Biomedical subjects

R Chetty

Publications and source records attributed to R Chetty.

184 records · Page 11Linked to original sources

PubMedDate not supplied

Clear cell (glycogen-rich) rhabdomyosarcoma presenting as cervical lymphadenopathy.

A 15-year-old patient is described who presented with cervical lymphadenopathy. A lymph node biopsy confirmed a metastatic neoplasm composed of islands and aggregates of round to polygonal-shaped cells with surrounding hyalinised fibrous stroma. The tumour cells contained a clear glycogen-rich cytoplasm and stained for desmin, muscle-specific actin and sarcomeric actin. Electron microscopy revealed paranuclear thick and thin filaments. These findings confirm striated muscle differentiation and a diagnosis of glycogen-rich clear cell rhabdomyosarcoma. The tumour recurred in a cervical lymph node after 2 years. This is yet another tumour that must be considered in the differential diagnosis of clear cell tumours of the head and neck region.

Actin Cytoskeleton↗

PubMedDate not supplied

Perineurioma: an unusual cause of an external auditory canal polyp.

A 33-year-old woman presented with pruritus of the right ear and on examination was found to have 1 x 1 cm polypoid lesion in the external auditory canal. This was removed and histological examination revealed a well-circumscribed spindle cell lesion arranged in fascicles and whorls. Immunohistochemistry showed the tumor to be EMA-positive but S-100-, desmin-, actin-, and cytokeratin-negative. Ultrastructural examination confirmed perineurial cell origin. There are several lesions in which perineurial cells are present, but a true perineurioma is composed exclusively of perineurial cells. It has characteristic morphological, immunohistochemical and ultrastructural features that help separate it from other nerve and neuroectodermal lesions.

Adult↗

PubMedDate not supplied

C-erbB-2 oncoprotein expression in operable non-small cell lung cancer.

Although c-erbB-2 oncoprotein immunohistochemical expression has been thoroughly studied in a variety of human tumors, its prognostic significance remains unclear. Moreover, differences in assessment criteria further complicate the evaluation of c-erbB-2 as a prognostic marker. In the present study we examined the expression of c-erbB-2 protein in 107 patients suffering from operable (T 1,2-N0, 1 staged) non-small cell lung cancer (30 adenocarcinomas and 69 squamous cell carcinomas) treated with surgery alone. A 3-7 year of follow up (median 45 months) was available for all patients. Paraffin embedded sections were stained with the NCL-CB11 monoclonal antibody using the immunoperoxidase technique. Analysis was based on cytoplasmic reactivity as membrane staining was impossible to assess against this background. Strong positive cytoplasmic staining was identified in 20/107 (19%) of cases, weak in 30/107 (20%) and negative in 57/107 (53%). Results were correlated with patient variables (age,sex) and tumor parameters (T,N-stage, grade, histology, Ki67 proliferation index, p53 and EGFR expression). C-erbB-2 expression was not related to any of these factors. Although c-erbB-2 defined a worse prognosis, univariate analysis of survival did not confirm any statistically significant difference between the c-erbB-2 staining groups (p=0.5). T,N-stage were the only statistically significant prognostic variables. Any contribution of c-erbB-2 to the development of tumour aggressive behaviour in non-small cell lung cancer requires assessment in the specific subgroups of patients.

Adenocarcinoma↗

PubMedDate not supplied

Retinoblastoma (pRb) protein immunoexpression in colorectal cancer.

The level of expression of the cell cycle regulatory proteins is an important facet in determining the malignant state. Fifty consecutive colectomy specimens of colorectal carcinoma in patients over 50 years of age, and 25 cases in patients under 30 years, were examined to ascertain retinoblastoma protein expression and to relate this to clinicopathological features. Retinoblastoma protein (pRb) staining was more intense in well differentiated, low stage carcinomas in the over 50 age group (p < 0.001). On the other hand, in the under 30 age group, pRb staining was seen in all tumours grades and stages. No statistical significance was noted with other clinical or pathological features in both age groups.

Adenocarcinoma↗