A simple apparatus for providing blood diets at constant temperature and with different corticosterone levels to individual bed bug colonies (Hemiptera: Cimicidae).
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Biomedical subjects
Publications and source records attributed to R D Hall.
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Observations of 2 groups of dams and their litters were made every 3 hr around the clock on Days 1-20 postpartum. The dams fed either an 8% or a 25% casein diet for 5 weeks before mating and during gestation and lactation. Dams on the low protein diet spent more time in the nest actively nursing their young than did high protein dams, and they exhibited no deficits in other maternal behaviors. Five of 11 kinds of behavior developed more slowly in the undernourished pups than in the well-nourished ones, but the developmental delays were no longer than a few days. Circadian fluctuations were apparent in all of the pups' activities during the 3rd postnatal week as well as in grooming and horizontal movement, 2 behaviors that were present earlier, by 6-10 days of age.
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The Subhuman Primate Pregnancy Test was evaluated as a means of detecting urinary chorionic gonadotropin to aid in pregnancy diagnosis in owl monkeys. Using radioimmunoassay, the excretion pattern of chorionic gonadotropin from pregnant owl monekys was delineated, the hormone being detected from 16 weeks prepartum until birth. By comparison, the pregnancy test kit detected chorionic gonadotropin between the fourteenth week prepartum and the last week of gestation with 94% accuracy. In a 2-year study using a simplified urine collection technique, the Subhuman Primate Pregnancy Test was shown to be a valuable procedure for diagnosing pregnancy and detecting spontaneous abortions in owl monkeys.
Sera were electrophoretically separated and examined from 238 karyotyped Aotus trivirgatus and 29 unkaryotyped offspring. Albumin polymorphism was observed with high frequency and found to conform to a codominant allele mode of transmission. A unique alpha globulin was identified in Karyotypes I, VII and unkaryotyped offspring, of which one parent was a Karyotype I. This alpha globulin phenotype appears to be a dominant characteristic.
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Treatment of normal male Leghorn chickens with doses of estradiol ranging from .1 to 1.0 mg per week caused only a slight increase in resistance to infestation with northern fowl mites. The resistance phenomenon did not increase linearly with estradiol dose. Pullets were initially resistant to mite infestation; however, susceptibility was noted to increase markedly near the time of initial egg-production. These data indicate that sex hormones may be related to mite resistance in chickens, but that estrogen alone is probably not responsible for the difference in mite susceptibility between male and female birds.
Roosters from a line artificially selected for high initial antibody response to sheep red blood cells were more resistant to development of populations of Ornitbonyssus sylviarum (Canestrini and Fanzago) than were birds selected for low antibody response. Oral administration of corticosterone to test chickens at doses ranging from 10 to 40 ppm did not affect mite development. The steroid regimen was shown to reduce lymphocyte and testes mass as well as total weight gain. Postmortem measurements were not different for the 2 inbred lines. Analysis of data indicated that antibody competency alone probably was not responsbile for the observed differences in mite populations.
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Complement-fixing and complement-fixing inhibiting (CFI) antibodies were demonstrated in the clinical and convalescent stages, respectively, of rhesus monkeys infected with either monkey poxvirus, Tanapoxvirus, or Yaba poxvirus. Specificity of the CFI antibody was confirmed by its failure to cross-react with heterologous poxvirus antigens and by experiments demonstrating the CFI test as being antigen dependent. Serum containing CFI antibody neutralized homologous poxvirus but failed to agglutinate antigen-coated, tanned red blood cells. The application of CFI test as a seroepidemiologic tool for studies of poxvirus infection of man and simian monkeys and the biologic role of CFI antibody in pathogenesis were discussed.
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