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Biomedical subjects

R Bruno

Publications and source records attributed to R Bruno.

At least 163 records · Page 9Linked to original sources
PubMed1982

Inhibition of anaerobic glycolysis in bovine retina extracts by salicylate and acetylsalicylate.

1. Na salicylate 31 mM inhibits anaerobic glycolysis from glucose in bovine retina extracts. The formation rate of DAP and GAP increases while that of FDP, G6P, F6P and lactate decreases. All the above modifications are almost completely removed by 1.4 mM NAD+. 2. Bovine retina extracts, preincubated for 1 hr at 0 degrees C with 31 mM Na salicylate show a strongly reduced glycolytic activity. In this system G6P and F6P do accumulate, FDP, DAP, GAP and lactate decrease. These effects are not altered adding 3.5 mM NAD+ to the preincubation mixture. 3. Acetylsalicylate 31 mM inhibits anaerobic glycolysis in crude retina extracts. As the rate of lactate formation decreases, G6P and F6P do accumulate, while FDP, DAP and GAP diminish. 4. Identical modifications are observed adding the inhibitor directly to the incubation mixture, or preincubating it with the extracts at 0 degrees C for 4 hr. 3.5 mM NAD+ does not remove the effects of acetylsalicylate.

Animals↗
PubMed1982

[Method of plasma acetate determination by gas chromatography after ethanol administration in man (author's transl)].

Plasma acetate is extracted using ethanol in alkaline medium, in presence of internal standard. After concentration of the hydroalcoholic phase, the chromatographic analysis is performed utilizing 10% SP 1200 and 1% H3PO4 on chromosorb W. The linearity of the method has been tested from 0.05 to 10 mM/I, its reproducibility is +/- 3.5% for levels ranging from 0.1 to 10 mM/I and +/- 10% for those smaller than 0.1 mM/I. This method is sensitive enough to assess the physiological acetate levels and those obtained after ethanol biotransformation.

Acetates↗
PubMed1982

[Approach to ethanol metabolism in man by studying the concentration-time curves of ethanol, free enzyme and plasma acetate (author's transl)].

The use of a pharmacokinetic model characterized by a new theoretical approach of the enzymatic reaction allows to account for ethanol concentration-time curve (after oral administration in man) and to simulate the evolution of free enzyme concentration. The interpretation of the various phases observed during these kinetics (ethanol, free enzyme) and plasma acetate kinetics (performed in the same study) constitutes an original approach to ethanol metabolism.

Acetates↗
PubMed1982

Blood glucose and tissue glycogen concentrations in normal and deutectomised chickens during the first twelve hours after hatching.

1. Blood glucose, plasma free fatty acids and amino nitrogen, and liver, skeletal muscle, heart, brain and lung glycogen were determined in normal and deutectomised (stripped of yolk sac at hatching) chickens 45 min, 2, 4, 6, 9 or 12 h after hatching. 2. In the normal bird blood glucose decreased by 41% in the first hour after hatching, returning to the hatching value at 12 h. The free fatty acids and the amino nitrogen values did not change much during the 12-h period. Liver glycogen increased by 86% at 2 h, decreasing slightly before returning to hatching value at 12 h. 3. In the deutectomised bird blood glucose was essentially constant and almost equal, during the 12-h period, to that at hatching. Free fatty acids and amino nitrogen were lower after 1 h than at hatching and liver glycogen after 1 h declined to 50% of that at hatching, declining further with time.

Aging↗
PubMed1981

[Proline hydroxylase activity and collagen levels in the lungs of chick embryo during ontogeny].

The tropocollagen in the lungs of chicken embryos increases rapidly between the 13th and 16th day of development, then keeps constant levels and equal ones to those of the adult until the birth. The proline hydroxylase activity is highest in the lung of the embryos of 13 days old, diminishes rapidly between the 13th and 16th day, after that moderately but constantly until the birth, when it is equal to the levels found in the adult. The procollagen has a behaviour comparable to the one described for the proline hydroxylase.

Animals↗
PubMed1981

[NAD glucohydrolase from bovine retina].

The NAD glycohydrolase activity in the retina is very low compared with the one found in the brain. Therefore the retina extracts ahve a sufficiently high NAD level so that they are able to form "in vitro" lactate from glucose in anaerobiosis in presence of only ATP, Mg++ and glucose. The NAD glycohydrolase has been found in the retina in a great extent in the microsomes.

Anaerobiosis↗
PubMed1979

[Lipase and alpha amylase of chick embryo pancreas and of chickens of various ages].

In chicken embryo pancreas on the 19th day the activity of alpha-amylase and especially of lipase is very low; it increases soon after until it reaches its highest level in 36 hours old chick. Then it falls considerably on the 2nd and 10th day of normal diet; finally it starts again: in fact in three months old chickens the activity of alpha-amylase is equal to thirty-six hours old chick's, while lipase's is lower than this last one.

Age Factors↗