[Lymphography: technic, indications and results].
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Biomedical subjects
Publications and source records attributed to R Bourdon.
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Mercury determination in blood and urine can be performed by inductively coupled plasma atomic emission spectroscopy (ICPAES) after dilution in an ammonia buffer and reduction by sodium borohydride. The proposed method does not need an oxidative mineralization. The sample is not nebulized into the torch, but the mercury vapor, after collection in a reactor vial, is swept into the plasma by the argon carrier gas using the described glass apparatus.
Cyanide determination in whole blood can be performed by spectrophotometry after using diffusion coupled with coloration by hydroxocobalamin in a Conway dish. The technique may be accelerated by the use of a heating sheet at 45 degrees C. The method proved to be specific, sensitive, and fast, thus permitting measurements in emergency situations.
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The high number of drug addicts using marijuana (Cannabis sativa var. indica) under various forms has lead research workers to propound many a method for the characterization and assaying of delta9-tetrahydrocannabinol (delta9-THC) and its metabolites in biological fluids. Some of them are based on labelled materials, radioimmuno-chemistry, gas chromatography alone or combined with mass spectrometry. No mention of a purely chemical method has been found in the literature, with the exception of characterizations using thin-layer chromatography and formation of dansylated derivatives (5 to 9, 11 to 14, 16, 17, 18, 22, 23, 26, 31, 32, 34, to 38, 43 to 48, 59).
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Digoxin, digitoxin and acetyldigitoxin are the most widely prescribed of cardiotonic glycosides. Analytical exploration can be performed by a single assay when only one compound is prescribed or by multiple assays in cases of imprecise prescription or when several glycosides are given concomitantly. In addition, the metabolic transformation of digitoxin into digoxin and the presence of endogenous "digitalis-like compounds" mean that a number of precautions must be taken during prescription. These examples underline the ambiguity of the so-called "digitalinaemia" prescriptions and the need for biologists to be supplied with maximum information by clinicians.
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