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Biomedical subjects

R Coelho

Publications and source records attributed to R Coelho.

15 recordsLinked to original sources
PubMed1994

Bone marrow transplantation with major ABO incompatibility. Experience of the Bone Marrow Transplantation Unit--Francisco Gentil Portuguese Institute of Oncology, Lisbon Centre.

Thirteen patients submitted to bone marrow transplantation (BMT), HLA A and B identical, with mixed negative lymphocyte culture and major ABO incompatibility were retrospectively evaluated. Gravity sedimentation by hydroxyethyl starch was used in almost all cases to deplete erythrocytes from bone marrow (12/13): a removal rate of 90%--corresponding to an erythrocyte residual volume of 27,4 rate ml--and a nucleated cell recovery of 76% (mean values) were obtained. All patients underwent a hyperhydration regimen and received corticosteroid prophylaxis before bone marrow infusion. Considering the most important immunohematological problems associated to this type of transplant, we verified that seven patients developed minor complications and among them only one had long term consequences. In comparison with the control group, ABO identical and with minor incompatibility, the study group had delayed onset of erythropoiesis and needed greater erythrocyte transfusion support. We concluded that major ABO incompatibility does not constitute a drawback to BMT success in most patients.

ABO Blood-Group System↗
PubMed1994

[Compliance to therapy -- its importance in research and clinical practice].

Compliance, defined as patients behaviour to treatment schedule over time, specially in chronic diseases, is an important parameter to be measured in clinical trials. Compliance to a specific drug may compromise the final efficacy expected result assumed by phase I II and III studies. The medication events monitors systems (MEMS) appeared to get a more specific and sensitive measurement than the classic counting pills and drug or markers levels to analyses of compliance. The authors made a revision of literature in order to evaluate the benefits of compliance measurement in trials (specially hypertension as a paradigm of chronic disease) and clinical practice. Partial compliance with antihypertensive treatment could be an obstacle to an effective control or to demonstrate long term efficacy on decreasing morbidity and mortality. A short discussion is made about the possible benefits of using MEMS on clinical trials to evaluate patient's quality of life and behaviour to long term treatment for chronic diseases.

Clinical Medicine↗
PubMed1993

A comparative study of four different staining methods for estimation of live yeast form cells of Paracoccidioides brasiliensis.

A comparative study of four different staining methods for estimation of live yeast form cells of Paracoccidioides brasiliensis was carried out. The staining methods used were fluorescent staining, vital dye exclusion tests with erythrosin B and by Janus green and lactophenol cotton blue staining. Colony forming units (cfu) of the yeast form of eight P. brasiliensis isolates on brain heart infusion agar (BHIA) supplemented with 4% horse serum plus 5% P. brasiliensis cell extract (BHIA + HS + EXT) were examined for reliability of staining in determining the number of live fungal units in eight different isolates. Cfu on BHIA + HS + EXT plates showed an excellent plating efficiency over 96% in all isolates tested. The percentage of the live cells indicated by fluorescent staining (FL) or vital dye exclusion test with erythrosin B (EB) or Janus green (JG-1) was lower than that of cfu. By contrast, the percentage due to modified dye exclusion test with Janus green (JG-2) and that due to lactophenol cotton blue staining (LPCB) showed a close correration to that of cfu. Our results indicate that the modified dye exclusion test with Janus green and lactophenol cotton blue staining are useful for estimating cell viability of yeast form cells of P. brasiliensis.

Azo Compounds↗
PubMed1989

Essential hypertension: the relationship of psychological factors to the severity of hypertension.

165 hypertensive patients attending one general practice in Portugal were found to report significantly higher scores on measures of neuroticism, anxiety, depression and general psychological distress than 152 normotensive patients at the same practice. Hypertensive patients with evidence of organ damage exhibited significantly higher depression scores than those without such damage. These differences between normotensives and hypertensives, and between hypertensive with and without organ damage are discussed and previous research in this area is reviewed.

Adult↗
PubMed1989

Detection of rejection after heart transplantation. A computerized precordial mapping experience.

A low-cost modular compact personal computer-based system for surface precordial mapping (SPM) developed by the authors was used to detect rejection periods, documented by endomyocardial biopsy (EB), in heart transplant patients on cyclosporine A therapy. Data were collected with a matrix of 35 Ag-AgCl electrodes positioned over the precordio with anatomical references within 12 hours of EB. For each acquisition the authors computed the root mean square waveform from all signals collected to assess the accuracy of the maximum value of the RMS waveform (mRMS) parameter to detect rejection episodes. A mRMS variation of 20% in consecutive mapping acquisitions was the decision criterion. In EB, only presence or absence of acute rejection was considered. Eleven heart transplant patients were studied and 46 acquisitions were done. The mRMS obtained with SPM showed a sensitivity of 69% and a specificity of 91%, with a positive predictive value of 75% and negative value of 88%. The results indicate that surface precordial mapping may be promising for monitoring rejection.

Adolescent↗
PubMed1982

[In vitro study of tubulin polymerisation. Effects of ATP, vinblastine and isaxonine (author's transl)].

The dual action of vinblastine on tubulin, namely disruption and aggregation, is analyzed by turbidimetry. The existence of two different binding sites for the alkaloid is confirmed. The high affinity site binds vinblastine at low concentrations, at which no effect is observed on depolymerized tubulin, but where pre-formed microtubules are disrupted. The low affinity site is associated with tubulin aggregation. The binding constants of these two sites have been evaluated. Isaxonine (N-isopropyl-amino-2 pyrimidine) partially inhibits the effects of vinblastine, both on microtubule disruption and tubulin aggregation.

Adenosine Triphosphate↗
PubMed1977

[Acceleration of the speed of neuronal growth by a new neurotropic substance: N-isopropyl-amino-2-pyrimidine].

16 hrs. after per os administration of 14C N-isopropyl-amino-2-pyrimidine (IAP) in the Rat, radioactivity of the sciatic nerve is significantly higher than in plasma and other organs. In vitro IAP (base, orthophosphate or dichloracetate) accelerates neurite outgrowth of explained spinal ganglion after 48 hrs. of incubation in nutritive medium. Results suggest that IAP incorporated in nerve cell acts on cellular mechanisms controlling nerve growth.

Animals↗
PubMedDate not supplied

[Preliminary report concerning the histologica patterns of an anthracotic pneumoconiosis observed in the area of Londrina, Brasil (author's transl)].

The A. presents a preliminary report concerning on the histologic patterns of an anthracotic pneumoconiosis found in necropsies of the "Hospital Universitário da Universidade de Londrina", Paraná, Brazil, and in some cases from the Legal Institute of the same town. The lesions, on its histologic pattern are comparable to those observed in the lung of coal and iron mines workers as described in the consulted literature. The cases studied (plantations workers and dwellers at rural and suburban areas) are proceeding from an essencially agricultural region, without detectable polluition by industry or others known factors. This agricultural zone presents some geophysic peculiarities and dust from the errosive soil, is a constant factor in the local athmosphere. The soil is so called "Terra Roxa" (red soil) and in its physicochemical composition there is a great amount of iron oxides, silica (silt, agril laceous material), aluminium, manganese, organic compounds. In this preliminary report the A. suggests further research for a better knowledge of the composition of the respirable air and if dust exposures are or not responsible for the lung lesions.

Autopsy↗
PubMedDate not supplied

[Essential thrombocythemia. Therapy with interferon alfa].

A clinical case of essential thrombocythaemia is presented to demonstrate good therapeutic response to subcutaneous Alpha-2b Interferon in a dosage of 3 x 10(6) units, three times a week. Based on this case and on a literature research we propose that Alpha-2b Interferon is a legitimate alternative to alkylating agents or radioactive phosphorus (32P), showing identical efficacy and less Leukemogen effect.

Female↗