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Biomedical subjects

R Dasgupta

Publications and source records attributed to R Dasgupta.

41 records · Page 3Linked to original sources
PubMed1976

Blocked 5' termini in brome mosaic virus RNA.

All four components of brome mosaic virus RNA have m(7)G(5') ppp (5')Gp as their 5' terminus. The m(7)G can be removed by beta-elimination, resulting in the conversion to pppGp.

Base Sequence↗
PubMed1976

7-Methyl-guanosine and efficiency of RNA translation.

Brome mosaic virus RNAs 3 and 4 were chemically modified to remove the terminal 7-methyl-guanosine (m7G) structure, and the modified RNAs were tested for their messenger activity in a cell-free system derived from wheat embryo. Amino acid incorporation and ribosome-binding data show that removal of m7G results in reduction, but not complete abolition, of the messenger activity of the RNA. This suggests that the function of m7G may be related to efficient translation of messenger RNA. Possible involvement of other structural factors in RNA translation is discussed.

Cell-Free System↗
PubMed1975

Nucleotide sequence of a viral RNA fragment that binds to eukaryotic ribosomes.

The nucleotide sequence has been determined for the first 53 bases of brome mosaic virus RNA4, the monocistronic messenger for brome mosaic virus coat protein. The sequence includes the binding site for wheat embryo ribosomes. The 5'-terminal base is a modified guanosine attached to the penultimate base through a 5' p-p-p 5' link. The initiating AUG codon is only 10 nucleotides from the 5'-terminus. The triplets following the AUG codon correspond to the known sequence of brome mosaic virus coat protein.

Amino Acid Sequence↗