Search PubMed⌕ Search

Biomedical subjects

R D Meyers

Publications and source records attributed to R D Meyers.

9 recordsLinked to original sources
PubMed1981

Identification of the factor responsible for autogenous bleaching of glycollate oxidase.

Autogenous bleaching of glycollate oxidase (glycollate:oxygen oxidore-ductase, EC 1.1.3.1) is characterized by a loss of absorption due to enzyme-bound FMN and the presence of a lag in the catalytic assay. The extent of bleaching varies with different preparations. The following evidence indicates that bleaching is due to the presence of sulfite in the enzyme preparation which forms a reversible complex (EFMN + Y = EFMN . Y, Y = sulfite) with enzyme-bound FMN. Both EFMN . Y and authentic enzyme-sulfite complex exhibit similar spectral and catalytic properties. At pH 7.0 both complexes are decomposed by agents (2,6-dichlorophenolindophenol, H2O2) which cause sulfite oxidation. In the absence of such agents the complexes are extremely stable at pH 7.0 even during extensive dialysis. A pronounced decrease in complex stability is observed at pH 9.0 and 25 degrees C. Both sulfite and Y are readily removed by dialysis under these conditions. At lower temperatures both complexes exhibit an increase in stability at pH 9.0. Reversible association-dissociation is observed with EFMN . Y and EFMN . SO3 at pH 9.0, when the temperature is varied between 25 and 0 degrees C. The supernatant obtained after heat denaturation of EFMN . Y causes bleaching when mixed with EFMN, indicating that Y is present in the heat extract. A stable Bunte salt is formed by reacting sulfite with 4,4'-dithiodipyridine. Reaction of the latter with the heat extract yields a product with spectral and chromatographic properties identical to the authentic Bunte salt, indicating that the heat extract contains sulfite.

2,6-Dichloroindophenol↗
PubMed1980

Evaluation of 5,000 endodontic treatments: incidence of the opened tooth.

Five thousand endodontic cases treated by three endodontists were reviewed to determine the frequency of the need to leave teeth opened for drainage. The subsequent treatment of these teeth is described. Of the 5,000 cases reviewed, 518 were left open at some point during treatment. Two hundred and eighty-one cases (54.2 percent) remained closed after the first closure. Four hundred and twenty-six (82.2 percent) of the cases opened were eventually completed without surgical intervention. The need to prescribe antibiotics and perform a trephination is also stated.

Alveolar Process↗