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Biomedical subjects

R Durand

Publications and source records attributed to R Durand.

At least 91 records · Page 5Linked to original sources
PubMed1979

Phosphate transport and proteins with SH groups in rat liver mitochondria.

Phosphate transport in rat liver mitochondria was studied by following [32P] phosphate uptake within physiological concentrations. Transport inhibition due to mersalyl and protection by mersalyl against N-ethylmaleimide measured in those conditions corresponded to earlier results obtained by the swelling technique. When mitochondria were incubated with [3H] N-ethylmaleimide in the presence of mersalyl, the radioactive labeling in proteins of particles obtained after sonication was decreased in all fractions, but three proteins were both highly alkylated and also highly protected by mersalyl (M.W. 48,000 - 36,000 - 31,000). Two of these (M.W. 36,000 and 31,000) were partially purified by ultrogel chromatography in the presence of sodium dodecyl sulfate. Furthermore, it was shown that both phosphate and nigericin diminished labeling by N-ethylmaleimide in the final supernatant fraction. Two proteins (M.W. 98,000 and 31,000) were significantly alkylated by [3H] N-ethylmaleimide and protected by phosphate and nigericin.

Animals↗
PubMed1979

Comparative effects of ionophores grisorixin, alborixin and two derivatives on K+ glutamate efflux in rat liver mitochondria.

The alkali cations discrimination on a liquid membrane electrodes system, was determined for the carboxylic ionophores grisorixin, alborixin and two derivatives, dihydrogrisorixin and hexahydroalborixin. The two antibiotics exhibited a great perference for K+. Dihydrogrisorixin again showed the selectivity curve of a carboxylic ionophore, but with a discrimination power lowered compared with grisorixin. Hexahydroalborixin had lost all the complexing properties of the natural molecule. The selectivity scales measured for cations, were directly correlated with the K+ and glutamate effluxes measured in rat liver mitochondria. The chemical modifications of the natural structures of grisorixin and alborixin resulted in a drastic reduction of their ionophoric properties. The loss of K+-glutamate might occur in two steps, the efflux of K+ catalysed by the ionophores then causing a loss of negative charges in the form of glutamate.

Animals↗
PubMed1976

[Effects of light on reproductive processes in fungi; hypotheses on the identity of photoreceptors. A bibliographic revue].

The present review describes the effects of light on reproductive processes in fungi, mainly when action spectra are available. The study of these has resulted in three kinds of photoresponses observable in fungi: - responses only to UV light (230-380 nm) - responses to NUV and blue light (300-520nm) - responses to great wavelengths of the visible spectrum. The photomorphogenetic processes on the control of these same photoreceptor pigments are reviewed.

Fungi↗
PubMed1976

Proteins dealing with N-ethylmaleimide inhibition of pig heart mitochondrial phosphate transport system.

Our data clearly demonstrate that protective effect of phosphate and protective effect of mersalyl against NEM-inhibition of phosphate transport act at the level of two kinds of proteins. (1)Two major components are phosphate and nigericin NEM sensitive. According to our previous data [13] it has been also demonstrated that these two proteins components are valinomycin NEM sensitive (results not shown here) suggesting a relationship between these proteins and the energy linked proton translocation process. Relationships between these proteins and the phosphate translocation process are not evident and are under further investigations. (2) Two other insoluble major components localised at the level of the subparticular fraction are mersalyl NEM sensitive. We can suggest that these proteins are implicated in the translocation of phosphate in pig heart mitochondria.

Animals↗