alpha N-termini is the same as that produced by dilution of Hb S with an equal amount of Hb A.

Amino Acid Sequence↗
PubMed1973

Pyridoxal compounds as specific reagents for the alpha and beta N-termini of hemoglobin.

Bifunctional pyridoxal derivatives with a charged side chain in the 5' position react specifically with the N-terminal valine residues of hemoglobin. Three of these compounds, which have only a single negative charge in the side chain, are highly selective for the alpha-chain N-terminal residues in the oxy conformation while pyridoxal phosphate, with two negative charges, reacts specifically with the beta N-terminal in deoxyhemoglobin. Schiff's base formation, therefore, results in decreased oxygen affinity with pyridoxal phosphate but increased oxygen affinity with the other three compounds. Reduction with sodium borohydride leads to irreversible coupling at either end of the molecule, and preparation and properties of such derivatives are described.

Binding Sites↗