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Biomedical subjects

R Bolle

Publications and source records attributed to R Bolle.

31 records · Page 2Linked to original sources
PubMed1981

Antigens Common to Scabies and House dust Mites.

Twenty-five antigens were demonstrated in a crude Dermatophagoides farinae (DF) extract by means of crossed immunoelectrophoresis (CIE), using a rabbit anti-DF serum. With the same antiserum, four antigens were demonstrated in a scabies mite preparations, indicating an immunological relationship between these scabies antigens and some of the DF antigens. In tandem CIE experiments the DF antigens were found to be partly identical to three of the scabies antigens. No IgE activity against any of these scabies antigens was evident in pooled sera from patients with house dust mite allergy by crossed radioimmunoelectrophoresis. However, one such patient had IgE antibodies to a DF antigen and a partial identical scabies antigen.

Animals↗
PubMed1980

IgE antibodies to house dust mite in patients with scabies.

Of 135 patients with scabies, forty-seven (35%) had a positive (Classes 1--4) radioallergosorbent test (RAST) to Dermatophagoides pteronyssinus (DP). Elevated serum IgE concentrations were found in sixty-one (45%) of the patients. Atopic disease was confirmed in twenty-eight (23%) of the 120 patients who completed a medical questionnaire satisfactorily. In a control group, well-matched for age and sex, of 201 employees at a local bank, only four (2%) had a positive RAST to DP, and eight (4%) had raised serum IgE levels. Atopic disease was confirmed in eighteen (9%) of them. These observations indicate that scabies infection stimulates the production of IgE antibodies, even in non-atopic individuals, and may suggest that IgE antibodies against the human pathogenic mite Sarcoptes scabiei cross-react with DP. Scabies infection in addition often seems to be associated with atopic disease.

Adolescent↗
PubMed1980

In vitro demonstration of specific immunological hypersensitivity to scabies mite.

An extract prepared from 200 adult female scabies mites was used for prick and intracutaneous testing in twelve patients with previous scabies infestation, as well as in six healthy controls and three persons with skin sensitivity to Dermatophagoides pteronyssinus who had never had scabies before. Seven individuals who had had scabies less than a year prior to the testing had positive intracutaneous (immediate type) reactions, whereas all the five who had had scabies more than a year before had negative reactions. The prick tests were negative in all cases. No skin reactions could be evoked in the controls. The passive transfer, or Prausnitz-Küstner, test was positive with the scabies mite extract and serum from three of five patients tested on one healthy individual, and with both of two sera tested on a second healthy individual. These findings indicate that immediate type hypersensitivity reactions may occur with scabies infection.

Adolescent↗
PubMed1977

A new well-characterized, purified allergen preparation from timothy pollen. II. Allergenic in vivo and vitro properties.

A purified allergen preparation, Timothy N, from timothy pollen and a crude commercial extract, Timothy O, prepared from the same pollen material, were tested in tenfold dilutions on 59 adults with allergic rhinitis and on 29 children with bronchial asthma. All patients were allergic to grass pollen. Using the prick and intracutaneous methods, dose-response tablets were established for the two preparations showing a good correlation. Nasal provocation tests on the adult patients and bronchial provocation tests on the children gave positive reactions in all cases when using Timothy N. No irritant effect resulting in non specific reactions were elicited. The availability of stable, freeze-dried and purified allergen preparations supplied in different vials with defined allergenic activities fulfils the clinical demands for a modern diagnostic allergen preparation.

Adolescent↗
PubMed1977

A new well-characterized, purified allergen preparation from timothy pollen. III. Immunological properties in the human immunoglobulin E system.

The following methods were used in vitro to characterize and quantitate the allergenic activity of a purified allergen preparation, Timothy N, from timothy pollen: radioallergosorbent test (RAST), direct-titration RAST and RAST inhibition. Vials containing freeze-dried and standardized quantities of this preparation showed good uniformity and accuracy with regard to the allergenic activity. The stability of the preparation in solution and in the freeze-dried state was tested by RAST inhibition and was found to be acceptable. Using the same method, the allergenic activity of Timothy N was compared to the allergenic activity of a crude extract, Timothy O, indicating a discrepancy which was not observed in vivo.

Allergens↗