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R Brandt

Publications and source records attributed to R Brandt.

173 records · Page 10Linked to original sources
PubMed1971

Ternary Fission.

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Hot Temperature↗
PubMedDate not supplied

Prostaglandin formation from exogenous precursor in homogenates of human cardiac tissue.

The ability of some different components of human cardiac tissue to synthesize prostaglandins (PGs) from exogenous precursor was investigated. Fractions of the cardiac tissue containing either parts of valve cusps and the papillary musculature or some selected tissue components such as myocytes, endocardial elements, endothelial cells and connective tissue were prepared and homogenized. Low-speed supernatants of the various homogenates thus obtained were incubated with [14C]-labelled arachidonate [( 14C]-AA]. [14C]-PGs formed in the incubates were extracted, separated by thin-layer chromatography and quantified using liquid scintillation spectrometry. In the incubates of all the fractions [14C]-AA was converted to [14C]-PGs with a time-dependent yield, most effectively at 3 minutes' incubation time. The "endocardial" and "endothelial" fractions were found to exhibit the highest cyclo-oxygenase activity, the [14C]-AA conversion rate in these incubates being twice as high as in the others. [14C]-labelled PGF2, PGE2 alpha and 6-keto-PGF1 alpha were found to be the principal PG products and there was no evidence of TxB2 formation in any of the incubates. [14C]-6-keto-PGF1 alpha was the main PG formed, constituting about 40% or more of the [14C]-PG activity in the incubates of all the fractions, whereas labelled PGE2 and PGF2 alpha were observed in considerably smaller and nearly equal amounts. The results demonstrate a considerable ability of human cardiac tissue to synthesize prostacyclin (PGI2) and, at the same time, the existence of local differences in tissue cyclo-oxygenase activity, which appears to be significantly higher in the endocardial layer than in the myocardium itself.

6-Ketoprostaglandin F1 alpha↗
PubMedDate not supplied

CDTect-RIA and CDTect-EIA for determination of serum carbohydrate-deficient transferrin compared.

CDTect-RIA and CDTect-EIA for determination of serum carbohydrate-deficient transferrin (CDT) by radioimmunoassay and enzyme immunoassay respectively were tested for equality and precision in four European laboratories. For correlational studies, serum samples with CDT concentrations up to 130 U/l were analysed in accordance with a uniform trial schedule. The regression of CDT values obtained by the two procedures was computed for each laboratory using the method of Passing and Bablok. Slopes and intercepts of the regression functions did not differ significantly from the values 1 or 0, as proved by the corresponding 95% confidence intervals. Precision studies were computed using analysis of variance. For CDT concentrations at the upper reference limit for men, the within-day coefficients of variation (CVs) ranged between 0.7 and 6.4% (median 5.2%) for CDTect-RIA and from 4.3 to 9.2% (median 6.2%) for CDTect-EIA. The corresponding pure between-day CVs were 5.0-18.5% (median 9.8%) and 3.5-14.5% (median 10.9%). The study demonstrates the equality of CDT values obtained by CDTect-RIA and CDTect-EIA. According to this study, the two methods can be used interchangeably without getting fluctuating CDT values, e.g. in longitudinal studies.

Alcoholism↗