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Biomedical subjects

R Boes

Publications and source records attributed to R Boes.

3 recordsLinked to original sources
PubMed1993

Scrape-loading: a simple method to induce chromosomal aberrations with restriction enzymes in CHO cells.

The restriction endonucleases AluI and EcoRI induce chromosomal aberrations in Chinese hamster ovary (CHO) cells when applied via scrape-loading. The frequencies of chromosomal aberrations induced by AluI are similar to the ones induced when the enzyme is applied by other methods to introduce it into cells (electroporation, glycerol, sorbitol). EcoRI produces lower frequencies of aberrations when applied via scrape-loading, as compared to the application in the presence of high concentrations of glycerol, which may reflect relaxed activities under the latter conditions.

Animals↗
PubMed1992

Uptake of the restriction endonuclease AluI by Chinese hamster ovary cells measured by frequencies of induced chromosomal aberrations: effect of hypertonic concentrations of glycerol and sorbitol.

Chinese hamster ovary cells were exposed to the restriction endonuclease AluI in the presence of 2.2 M glycerol or 0.9 M sorbitol using different treatment regimens. Induced chromosomal aberrations were taken as indication for cellular uptake of AluI. The results indicate that cellular uptake of AluI is mediated by vesicles formed during transition of hypertonic to isotonic conditions. Addition of medium leads to breakdown of these vesicles and to liberation of AluI molecules which eventually may reach the chromatin.

Animals↗