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Biomedical subjects

R Bitsch

Publications and source records attributed to R Bitsch.

32 records · Page 2Linked to original sources
PubMed1982

Thiamin Metabolism in the rat during long-term alcohol administration. 1. Communication: ethanol induced changes at optimal thiamin supply.

The effect of ethanol administration on the phosphorylation of thiamin, and on the transketolase activity (TKA) in erythrocytes, heart and liver, and on the thiamin excretion in urine was investigated in rats optimally supplied with thiamin over a period of 16 weeks. Ethanol ingestion resulted in a diminished concentration of thiamin (T), thiaminmonophosphate (TMP), thiamindiphosphate (TDP) and thiamintriphosphate (TTP) in blood and organs and in a reduced excretion rate of T in urine due to an impaired intestinal absorption. An ethanol induced alteration of the degree of thiamin phosphorylation became evident only after 16 weeks resulting in a decline of T and TMP in blood and of T in heart to undetectable amounts and in an enhancement of the TDP- and TTP-pool. Despite an increasing content of TDP in erythrocytes the TKA was lowered and the alpha-TK was enhanced during the test period suggesting a partially inhibited formation of the active holoenzyme by alcohol in vivo. However, the lowered TKA and the normal alpha-TK in liver are primarily suggestive of an apo-enzyme degeneration influenced by ethanol.

Alcoholism↗
PubMed1982

Thiamin metabolism in the rat during long term alcohol administration.

In order to test the effect of a long term alcohol administration on the thiamin metabolism in blood, heart and liver under suboptimal supply, an experiment with rats was carried out over a period of 16 weeks. The suboptimal thiamin supply became visible mainly in the liver stores which were lowered during the whole test period. The unphosphorylated thiamin (T) of liver and heart was not detectable after 4 weeks up to the end of experiment. On the other hand the total thiamin concentration in the erythrocytes increased from the beginning due to an enhanced thiamin-diphosphate (TDP) and thiamintriphosphate (TTP) pool and T was lowered to undetectable amounts only after 16 weeks. In contrast, the alpha-TK in blood and liver was enhanced only after 2 and 4 weeks and tended to become normal by the end of the test period indicating an apoenzyme degeneration. Alcohol ingestion resulted in a general diminution of the total thiamin and the thiamin phosphates in blood, heart and liver and a reduced thiamin excretion in urine. An alcohol induced shift of the phosphorylation status could be observed only in the liver, but not in the heart and the erythrocytes, leading to a lowered concentration of T and TMP. The results demonstrate that the level of thiamin and thiamin phosphates in blood and organs under suboptimal thiamin supply seems to be more sensitive to chronic alcohol administration than the transketolase activity and the alpha-TK value.

Animals↗
PubMed1979

[Bulk vegetable material in human nutrition (author's transl)].

Intake of bulk vegetable material has diminished in the western countries considerably since the beginning of industrialisation. Lack of bulk substances is being held responsible lately for the increase of diseases of the gastrointestinal tract. It has been shown, that bulk materials increased stool weight and decreased gastrointestinal passage time; it is assumed, that they do have beneficial effects in the treatment of diverticulosis of the colon. It is still controversial, how bulk materials influence mineral metabolism, especially intestinal resorption of iron, calcium and other bivalent cations. Hypocholesterinemic effects of lignin and pectin, which form part of vegetable bulk food, are as yet not well defined. The question is still open, if bulk foods do have prophylactic effects in regard to carcinoma of the colon.

Anticholesteremic Agents↗
PubMed1977

[Thin layer chromatography of riboflavin in urine (author's transl)].

A method is described for determining riboflavin excretion in urine by thin-Layer chromatography. The sample will be preliminarily purified on Permutit T with subsequent separation of riboflavin by thin-layer chromatography on Kieselgel HR with pyridine--acetic acid--water as an eluant. Substances interfering with the fluorescence of riboflavin can be eliminated through the procedure. Riboflavin remission is measured by a chromatogram-spectralphotometer with fluorescence equipment. Between 0.02 to 0.5 mug riboflavin intensity of fluorescence is proportional to the concentration of riboflavin.

Chromatography, Thin Layer↗
PubMed1977

[Biotin supply and pyruvate carboxylase in blood (author's transl)].

Assaying the activation of pyruvate carboxylase in erythrocytes by measuring the enzyme activity before and after addition of biotin and ATP to the reaction mixture enables to estimate the biotin supply in vitro. According to this method in biotin deficient rats the pyruvate carboxylase-activity increased to 70%, while the increase of activity in supplemented animals amounted to 16%.

Animals↗
PubMedDate not supplied

Benfotiamine in treatment of alcoholic polyneuropathy: an 8-week randomized controlled study (BAP I Study).

A three-armed, randomized, multicentre, placebo-controlled double-blind study was used to examine the efficacy of benfotiamine vs a combination containing benfotiamine and vitamins B6 and B12 in out-patients with severe symptoms of alcoholic polyneuropathy (Benfotiamine in treatment of Alcoholic Polyneuropathy, BAP I). The study period was 8 weeks and 84 patients fulfilled all the prerequisite criteria and completed the study as planned. Benfotiamine led to significant improvement of alcoholic polyneuropathy. Vibration perception (measured at the tip of the great toe) significantly improved in the course of the study, as did motor function. and the overall score reflecting the entire range of symptoms of alcoholic polyneuropathy. A tendency toward improvement was evident for pain and co-ordination; no therapy-specific adverse effects were seen.

Adult↗