Haemorrhagic pulmonary oedema ("massive pulmonary haemorrhage") in the newborn.
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Blood Proteins↗
Biomedical subjects
R D Boyd
Publications and source records attributed to R D Boyd.
Composition of alveolar liquid in the foetal lamb.
1. Experiments were performed on foetal lambs at gestations between 125 days and term. The foetus was exteriorized at Caesarean section with the umbilical cord and placental attachment maintained intact. Samples of liquid from the alveolar parts of the lung were withdrawn through a tracheal cannula and samples of lung lymph, plasma and amniotic liquid were also obtained. Measurements were made of total osmolality, concentrations of electrolytes and urea, pH and P(CO2). Titrations were carried out with N/10 HCl and N/10 NaOH. The water content of the liquids was estimated and concentrations expressed per kg H(2)O.2. In alveolar liquid [H(+)], [K(+)] and [Cl(-)] were higher and [Ca(2+)], [phosphates] and [HCO(3) (-)] were lower than in plasma or lymph. In amniotic liquid osmolality [Na(+)], [Cl(-)] and [Ca(2+)] were lower and [phosphates] higher than in plasma or lymph. Alveolar liquid/plasma ratios of [HCO(3) (-)], [Ca(2+)], [Cl(-)] and [K(+)] differed from ultra filtrate/plasma ratios of these ions.3. Titration curves demonstrated a very small amount of buffering in alveolar liquid at its in vivo pH of 6.27 mostly due to HCO(3) (-) at an average concentration of 2.8 mM/kg H(2)O.4. It is concluded that foetal alveolar liquid is not an ultrafiltrate of plasma nor a mixture of amniotic liquid and plasma ultrafiltrate, but a special material elaborated by the foetal lung.
Amniotic Fluid↗
Fetal and maternal blood volumes in shed human placentae: discrepant results comparing morphometry to haemoglobin content.
The maternal blood volume (MBV) and fetal blood volume (FBV) of shed human placentae delivered by caesarean section at term were measured using a morphometric technique and from the placental content of adult and fetal haemoglobin. MBV was 35.3 +/- 1.5 (s.e.m.) per cent by the former and 11.0 +/- 1.5 per cent by the latter technique. FBV was 11.0 +/- 0.7 and 9.0 +/- 0.6 per cent respectively (n = 6). Measurement of the dimensions of individual villi initially photomicrographed in 0.9 per cent NaCl and subsequently re-photographed in fixative suggested that individual villi shrank to 0.7 of their initial volume during fixation. It is suggested that morphometric measurement of MBV may lead to approximately a threefold overestimate because of relative MBV expansion and villous tissue shrinkage during the process of fixation without alteration in overall placental volume.
Blood Volume↗
Effect of maternal hypokalaemia on unidirectional maternofetal and net potassium fluxes across the placenta of the anaesthetized rat.
Potassium (K+) fluxes across the placenta of rats, at 21 days gestation, fed a low K+ diet or a control diet were studied. The rats on the low K+ diet had a significantly (P less than 0.001) lower arterial plasma K+ concentration compared to those on the control diet (1.95 +/- 0.12 and 2.93 +/- 0.06 mmol/l respectively; mean +/- s.e., n = 17). Fetal umbilical arterial plasma K+ concentration was unaltered in maternal hypokalaemia and was significantly (P less than 0.001) higher than that of maternal plasma (4.58 +/- 0.15 and 4.66 +/- 0.12 mmol/l in low K+ and control groups respectively). Net K+ flux across the placentas (as measured by fetal accretion between days 20 and 21 of gestation) of hypokalaemic mothers (0.106 +/- 0.02 mumol/min/g placenta, n = 6) was not different to that in controls (0.104 +/- 0.01 mumol/min/g placenta, n = 8). Unidirectional maternofetal flux (Jmf) across the placentas, measured as the accumulation of 42K in the fetuses after injection of the radioisotope into maternal blood, was also not significantly different between the hypokalaemic and control mothers (0.5 +/- 0.08 mumol/min/g placenta, n = 8 versus 0.63 +/- 0.06 mumol/min/g placenta, n = 7, respectively). However, measurement of Jmf by perfusion of the placentas through their fetal circulations yielded a higher value than the accumulation method and in this analysis was significantly (P less than 0.02) lower in the low K+ than in the control group (0.75 +/- 0.10 mumol/min/g placenta, n = 11, and 1.27 +/- 0.14 mumol/min/g placenta, n = 9, respectively). These results show that net placental K+ fluxes are unaltered during maternal hypokalaemia but suggest that unidirectional maternofetal fluxes may be reduced.
Animals↗
Chloride channels of high conductance in the microvillous membrane of term human placenta.
The patch clamp technique has been used to study ion channels in the undisturbed microvillous membrane of the human placental syncytiotrophoblast. In villi from 55 placentae delivered by caesarean section, high resistance seals were achieved in approximately 30 percent of attempts. Of these, a large conductance chloride channel was identified in seven inside-out and two 'cell' attached patches. The channel had the following properties: (a) a slope conductance of 313 +/- 9 pS, (b) the presence of sub-conductance states, (c) voltage dependency, being open predominantly between +/- 20 mV and inactivating at more extreme potentials and (d) inhibition by DIDS (4-acetamido-4'-diisothiocyanostilbene 2,2-disulphonic acid). These are characteristic features of 'maxi' chloride channels which have been identified in a variety of cell types (Gogelein, 1988). The role of the chloride channel in ion transport by or homeostasis of the syncytiotrophoblast has yet to be determined.
Chloride Channels↗
[Synovial membrane and cartilage changes in an arthrosis model. Instability and impact stress model].
UNLABELLED: Surgical instability and repetitive impulsive loading was used to generate degenerative changes in knee joints of rabbits. Light- and electronmicroscopy was carried out at synovial membrane and cartilage samples. The instabilised joints had early synovial inflammation, preceding overall cartilage changes. Synoviocytes were activated and showed more and dilatated cytoplasmatic organells. Invaded round cells--potentially cartilage degenerative--were seen. In the loading model no initial synovial inflammation could be seen. Focal cartilage changes were located at the weight bearing zone. Two different inductive pathways may be involved in cartilage breakdown comparing the two arthrosis models. Mechanical factors seemed to trigger cartilage changes in the loading model, an inflammatory synovial pathway is likely to initiate cartilage breakdown in the instability model. CLINICAL RELEVANCE: The synovial membrane has the key-position for the cartilage degeneration in posttraumatic joint affections. The therapeutic use of antiphlogistic substances is indicated to supreme inflammatory mediators from the synovial membrane. This therapeutic concept seemed not to be useful in primary mechanical induced osteoarthrosis.
Animals↗
Education and training in a white water world.
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Education, Medical↗