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Biomedical subjects

R Carlson

Publications and source records attributed to R Carlson.

At least 55 records · Page 3Linked to original sources
PubMed1993

A search for life on Earth from the Galileo spacecraft.

In its December 1990 fly-by of Earth, the Galileo spacecraft found evidence of abundant gaseous oxygen, a widely distributed surface pigment with a sharp absorption edge in the red part of the visible spectrum, and atmospheric methane in extreme thermodynamic disequilibrium; together, these are strongly suggestive of life on Earth. Moreover, the presence of narrow-band, pulsed, amplitude-modulated radio transmission seems uniquely attributable to intelligence. These observations constitute a control experiment for the serach for extraterrestrial life by modern interplanetary spacecraft.

Atmosphere↗
PubMed1993

Hospital associated costs of treating patients with AIDS.

AIM: To determine the hospital associated costs of caring for patients with AIDS. METHOD: We reviewed the notes of all 40 patients with AIDS admitted to the Auckland Hospital infectious disease unit during 1988. We used the resource utilisation system (RUS) to determine costs. RESULTS: Costs were maximal in the month of diagnosis of AIDS (ca $3500/month), falling thereafter to ca $1000/month until they rose again in the last months of life. Total costs were dominated initially by inpatient costs but subsequently by the costs of medicines. CONCLUSION: The hospital costs associated with the care of AIDS patients are not spaced evenly throughout the illness. Extended life will not increase costs proportionately.

Acquired Immunodeficiency Syndrome↗
PubMed1993

Duplicate reporting of AIDS cases between two neighbouring countries.

AIMS: To measure the extent and characteristics of cases of duplicate notification of diagnoses of Acquired Immunodeficiency Syndrome (AIDS) between Australia and New Zealand. METHODS: Cases of AIDS notified to the national AIDS registries in both Australia and New Zealand were identified, using date of birth and a name code. Cases determined to have been notified to both registries were analysed with respect to exposure category, country of birth and country of death. RESULTS: Twenty-six cases of AIDS were determined to be common to both registries, representing 1.1% of cases notified to the Australian registry and 11.1% of cases notified to the New Zealand registry. Exposure to HIV was attributed to male homosexual contact for all cases, and a history of injecting drug use was reported more frequently to the Australian registry for the cases in common. For the majority of cases notified to both registries, country of birth was recorded as New Zealand. Deaths following AIDS were reported as occurring in both countries with equal frequency.

Acquired Immunodeficiency Syndrome↗
PubMed1992

Thermal desorption cold trap-injection in high-resolution gas chromatography: multivariate optimization of experimental conditions.

In studies of low concentrations of volatile compounds in air, the method of adsorption on porous polymers and determination by thermal desorption cold trap-injection high-resolution gas chromatography is finding increasing application. Factors considered important for injection and chromatographic separation of volatile compounds by this method were investigated with the use of multivariate techniques. For the amount injected on to the chromatographic column, the factors of main importance were found to be the temperature of the injection block, the thickness of the internal coating of the cold trap and the flow-rate. Strong interaction effects were noted. For the sharpness of the chromatographic peaks, the flow-rate was the most important factor.

Air Microbiology↗
PubMed1992

The major histocompatibility complex class I antigen-binding protein p88 is the product of the calnexin gene.

A 90-kDa phosphoprotein (p90) of the endoplasmic reticulum was identified by a monoclonal antibody generated against human hepatoma cells. Pulse-chase experiments with [32P]phosphate and [35S]methionine demonstrated that p90 formed both stable and transient complexes with other cellular proteins, suggesting its role as a molecular chaperone. This protein associates with heavy chains of major histocompatibility complex class I proteins, suggesting that it is the human homolog of the recently described 88-kDa protein that transiently associates with murine class I molecules in the endoplasmic reticulum. The p90 protein also associates in B lymphocytes with membrane immunoglobulin mu heavy chains and may serve as a chaperone for many membrane-bound polypeptides. A partial human p90 cDNA was cloned from a lambda gt11 expression library and identified as the human homolog of calnexin, a major canine calcium-binding protein found to be associated with the signal-sequence receptor in endoplasmic reticulum membranes.

Amino Acid Sequence↗
PubMed1992

Monoclonal antibodies specific for mercuric ions.

Monoclonal antibodies (mAbs) that react with soluble mercuric ions have been produced by injection of BALB/c mice with a hapten-carrier complex designed to maximize exposure of the metal to the immune system. Three hybridomas producing antibodies that reacted with bovine serum albumin (BSA)-glutathione-HgCl, but not with BSA-glutathione, were isolated from the spleen of a mouse given multiple injections with glutathione-HgCl conjugated to keyhole limpet hemocyanin. Stable subclones were established from two of these antibodies, designated mAb 4A10 and mAb 1F10. The binding of both antibodies to immobilized BSA-glutathione-HgCl was inhibited by soluble HgCl2, and dissociation constants for mercuric chloride binding were 2.3 and 3.7 nM for mAbs 4A10 and 1F10, respectively. Both antibodies bound mercuric acetate with similar affinities, demonstrating that the antibodies were capable of binding to mercuric ions in the presence of a different counterion than the one used in the immunogen. Reactions were not observed with other metal cations by either antibody. These data demonstrate the successful induction of antibodies that react very specifically with mercuric ions in solution regardless of the presence of a carrier.

Amino Acid Sequence↗
PubMed1992

Multicenter phase II trial of brequinar sodium in patients with advanced squamous-cell carcinoma of the head and neck.

A total of 19 patients with advanced squamous-cell carcinoma of the head and neck who had not previously been exposed to chemotherapy were treated with brequinar sodium as first-line chemotherapy. Brequinar was given intravenously at a median weekly dose of 1,200 mg/m2. The toxicity was moderate, with 7 patients (37%) experiencing grade 3 or 4 toxicity. In all, 16 patients who were evaluable for efficacy showed no objective response. We conclude that brequinar given at this dose and on this schedule has no significant activity in advanced squamous-cell carcinoma of the head and neck.

Adult↗
PubMed1992

Nurse-physician collaboration: solving the nursing shortage crisis.

The current severe nursing shortage in the United States has many causes and its solution requires new strategies. Collaboration among the American Association of Critical-Care Nurses (AACN), the Society of Critical Care Medicine (SCCM) and the American College of Cardiology (ACC) has provided a model for the multidisciplinary approach needed. Nurse-physician collaboration is an important strategy to address the ongoing shortage.

Critical Care↗
PubMed1992

Multicenter phase II trial of brequinar sodium in patients with advanced melanoma.

Seventeen patients with advanced melanoma and no prior exposure to chemotherapy were treated with brequinar sodium as first-line chemotherapy. Brequinar was given at a median weekly dose of 1200 mg/m2 intravenously. In 14 patients evaluable for efficacy, there were no objective responses. The toxicity was moderate. We conclude that, at this dose and schedule, brequinar has no activity in advanced melanoma.

Adult↗
PubMed1992

100 percent smokefree New Jersey restaurants.

The overwhelming majority of New Jerseyans are not smokers and they want and deserve protection. Smokefree policies benefit restaurants. Smokers, too, support controls. Legal action, education, and public demand will make New Jersey restaurants smokefree.

Humans↗
PubMed1992

Nurse/physician collaboration: solving the nursing shortage crisis.

This summary article discusses the status of the nursing shortage in the United States, with emphasis on successful strategies to address it. Liaisons between the American Association of Critical-Care Nurses (AACN) and the Society for Critical Care Medicine, as well as with the American College of Cardiology, are highlighted, with primary emphasis on the strategy of nurse-physician collaboration.

Critical Care↗
PubMed1991

Detection of mercuric ions in water by ELISA with a mercury-specific antibody.

An immunoassay that detects mercuric ions in water at concentrations of 0.5 ppb and above is described. The assay utilizes a monoclonal antibody that binds specifically to mercuric ions immobilized in wells of microtiter plates. Within the range of 0.5-10 ppb mercury, the absorbance in the enzyme-linked immunosorbent assay (ELISA) is linear to the log of the mercuric ion concentration. The quantitation of mercury by ELISA correlates closely with results from cold-vapor atomic absorption. Other divalent metal cations do not interfere with the assay, although there is interference in the presence of 1 mM chloride ions. The optimum pH for mercury detection is 7.0, although 2 ppb mercury can be detected over a wide pH range. The assay is as sensitive as cold-vapor atomic absorption for mercury detection and can be performed with only 100 microliters of sample.

Animals↗
PubMed1990

Trends in hepatitis B notifications 1976-87.

This paper considers trends in hepatitis B notifications in New Zealand during the period 1976 to 1987. It attempts also to demonstrate the fact that although notifications are never comprehensive, even incomplete statistics can give a reasonable and useful indication of trends. Since 1985 most districts have shown a gradual and persistent decline in notification rates. High notification rates are observed from Whangarei, Auckland, South Auckland, Rotorua, Gisborne and Hutt health districts. With a notification rate of 54 per 100,000 population in 1984 Whangarei health district gave the highest rate during this period. These observations are consistent with what is known from other studies about the geographical distribution of hepatitis B markers. Peak notification rates occur in the 15 to 25 age group with highest rates in groups of nonEuropean ethnicity. Notification rates within each district over the eleven year period are compared to the national rates. Further analyses by demographic factors based on information available for the year 1987 are presented. Again the highest rates are observed in groups of nonEuropean ethnicity with the Maori and Pacific Island populations showing rates of 27.2 and 23.0 per 100,000 population respectively. For the eleven year period notification rates for all types of infectious hepatitis are compared to identify secular trends. It is seen that hepatitis A notification rates have declined since the mid-1970s while rates for nonA nonB hepatitis and acute nonspecific hepatitis rose sharply in the early 1980s and have since declined slightly in 1986 and 1987.

Adolescent↗
PubMed1989

Radioimmunolocation of hepatic and pulmonary metastasis of human colon adenocarcinoma.

We have established a large library of monoclonal antibodies against a human hepatoma cell line called FOCUS. One such monoclonal antibody (SF-25) detects a 125-kilodalton cell surface antigen found on FOCUS cells. As both the liver and the colon are of endodermal origin, we examined the possibility of expression in colon adenocarcinomas. This antigen was found in all 23 colon adenocarcinoma tissues surgically obtained but was absent in the adjacent normal mucosal counterpart as determined by a direct radioimmunohistologic technique. In the present study, we have established a model for human metastatic colon adenocarcinoma using the LS 180 cell line. Athymic mice were further immunosuppressed by intravenous injection of anti-NK cell antibodies (antiasialo GM1). After 24 h, mice were injected with LS 180 cells either via the tail vein or into the spleen followed by splenectomy. Macroscopic pulmonary and lymphatic metastasis developed within 2-3 wk after injection of cells and 9 of 10 mice died with advanced metastatic disease 2-3 wk later. In addition, macroscopic hepatic metastases were evident in 4 of 5 mice 3-4 wk after intrasplenic injection. Both hepatic as well as pulmonary and lymphatic tumor spread was localized by nuclear imaging with 125I-SF-25. Furthermore, micrometastases were detected by autoradiography 5-10 days later. Monoclonal antibody SF-25 is a potential candidate for tumor localization and the experimental metastatic colon cancer animal model may be useful for treatment evaluation of monoclonal antibody SF-25 either alone or in combination with other monoclonal antibodies when conjugated to radionucleotides and chemotherapeutic agents.

Adenocarcinoma↗