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Biomedical subjects

R D Miller

Publications and source records attributed to R D Miller.

At least 433 records · Page 24Linked to original sources
PubMedDate not supplied

Vecuronium: a new nondepolarizing neuromuscular-blocking agent. Clinical pharmacology, pharmacokinetics, cardiovascular effects and use in special clinical situations.

Vecuronium provides additional flexibility to the clinician using neuromuscular-blocking drugs. Its shorter duration of action, lack of significant cardiovascular effects and lack of dependence on the kidney for elimination provide clinical advantages over, or alternatives to, currently available, nondepolarizing neuromuscular-blocking drugs.

Adolescent↗
PubMedDate not supplied

Further characterization of T cell receptor chains of marsupials.

cDNA clones encoding T cell receptor alpha (TCRalpha) and beta (TCRbeta) from the South American opossum, Monodelphis domestica were isolated and characterized. A single clone isolated encoding a TCRalpha chain was full length, containing the complete V (variable), J (joining) and C (constant) regions. Three partial cDNA clones were isolated for TCRbeta which contained complete C sequences. Phylogenetic analysis of the TCR Valpha revealed that the M. domestica sequence and a sequence from the Australian brushtail possum, Trichosurus vulpecula, belong to separate Valpha families and intersperse with sequences from eutherian mammals. Similar to results described for marsupial and eutherian light chains, diversity at the V region of the TCR is ancient and maintained. In contrast phylogenetic analysis of the TCR Calpha and Cbeta sequences from M. domestica, T. vulpecula, and other vertebrates revealed that the marsupial TCR C grouped together forming a sister group to eutherian mammals.

Amino Acid Sequence↗
PubMedDate not supplied

Isolation and comparison of the IgM heavy chain constant regions from Australian (Trichosurus vulpecula) and American (Monodelphis domestica) marsupials.

cDNAs encoding IgM heavy chain constant region (Cmu) were isolated from two metatherians (marsupials)--the Australian common brushtail possum (Trichosurus vulpecula) and the South American grey short-tailed opossum (Monodelphis domestica). Analysis of the sequences suggested that they correspond to the secreted form of Cmu in both species. The domain size and structure of the marsupial Cmu sequences were compared with other Cmu sequences and a high degree of conservation throughout vertebrate evolution was observed. Amino acid sequence comparisons revealed a marked level of sequence similarity between the two marsupial sequences (79%), relatively high similarity between the marsupials and eutherians (63%), and lower similarities between marsupials and birds (45%), marsupials and amphibians (47%), marsupials and reptiles (45%) and marsupials and fish (37%). These data allow the incorporation of metatherians into the study of mammalian IgM evolution.

Amino Acid Sequence↗
PubMedDate not supplied

Major histocompatibility complex class I loci from the gray whale (Eschrichtius robustus).

Sequences from exons encoding the peptide binding region of MHC class I (MHC-I) molecules were isolated from California gray whale (Eschrichtius robustus) genomic DNA to initiate an investigation of variation in these genes in a cetacean. These represent the first mysticete MHC-I sequences to be reported. The analysis of gray whale MHC-I sequences suggests the presence of at least three loci, which share greatest similarity to MHC-I in the ungulates, consistent with current views on cetacean phylogenetics. The peptide binding region of MHC is the most polymorphic part of the molecule and analysis of the variation and synonymous to nonsynonymous substitution ratios in gray whale sequences found these genes to display polymorphism characteristics similar to that attributed to selection in other species.

Amino Acid Sequence↗