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Biomedical subjects

R D Bagnall

Publications and source records attributed to R D Bagnall.

23 records · Page 2Linked to original sources
PubMed1977

An approach to the soft tissue/synthetic material interface.

It is argued that chemical modification of soft tissue implants in the hope of obtaining an associated tissue response is unlikely to succeed as a method for studying the fundamentals of implant/tissue interactions. An alternative approach is proposed which places greater emphasis on the interfacial interactions (such as protein adsorption) which occur after implantation, in a manner paralleling current advances in knowledge of the blood/material interface. From simple arguments, it is proposed that the observed similarities in soft tissue response of hydrophobic materials may result from irreversible protein adsorption, and that if unusual tissue responses are possible they are likely to be found only with hydrophilic implants. The possiblity of a critical hydrophilic/hydrophobic character which an implant must possess for essentially irreversible protein adsorption is also discussessed.

Animals↗
PubMed1977

Adsorption of plasma proteins on hydrophobic surfaces. I. Albumin and gamma-globulin.

The adsorption of albumin and gamma-globulin from solution at the liquid/air and liquid/liquid interfaces has been studied by the pendant drop technique as a model for the solid-liquid system. It is concluded that each protein adsorbs in a constant manner at the air, isooctane, and methylene iodide interfaces at equilibrium (1 hr), and the surface tension components of the protein layer presented to the hydrophobic phase have been found. The range of hydrophobic surfaces for which such constant behavior might be expected has been deduced, and it is shown that most common polymers lie within this range. A new model for protein adsorption is proposed, and the implications for biomaterials are discussed.

Adsorption↗
PubMed1977

Double contrast preparations: an in vitro study of some antifoaming agents.

An apparatus is described for the study of foam formation in double contrast preparations in vitro. Antifoaming agents are shown to be of considerable benefit to the double contrast technique, silicone and silicone-free antifoamers being equally effective. Silicone antifoamers are preferred because of their wide clinical acceptability. Antifoamer formulation and concentration are found to be critical factors in determining efficacy of foam suppression, and may lead to erroneous results in the clinic.

Antifoaming Agents↗
PubMedDate not supplied

Mutation analysis and genetic service: the construction and use of national confidential databases of mutations and pedigrees.

The development of rapid mutation screening procedures allows the detection of mutations in large populations. This is particularly useful for inherited diseases of high mutational heterogeneity, such as haemophilia A and B, because the analysis of the very many different natural mutants clearly defines the features that are important to the function of the relevant gene and gene product. Furthermore, the characterization of the mutation in an index person from each affected family may lead to the construction of confidential databases of mutations and pedigrees that allow optimization of genetic service. We report how, motivated by the aforementioned concepts, we have planned and introduced in the UK a national strategy to optimize genetic service in both haemophilias and, in particular, we describe the principles that have guided us.

DNA Mutational Analysis↗