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Biomedical subjects

R D Woittiez

Publications and source records attributed to R D Woittiez.

At least 19 recordsLinked to original sources
PubMed1989

Growth of medial gastrocnemius muscle and Achilles tendon in Wistar rats.

Dimensions, architecture, material properties and functional characteristics of medial gastrocnemius muscles and Achilles tendons of young and old Wistar rats were compared. Dimensions associated with length of the leg segment grow isometric with it. Muscle belly transsectional dimensions, volume of muscle tissue and associated functional indices increase out of proportion either with the dimensions of the limb segment or the weight of the animal. As the properties of the contractile tissue remain the same and relative proportions of tissue components alter, changes in architecture result. These are not reflected in transsection of the tendon. Functional characteristics of the muscle tendon complex can be explained by architectural or dimensional-peculiarities emerging in the complex by growth processes of which the main is that the muscle belly of this pennated muscle grows in length mainly by increments in girth of the muscle fibers proper.

Achilles Tendon↗
PubMed1988

Influence of muscle dimensions on economy of isometric exercise in rat medial gastrocnemius muscles in situ.

The effect of muscle dimensions on economy (force-time integral divided by the amount of energy utilized) was investigated in male rats (body mass range 95-490 g), anaesthetized with pentobarbital. The medial gastrocnemius muscle in situ performed 6 maximal isometric contractions of 350 ms duration (1.s-1) at twitch optimum length at 35 degrees C. The areas under the 6 time-force curves were added to obtain force-time integral of the experiment. Differences of concentrations of ATP, phosphocreatine and lactate between experimental and contralateral (resting) muscles were used to calculate high-energy phosphate consumption due to stimulation. Muscle mass and cross-sectional area increased (approximately +400% and +300%, respectively) over the rat body mass range studied. Muscle length and length of the most distal fibre bundle increased by approximately 17 mm and 4 mm, respectively. Force-time integral (N.s) increased proportional to cross-sectional area whereas high-energy phosphate consumption (mumoles) increased proportional to muscle mass. The relative fraction of the total energy consumption utilized for force-independent processes was independent of rat body mass. The economy of the actomyosin system was unaffected during growth, whereas economy of the whole muscle decreased during growth by approximately 30% (p less than 0.001). The effect of muscle dimensions on economy is discussed with respect to human endurance capacity measured by voluntary isometric contractions.

Animals↗
PubMed1987

Functional characteristics of rat gastrocnemius and tibialis anterior muscles during growth.

Several morphological and functional characteristics of the rat gastrocnemius medialis and tibialis anterior muscle were studied in young, adult, and old rats to assess the influence of growth. Antagonist muscles were studied to determine how changes of muscle architecture and functional characteristics are influenced by the demands of increased body weight and by the specific roles of these muscles in locomotion. Both muscles change drastically, for instance, in muscle length, volume, physiological cross-sectional area aponeurosis length, and their muscular geometry changes allometrically for both muscles. The relationships between muscle length, distance between origin and insertion, tendon length, and tibial length also change with growth. Both muscles are rather pennate, so that the increase of physiological cross-sectional area is a major factor in the determination of muscle length. No significant difference could be shown for fundamental physiological characteristics (i.e., functional characteristics normalized for muscular dimensions such as maximal work per unit volume). The changes of morphological and functional variables of both muscles parallel each other as is apparent from the index of antagonist characteristics, which is constant for all variables studied with the exception of muscle volume and tendon length. Consequently, the considerable and similar changes of TA and GM morphology and functional characteristics that take place during growth from approximately four weeks postnatally is not caused by changes of muscular material but by changes of the amount and architectural arrangement of the material involved.

Animals↗
PubMed1987

Mechanical properties of passive rat muscle during sinusoidal stretching.

The dynamic passive response of the left gastrocnemius medialis muscle of thirty male Wistar rats was studied as a function of muscle dimensions and absolute and relative amount of connective tissue. Values of the absolute active and passive length-force curves (active force, passive force, active working range) correlated well (coefficients of correlation in a range of 0.62-0.92) with morphological variables (such as muscle optimum length, mean muscle fibre optimum length, physiological cross section, muscle weight and amount of intramuscular connective tissue). To eliminate dimensional effects the active and passive length-force curves were normalized taking maximal active twitch force and muscle optimum length as reference values (100%). The width of the normalized active length-force curve (relative active working range) was correlated negatively with muscle weight, muscle optimum length and physiological cross section. Relative amount of connective tissue and passive tension at optimum length (both independent of muscle dimensions) were positively correlated, indicating that passive muscles are stiffer when relative amount of intramuscular connective tissue is higher. Sinusoidal movements with several amplitudes and frequencies of movement were imposed on the passive gastrocnemius medialis muscle over a range of muscle lengths. In accordance with the approximately exponential increase of static passive muscle force with length, muscle length has a large influence on the shape and magnitude of the hysteresis diagrams resulting from sinusoidal movements: the value of all variables selected increases approximately exponentially with muscle length with the exception of the value of loss tangent, a factor indicating the amount of energy dissipated during each cycle relative to the amount of energy stored and released elastically. Velocity of movement has only minor influence on variables of the hysteresis diagrams as is shown by changing the frequency of movement. As loss tangent and relative amount of connective tissue did not vary with muscle dimensions in the muscles studied, it is likely that material properties of the components causing passive resistance were similar in these muscles.

Animals↗
PubMed1987

A multipurpose muscle ergometer.

A fast (0.1 mm steps within 2 ms), strong (40 N continuously) and accurate (resolution 0.002 N and 1.0 micron) muscle ergometer was developed to test dynamic and static properties of mammalian muscle. Both for twitches and for tetani isometric, isokinetic and isotonic contractions can be measured accurately. Force-velocity data and time to peak-force data of four EDL muscles, as well as force-extension data of their serial tendinous structures are shown to demonstrate the machine.

Animals↗
PubMed1987

Passive resistance of the human knee: the effect of remobilization.

Changes of circumferential dimensions and passive resistance of the human knee caused by immobilization, were studied during remobilization. Patients immobilized with a long leg cast after tibial fractures or ligamentous injuries were studied immediately after removal of the cast and after mean periods of 18, 36 and 81 days of remobilization. Immobilization resulted in a decrease of circumferential dimensions. The difference in mid-thigh circumference between the immobilized and the unaffected leg was still present after 81 days of remobilization both for the patients with tibial fractures and for the remobilization both for the patients with tibial fractures and for the patients with ligamentous lesions. An increase of midpatellar circumference was present exclusively in the patients with ligamentous lesions at all four testing dates, indicating that this is an effect of the ligamentous lesion and not of immobilization per se. Variables of the hysteresis diagrams, resulting from sinusoidal movement of the knee at a range of knee angles, were used to quantify passive resistance of the knee in the flexion-extension plane (the muscles crossing the knee are inactive). Variables related to the elastic storage and release of energy, and variables related to energy dissipation were discerned. During remobilization the increased resistance to flexion (shown by the variables related to the elastic storage of energy), as found immediately after removal of the cast, disappears and the resistance becomes identical to the resistance of the unaffected leg. This may indicate a rapid readaptation of the length of ventral structures (shortened due to immobilization in a shortened position) to almost normal values.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1986

Functional morphology of the M. gastrocnemius medialis of the rat during growth.

Length-force relations, both active and passive, and twitch contraction characteristics were quantified for left medial gastrocnemius muscles of four young, four adult, and four old male Wistar rats. Muscle and bundle optimum length and muscle weight were also determined and subsequently used for calculation of a number of morphological characteristics of the muscles. Fiber optimum length was derived from muscle bundle optimum length. Generally, physiological characteristics remained constant during growth. There was no change either in active tension at muscle optimum length or in active working range relative to fiber optimum length, relative passive fiber stiffness, active force relative to passive force at optimum length, twitch contraction time and twitch half relaxation time at optimum length. A number of morphological changes, however, did take place in the medial gastrocnemius muscle during growth. Fiber optimum length increased but only by about 2 mm from youth to old age, whereas muscle optimum length increased by approximately 14 mm, presumably owing to extensive hypertrophy of the muscle fibers during growth. The priority for force of the medial gastrocnemius muscle (defined as the quotient of physiological cross-sectional area of a muscle and the cubed root of its volume, a measure independent of architecture and dimensions of muscles) increased during growth. This increase indicates that during growth the muscle shifts relatively more towards force generation than towards excursion generation. These findings are discussed in view of existing scaling theories.

Aging↗
PubMed1986

Motor endplate position of rat gastrocnemius muscle.

In this study, the relative endplate position of fibers of rat gastrocnemius caput mediale (GM) muscle was determined by counting numbers of sarcomeres. Isolated fibers were teased from the proximal, intermediate, and distal regions of the muscle. Endplates of distal fibers were located on the proximal third of their lengths. Endplates of intermediate fibers were located at half fiber length, and for proximal fibers, a variable endplate position was obtained: in half of the muscles studied, endplates occurred around the proximal one-third and in the other half near the midpoint of the fiber. Endplate position relative to fiber length was thus found to be dependent on the region of the muscle. Changes in the orientation of endplate zone relative to the muscle belly is likely to take place with changes in muscle length, as shown by a planimetric muscle model. It is argued that architecture of pennate muscles may highly affect characteristics of motor unit potentials.

Animals↗
PubMed1986

Muscle economy of isometric contractions as a function of stimulation time and relative muscle length.

For rat medial gastrocnemius muscle economy (i.e. the ratio of time integral of force and total energy-rich phosphate consumption) was calculated. Muscles in situ at 35 degrees C were stimulated to perform either one continuous or several repetitive isometric contractions at one muscle length in the range from 70% to 130% of optimum muscle length for force generation. Whereas during one continuous contraction economy increased, no differences in economy were found between 6, 12 or 18 successive contractions. Economy during intermittent exercise was always lower than during continuous exercise. The difference in economy is a result of different rates of metabolism, whereas no difference was found for force generation. Economy was highest at optimum muscle length for force generation and decreased at muscle lengths smaller as well as greater than optimum muscle length. Force-dependent energy consumption was calculated by subtracting the force-independent part (obtained by extrapolation) from total energy consumption. The calculated force produced per mumol force-dependent energy-rich phosphate consumption was similar in muscles stretched beyond optimum length. In contrast, a decreasing amount of force per mumol force-dependent energy-rich phosphate consumption was observed at lengths smaller than optimum length.

Animals↗
PubMed1986

Passive resistance of the human knee: the effect of immobilization.

The effects of immobilization on both the circumferential dimensions of the lower limb, and the passive resistance of the knee to sinusoidal motion in the flexion-extension plane, were studied in patients immobilized after tibial fractures or ligamentous injuries of the knee. Immobilization resulted in a decrease of circumferential dimensions of the lower limb, indicating atrophy of thigh and calf musculature. The equilibrium angle of the knee, i.e. the angle at which the net passive moment equals zero, approached the angle of the knee during immobilization. At knee angles of 60 degrees (the maximal angle imposed on the immobilized knee) and 45 degrees, the resistance was increased, and at angles of 30 degrees and 15 degrees, the resistance was decreased. The passive resistances at +/- 22.5, 15 and 7.5 degrees relative to the equilibrium angle were all increased. A simple model, taking into account changes in the length of muscles at the anterior and posterior side of the knee, explains changes in passive resistance. The duration of immobilization for patients with ligamentous injuries of the knee was less than 50% of that for patients with tibial fractures, whose ankle joint was not immobilized, both resulted in a smaller muscular atrophy for patients with ligamentous injuries. Differences in passive resistance between unaffected and immobilized legs were the same in both groups of patients. Changes in passive resistance in the immobilized leg for those with ligamentous injury are probably not the effect of immobilization alone but the combined effect of immobilization and ligamentous lesions.

Adolescent↗
PubMed1985

Functional characteristics of the calf muscles of the rat.

Length-force relations, both active and passive, and twitch contraction characteristics were quantified for the entire complex of the superficial calf muscles, as well as individually for the Mm. soleus, plantaris, and gastrocnemius, caput mediale and laterale, of eight male Wistar rats. The M. soleus composes approximately 5% of the weight and cross-sectional area of the entire group of superficial calf muscles and is the only muscle of the group containing mainly slow-twitch fibers. The other superficial muscles of the calf are primarily fast-twitch muscles. The mono-articular M. soleus, the bi-articular M. gastrocnemius, caput mediale and laterale, and the poly-articular M. plantaris differ with respect to the number of joints crossed. However, contrary to the findings for cat hind limbs (Goslow et al. [1977] J. Morphol. 153:23-38), the muscles of the complex of superficial calf muscles of the rat did not differ with respect to a) their fiber optimum length, b) their maximum length range of active force generation, c) the relative increase of passive force owing to lengthening of the muscle, d) the angle of the ankle at which they produce maximal active force (the knee angle was fixed at 90 degrees).

Animals↗
PubMed1985

The instantaneous torque-angular velocity relation in plantar flexion during jumping.

Torques, angular velocities, and power of the ankle joint during plantar flexion were measured in jumping experiments in order to achieve insight into shape and magnitude of the instantaneous torque-angular velocity relation in a complex movement. Twelve trained subjects performed maximal vertical jumps from a semi-squatting position with 100 degrees of flexion in the knee joint. Ground reaction force measurements and film analyses were used to calculate instantaneous torques, angular velocities, and power outputs during plantar flexion. The shape of the instantaneous torque-angular velocity was different from the well-known hyperbolic force-velocity relation for isolated muscles. Maximal power output (2499 +/- 751 [SD] W) occurred at 60% of the mean maximal torque (301 +/- 62 N X m) and 80% of the mean maximal angular velocity (970 degrees/s). The maximal power output was six times larger than the power output reported in the literature for maximal isokinetic (monoarticular) plantar flexions. Influences like storage of energy in the series elastic component of Hill's muscle model and the role of polyarticular muscles in transporting energy from knee to ankle are discussed. It is concluded that many more selective studies will be necessary before it is possible to relate intrinsic muscle properties to the performance of muscles in poly-articular complex movements.

Ankle Joint↗
PubMed1984

A three-dimensional muscle model: a quantified relation between form and function of skeletal muscles.

A three-dimensional muscle model with complex geometry is described and tested against experimental data. Using this model, several muscles were constructed. These muscles have equal optimum length but differ in architecture. The force exerted by the constructed muscles, in relation to their actual length and velocity of shortening, is discussed. Generally speaking, the constructed muscles with considerable pennation have great fiber angles, a great physiological cross section, a narrow active and steep passive length-force relation, and a low maximal velocity of shortening. The maximal power (force times velocity) delivered by the constructed muscles is shown to be almost independent of the architecture of the muscles. The steepness of the passive length-force relation is determined mainly by the shortest fibers within the group of constructed muscles, whereas maximal velocity of shortening and the width of the active length-force relation are determined mainly by the longest fibers. The validity of the three-dimensional muscle model with respect to some morphological and functional characteristics is tested. Length-force relations of constructed muscles are compared with the actual length-force relations of mm. gastrocnemii mediales and mm. semimembranosi of male Wistar rats. Moreover, actual fiber angle, fiber length, and muscle thickness of three mm. gastrocnemii mediales are compared with values found for constructed muscles. It is concluded that the three-dimensional muscle model closely approximates the actual muscle form and function.

Animals↗
PubMed1984

Twitch characteristics in relation to muscle architecture and actual muscle length.

The length dependence of twitch time characteristics is quantified for several skeletal muscles of the rat: lateral gastrocnemius, medial gastrocnemius, plantaris, soleus and semimembranosus. It is shown that muscle architecture influences the length dependent behaviour of twitch time characteristics of muscles. Twitch contraction time is less susceptible to length changes of the muscles than the twitch relaxation time. With the exception of the relaxation time in the second part of the relaxation, the twitch time characteristics behave different with respect to their dependence on fibre length, in fact muscles that differ in architecture. It is concluded that twitch time characteristics are dependent on the actual muscle length and therefore should be determined at a well defined length.

Animals↗
PubMed1984

The number of sarcomeres and architecture of the M. gastrocnemius of the rat.

The aim of this study was to investigate the number of sarcomeres of different regions (proximal, intermediate and distal third) of the M. gastrocnemius of the rat and compare them with in vivo measurements of the length of the most proximal and distal muscle bundles. These lengths were measured with the aid of dividers at the muscle resting length. The number of sarcomeres was calculated from the length of fibres (measured at 20 times enlargement) tested from HNO3-treated muscle and the average sarcomere length (determined from 80 microns samples taken along the fibres every 800 microns). Ten fibres were isolated from each of three regions of six muscles. All muscles showed the smallest number of sarcomeres in the proximal region of the muscle and increasingly higher numbers in the intermediate and distal parts. The number of sarcomeres in the proximal region is significantly (p less than 0.01) smaller than that of the distal region. These results agree with the results of in vivo length measurements of the most proximal and distal bundles (resp. 31 and 36% of the muscle resting length), the former being significantly (p less than .01) smaller. As there is no significant difference (p less than 0.01) in the length of the treated fibres of the three regions it is concluded that HNO3 treatment does affect the fibres of the muscle in the different regions in a non uniform fashion.

Animals↗