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Biomedical subjects

R Cisterna

Publications and source records attributed to R Cisterna.

At least 145 records · Page 8Linked to original sources
PubMed1989

Isolation of dysgonic strains of Microsporum canis in Bilbao (Spain).

In this report, we describe five cases of dermatophytosis caused by dysgonic strains of Microsporum canis which occurred in the area of Bilbao (Spain). The clinical presentations and responses to treatment of these infections were apparently similar to those caused by typical strains of this species but the increasing prevalence of dysgonic and other atypical strains of M. canis and the relative stability of the dysgonic morphology are the important features shown in our study. Contact with animals was confirmed by all of the patients, but in only one case was this possible infectious origin demonstrated mycologically. One of the patients, a heroin abuser with AIDS, showed a simultaneous infection with Trichophyton mentagrophytes var. mentagrophytes.

Adult↗
PubMed1989

Effect of erythromycin treatment on specific immunologic response in mice.

Six week old Swiss mice were sensitized by subcutaneous injection of 10(7) sheep red blood cells without adjuvant. One hour after sensitization, the mice were treated with erythromycin lactobionate for ten days. The minimal (15 mg/kg/day) and maximal (57 mg/kg/day) doses clinically used were assayed. The daily dose of erythromycin was administered intraperitoneally, in two injections, one every 12 hours. The kinetics of delayed type hypersensitivity reaction, measured by means of the foot-pad test, was evaluated by challenging different groups of fourteen mice with an eliciting dose of 10(8) SRBC injected into the foot-pad on days 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 after sensitization. Total and 2-mercaptoethanol resistant haemagglutinating antibody titres were determined in sera obtained from mice immediately after measuring the delayed type hypersensitivity reaction. Treatment with maximal erythromycin dose gave rise to a significant enhancement of the cellular immune response, and also to an acceleration of the humoral antibody response. On the other hand, treatment with minimal erythromycin dose gave rise to a slight depression of the immune cellular response and also to a depression of the antibody production at the beginning of the humoral response.

Animals↗
PubMed1987

Detection of antibodies to Candida albicans germ tube in the diagnosis of systemic candidiasis.

Sera from 109 subjects were tested for the presence of anti-Candida albicans antibodies by an indirect immunofluorescence assay. Aliquots of the sera were adsorbed with heat-killed blastospores to remove the antibodies against the surface of the yeast-phase cell wall and tested for anti-germ tube cell wall antibodies. Unadsorbed sera stained the entire cell wall of yeast and germ-tubes. Immunoglobulin G (IgG) antibodies were found in all patients with systemic candidiasis and in 81.2% of patients with Candida albicans isolated from skin and mucous membranes. IgA and IgG were found in 67.4 and 57.1%, respectively, of controls without evidence of candidiasis. After the adsorption only sera from patients with systemic candidiasis showed antibodies, predominantly IgA, against germ tube cell wall. Adsorption of the sera thus increased the specificity, efficiency, and positive and negative predictive values of the test. The test achieved the highest sensitivity in adsorbed sera for the combination of IgA and IgG.

Adult↗
PubMed1986

Immune responses to yeast and mycelial forms of Candida albicans in intraperitoneally infected mice.

Candida albicans E-139 produced pure mycelial and yeast cultures in a low sulphate medium at different temperatures. The influence of the morphological phase, dose and viability of the fungi on the kinetic of delayed-type hypersensitivity (DTH) and anti-mycelial and anti-yeast antibodies have been studied in mice injected intraperitoneally. The mycelial form elicited higher DTH levels than the yeast phase. This effect seems to be related to its antigenic properties. The effect of dose on the immune response depends on the viability of the fungus. The mycelial cytoplasmic antigens were more effective than the yeast ones in detecting antibodies induced during the experiments, particularly during the later stages of the observation periods, suggesting that such antigens may be useful in the serodiagnosis of Candida infections.

Animals↗
PubMed1986

Effects of fasting and immobilization stresses on the phagocytic capacity of rat polymorphonuclear phagocyte monolayers.

The effect of two different types of acute stress (immobilization and fasting) on the polymorphonuclear leukocyte phagocytic function has been studied in male and female rats. With this aim, a subgroup of rats was under immobilization and fasting, another under complete energy deprivation and a third one (controls), exposed to the normal activity of the animal room, for 15 hours. The stress induction was assessed by controlling weight variations and gastric ulcers generation. Both stressors induced weight loss but only immobilization resulted in the development of gastric ulcer in all the animals studied. Phagocytosis was increased in male rats stressed by fasting and in immobilized female rats. In the remaining subgroups polymorphonuclear leukocyte cells showed a phagocytic capacity within the range of control values. Only comparison of the males group stressed by fasting with the male group stressed by fasting and immobilization showed a significant depression in phagocytosis.

Animals↗
PubMed1986

[Acquired immunodeficiency syndrome in childhood. Report of 3 cases and review of the literature].

Three infants with the acquired immunodeficiency syndrome (AIDS) are reported. Two cases belong to infants died with AIDS; one of them have as infection source multiple transfusions of blood during the neonatal period and the other case was an haemophilic children transfused several times with commercial factor VIII. The third case belong to an infant, four months old, with AIDS-related complex, born of mother intravenous drug abuser. The authors comments some characteristics about pediatric AIDS and the present problems.

Acquired Immunodeficiency Syndrome↗
PubMed1985

Cellular and humoral immune responses to Candida albicans in subcutaneously infected mice.

Pure mycelial and yeast cultures of Candida albicans were produced in a low sulphate medium. Groups of mice were injected subcutaneously with increasing doses of viable or heat-killed mycelial or yeast cells and the kinetics of delayed-type hypersensitivity (DTH), anti-mycelial and anti-yeast antibodies were studied. Both the dose and the morphological phase of C. albicans showed an influence on the development of the DTH, but the viability is the factor which showed the highest influence on this reaction, since on the one hand mice infected with viable yeast or mycelial cells developed higher DTH levels than mice injected with heat killed cells, and on the other hand this factor seems to play an important role in the kinetics of DTH response. The enzyme-linked immunosorbent assay has been adapted to detect antibodies to yeast and mycelial phase cytoplasmic antigens of C. albicans. In contrast with the DTH reactions, neither dose, morphological phase nor viability played an important role on the antibody titer developed. However, the use of mycelial cytoplasmic antigens seems to be better than the yeasts to detect anti-Candida antibodies over the last days studied.

Animals↗
PubMed1985

[Yersinia enterocolitica septicemia in a thalassemic girl].

We report a four-year-old girl, previously splenectomized because of thalassemia major, who was admitted with gastroenteritis, abdominal pain and high grade fever. At laparotomy she was found to have appendicitis and mesenteric adenitis. Blood and stool cultures grew yersinia enterocolitica. Clinical course was favourable under Ampicillin-Gentamycin treatment. The importance of iron metabolism in the pathogenesis of yersinia sepsis is stressed, being this topic reviewed.

Appendicitis↗
PubMed1984

[Exanthematous viral diseases and measles: serology].

A study of antibodies against measles virus and other viral agents is made to investigate possible existence of a false measles or "measles-like" in 48 children with this disease during an epidemic. Hemagglutination-inhibiting technique was used to determine rubella antibodies, and complement fixation technique to measure those produced against other diseases studied. Results were grouped according to the seroconversions found. The following serologic diagnoses were found: twenty eight cases of measles, one of rubella, four of Coxsackie-Echo, three of Epstein-Barr infection, two of Parainfluenza, and one of respiratory syncytial virus infection. Some of the patients with an infection due to Coxsackie-Echo or Epstein-Barr viruses were simultaneously affected by measles. Results are commented and discussed, concluding that there in not any fixed pattern in the development of viral antibodies response in the child. Four of the cases studied were "measles-like".

Antibodies, Viral↗
PubMed1983

Comparison of three tests for measuring footpad swelling in the mouse.

Three tests, footpad thickness, footpad weight and footpad dye accumulation have been compared for measuring the local inflammatory reaction developed in the hind footpads of the mouse after injection of increasing doses of heat killed Candida albicans yeasts. The 3 tests showed high correlation indicating that any one of them may be used to evaluate such inflammatory reactions.

Animals↗
PubMed1983

[Acute Campylobacter jejuni gastroenteritis in children. Study of 23 cases].

Twenty-three cases of acute Campylobacter jejuni (CJ) enteritis were studied over à 8 month-period in hospitalized children. 906 fecal cultures were collected during the same period from children aged 1 month to 14 years. CJ was isolated in 33 (3.6%). The authors analyse the epidemiologic, pathogenic, clinical and therapeutic aspects of the disease in the 23 hospitalized children. After Salmonella, CJ is one of the most frequent etiologic agents for bacterial acute enteritis; its pathogenic mechanism is that of an invasion, mainly enteric. The clinical picture consists of diarrhea, fever and abdominal pain, difficult to assess accurately in young children. The disease is usually limited to the digestive tract and recovery occurs without antibiotic therapy.

Acute Disease↗
PubMedDate not supplied

Candidal infection of bone. Assessment of serologic tests in diagnosis and management.

In this case report, 30 sera from a 25-year-old heroin abuser with intervertebral candidosis were treated for the presence of anti-Candida albicans antibodies by agglutination, counterimmunoelectrophoresis, and indirect immunofluorescent assay. Sera were also adsorbed with heat-killed blastospores to remove antibodies against yeast-phase cells and tested by indirect immunofluorescent assay for anti-C. albicans germ tube antibodies (CAGTAs). Humoral responses to candidal 47-kD antigen were studied by immunoblotting in 23 unadsorbed sera. Anti-C. albicans antibodies were found in high titers by the three procedures but correlated poorly with the clinical evolution of the disease. CAGTAs were present from the beginning of the infection: Titers decreased in association with antifungal treatment and the patient's improvement, eventually becoming negative. Only class IgG antibodies to the 47-kD antigen were detected. These were present during the full course of the infection, failing to disappear at the end of the study. In this case, detection of CAGTAs appeared to be an important aid to diagnosis of the bony candidal infection, as they are detected early during the illness and seemed to have a prognostic significance.

Adult↗
PubMedDate not supplied

Seroprevalence of hepatitis B and C, and human immunodeficiency type 1 viruses in a rural population from the Republic of Equatorial Guinea.

The seroprevalence of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus type 1 (HIV-1) markers was evaluated in a group of 2042 subjects from a rural area in the Republic of Equatorial Guinea, to obtain a better understanding of the transmission patterns of these viruses. Antibodies to HIV-1 were detected in 12 subjects (0.6%); the seroprevalence did not differ significantly by age or gender. Overall seroprevalence for HCV was 1.7% (in patients aged > 40 years, 5.6%). Hepatitis B surface antigen was detected in 8.8% of subjects, with the higher seroprevalence in children aged < or = 18 years of 13.4% contrasting with the higher seroprevalence of HCV in older subjects of the Equatoguinean studied population. These results indicate differences in the distribution of the viruses and, probably, different routes of transmission. The study demonstrates the existence of a high HBV carrier rate in children, concluding that hepatitis B vaccine should be incorporated into the Expanded Programme on Vaccination in Equatorial Guinea.

Adolescent↗