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Biomedical subjects

R C Loehr

Publications and source records attributed to R C Loehr.

9 recordsLinked to original sources

PAH release during water desorption, supercritical carbon dioxide extraction, and field bioremediation.

Removal rates of polycyclic aromatic hydrocarbons (PAHs) from manufactured gas plant (MGP) soils were determined using water desorption for 120 days and mild supercritical carbon dioxide extraction (SFE) for 200 min. Both techniques were used to compare the changes in desorption rates for individual PAHs from untreated and treated soils that were obtained from a field biotreatment unit after 58, 147, and 343 days. Water desorption profiles (plotted in days) and SFE profiles (plotted in minutes) were very similar regardless of whether a PAH was rapidly or slowly removed. Water and SFE profiles were fit with a simple two-site (fast and slow) model to obtain the fraction of each PAH that was rapidly released (F). There was agreement between the F values obtained from water desorption and SFE for PAHs ranging from naphthalene to benzo[a]pyrene from all soils, with an overall correlation coefficient (r2) of 0.81. F values from water desorption and SFE also agreed with the actual removal of PAHs obtained after 147 and 343 days of field remediation (r2 ca. 0.80). The use of shorter desorption times (2-4 days for water and 20-40 min for SFE) allowed F values to be estimated for all PAHs and showed excellent agreement with the removal of individual PAHs obtained with 147-343 days of field remediation (r2 > 0.9). The comparisons indicate that short-term SFE can provide a reasonable estimate of the fraction of a PAH that is readily released and available for microbial treatment.

Absorption↗

Predicting hydrocarbon removal from thermally enhanced soil vapor extraction systems. 1. Laboratory studies.

Conventional soil vapor extraction (SVE) systems have a limited effectiveness at removing semi-volatile chemicals from soil. Raising chemical vapor pressures by heating soil in-situ can decrease remediation time and help remove semi-volatile chemicals that otherwise would not be removed by conventional SVE. The increased compound removal rate that results from use of thermally enhanced SVE was investigated in laboratory studies. Increased soil temperatures (50-150 degrees C) increased both the rate of removal of the compounds studied and the range of compounds that were removed in column studies. The column studies indicated that if soil temperatures are raised enough to elevate the vapor pressure of a compound above 70 Pa, SVE will remove most of the compound from the soil. Thermally enhanced column study hydrocarbon removal rate constants were shown to have a definable relationship with vapor pressure. The relative removal rate constants also demonstrated an Arrhenius relationship with temperature. Laboratory studies can be used to develop these relationships and the results can be extrapolated within certain temperature ranges and compound types for a given soil.

Environmental Pollution↗

Predicting hydrocarbon removal from thermally enhanced soil vapor extraction systems. 2. Field study.

The effectiveness of a thermally enhanced soil vapor extraction (SVE) system to remove semi-volatile organic chemicals (SVOCs) was investigated in a field study. The data allowed the calculations of SVOC removal rates at several temperatures. A previous laboratory study using the same field soils had developed a relationship between SVOC removal rate constants and inverse temperature. The laboratory and the field SVOC removal rate constants were compared and a linear log-log relationship between the laboratory and the field SVOC removal rate constants resulted. Subsequent analyses indicated that it was possible to use laboratory determined SVOC removal rate relationships to estimate SVOC removal from in situ field soil. The time dependence of SVOC concentration reduction using in situ thermally enhanced SVE systems was also shown.

Environmental Pollution↗

Hazardous solid waste from agriculture.

Large quantities of food processing, crop, forestry, and animal solid wastes are generated in the United States each year. The major components of these wastes are biodegradable. However, they also contain components such as nitrogen, human and animal pathogens, medicinals, feed additives, salts, and certain metals, that under uncontrolled conditions can be detrimental to aquatic, plant, animal, or human life. The most common method of disposal of these wastes is application to the land. Thus the major pathways for transmission of hazards are from and through the soil. Use of these wastes as animal feed also can be a pathway. While at this time there are no crises associated with hazardous materials in agricultural solid wastes, the potential for problems should not be underestimated. Manpower and financial support should be provided to obtain more detailed information in this area, esepcially to better delineate transport and dispersal and to determine and evaluate risks.

Agriculture↗

Filtration method for bacteriophage detection.

A filtration method has been developed which can be used to detect and enumerate phage in low concentrations directly from solution without the need for prior concentration. In this method, a known volume of the phage solution is mixed with a suitable host solution. Samples are filtered through membrane filters; the filter is removed and incubated, and after 24 hr the resultant plaques are counted and the titer is calculated. Escherichia coli B and the coliphage T2 were used in these studies. Host cultures less than 12 hr old produced the best results. Approximately 10(10) host organisms must be present in the sample taken for filtration. To avoid phage reproduction, all steps prior to filtration must be done in less than 45 min. The method was compared with the soft-agar technique and was shown to be less precise but able to measure phage in lower concentrations.

Bacteriophages↗